Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital
Objective: Multidrug-resistant bacteria, including carbapenem-resistant Acinetobacter baumannii (CRAB), are considered a major public health threat, particularly those which are responsible for nosocomial infections. This study aimed to investigate the molecular mechanism of carbapenem resistance an...
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Elsevier
2025-01-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004570 |
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| author | Tania Nawfal Dagher Linda Hadjadj Fadi Bittar Fadi Fenianos Elias Abdo Jean-Marc Rolain Charbel Al-Bayssari |
| author_facet | Tania Nawfal Dagher Linda Hadjadj Fadi Bittar Fadi Fenianos Elias Abdo Jean-Marc Rolain Charbel Al-Bayssari |
| author_sort | Tania Nawfal Dagher |
| collection | DOAJ |
| description | Objective: Multidrug-resistant bacteria, including carbapenem-resistant Acinetobacter baumannii (CRAB), are considered a major public health threat, particularly those which are responsible for nosocomial infections. This study aimed to investigate the molecular mechanism of carbapenem resistance and the clonal relationship of CRAB isolates of a probable outbreak in the intensive care unit (ICU) of Saydet Zgharta University Medical Center, in north Lebanon. Methods: Thirty-two non-duplicate CRAB isolates were collected from patients hospitalised in the ICU. Antibiotic susceptibility testing was carried out using the disk diffusion method and carbapenemase-encoding genes were searched for using standard polymerase chain reaction. Epidemiological relatedness was studied using multilocus sequence typing. Results: Polymerase chain reaction and multilocus sequence typing results suggested the presence of two different periods: period 1 (November 2018 to February 2019), where 15 CRAB isolates were collected harbouring the blaOXA-23 and blaOXA-24 genes mainly and belonging to several clones; and period 2 (March to May 2019), considered an outbreak period where 17 carbapenem-resistant isolates were isolated, harbouring mostly the blaNDM-2 gene, never previously described in Lebanon and belonging to ST103. Infection control measures implemented in Saydet Zgharta University Medical Center successfully eradicated the NDM-2-producing CRAB ST103 clone, thus putting an end to this outbreak in the ICU department. Conclusions: This study showed that infection control measures and adequate identification of NDM-producing A. baumannii remain a powerful tool to limit the spread of such resistant micro-organisms. |
| format | Article |
| id | doaj-art-cec67cf34aa4424e8eca871a42112e92 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-cec67cf34aa4424e8eca871a42112e922025-02-08T05:00:27ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-01-01406671Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospitalTania Nawfal Dagher0Linda Hadjadj1Fadi Bittar2Fadi Fenianos3Elias Abdo4Jean-Marc Rolain5Charbel Al-Bayssari6Aix Marseille Université, IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France; IHU Méditerranée Infection, Marseille, FranceAix Marseille Université, IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France; IHU Méditerranée Infection, Marseille, FranceAix Marseille Université, IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France; IHU Méditerranée Infection, Marseille, FranceSaydet Zgharta University Medical Center, Zgharta, LebanonDepartment of Medical Laboratory Sciences, Faculty of Health Sciences, University of Balamand, Tripoli, LebanonAix Marseille Université, IRD, APHM, MEPHI, Faculté de Médecine et de Pharmacie, Marseille, France; IHU Méditerranée Infection, Marseille, FranceDepartment of Medical Laboratory Sciences, Faculty of Health Sciences, University of Balamand, Tripoli, Lebanon; Corresponding author. Mailing address: Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Balamand, P.O. Box 100 Tripoli, Lebanon.Objective: Multidrug-resistant bacteria, including carbapenem-resistant Acinetobacter baumannii (CRAB), are considered a major public health threat, particularly those which are responsible for nosocomial infections. This study aimed to investigate the molecular mechanism of carbapenem resistance and the clonal relationship of CRAB isolates of a probable outbreak in the intensive care unit (ICU) of Saydet Zgharta University Medical Center, in north Lebanon. Methods: Thirty-two non-duplicate CRAB isolates were collected from patients hospitalised in the ICU. Antibiotic susceptibility testing was carried out using the disk diffusion method and carbapenemase-encoding genes were searched for using standard polymerase chain reaction. Epidemiological relatedness was studied using multilocus sequence typing. Results: Polymerase chain reaction and multilocus sequence typing results suggested the presence of two different periods: period 1 (November 2018 to February 2019), where 15 CRAB isolates were collected harbouring the blaOXA-23 and blaOXA-24 genes mainly and belonging to several clones; and period 2 (March to May 2019), considered an outbreak period where 17 carbapenem-resistant isolates were isolated, harbouring mostly the blaNDM-2 gene, never previously described in Lebanon and belonging to ST103. Infection control measures implemented in Saydet Zgharta University Medical Center successfully eradicated the NDM-2-producing CRAB ST103 clone, thus putting an end to this outbreak in the ICU department. Conclusions: This study showed that infection control measures and adequate identification of NDM-producing A. baumannii remain a powerful tool to limit the spread of such resistant micro-organisms.http://www.sciencedirect.com/science/article/pii/S2213716524004570Carbapenem resistanceAcinetobacter baumanniiNDM-2Lebanon |
| spellingShingle | Tania Nawfal Dagher Linda Hadjadj Fadi Bittar Fadi Fenianos Elias Abdo Jean-Marc Rolain Charbel Al-Bayssari Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital Journal of Global Antimicrobial Resistance Carbapenem resistance Acinetobacter baumannii NDM-2 Lebanon |
| title | Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital |
| title_full | Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital |
| title_fullStr | Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital |
| title_full_unstemmed | Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital |
| title_short | Clonal dissemination of an NDM-2-producing Acinetobacter baumannii ST103 clone resulting in an outbreak in an intensive care unit of a Lebanese tertiary care hospital |
| title_sort | clonal dissemination of an ndm 2 producing acinetobacter baumannii st103 clone resulting in an outbreak in an intensive care unit of a lebanese tertiary care hospital |
| topic | Carbapenem resistance Acinetobacter baumannii NDM-2 Lebanon |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004570 |
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