The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner).
Insecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera is a globally distributed crop pest affecting over 300 crop species. H. armigera has rapidly evolved insecticide resistance, making it one of the mos...
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Public Library of Science (PLoS)
2025-01-01
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| Online Access: | https://doi.org/10.1371/journal.pone.0318154 |
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| author | Douglas Amado Eva L Koch Erick M G Cordeiro Wellingson A Araújo Antonio A F Garcia David G Heckel Gabriela Montejo-Kovacevich Henry L North Alberto S Corrêa Chris D Jiggins Celso Omoto |
| author_facet | Douglas Amado Eva L Koch Erick M G Cordeiro Wellingson A Araújo Antonio A F Garcia David G Heckel Gabriela Montejo-Kovacevich Henry L North Alberto S Corrêa Chris D Jiggins Celso Omoto |
| author_sort | Douglas Amado |
| collection | DOAJ |
| description | Insecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera is a globally distributed crop pest affecting over 300 crop species. H. armigera has rapidly evolved insecticide resistance, making it one of the most damaging pests worldwide. Understanding the genetic basis of insecticide resistance provides insights to develop tools, such as molecular markers, that can be used to slow or prevent the evolution of resistance. We explore the genetic architecture of H. armigera resistance to a widely used insecticide, flubendiamide, using two complementary approaches: genome-wide association studies (GWAS) in wild-caught samples and quantitative trait locus (QTL) mapping in a controlled cross of susceptible and resistant laboratory strains. Both approaches identified one locus on chromosome 2, revealing two SNPs within 976 bp that can be used to monitor field resistance to flubendiamide. This was the only region identified using linkage mapping, though GWAS revealed additional sites associated with resistance. Other loci identified by GWAS in field populations contained known insecticide detoxification genes from the ATP-binding cassette family, ABCA1, ABCA3, ABCF2 and MDR1. Our findings revealed an oligogenic genetic architecture, contrasting previous reports of monogenic resistance associated with the ryanodine receptor. This work elucidates the genetic basis of rapidly evolving insecticide resistance and will contribute to developing effective insecticide resistance management strategies. |
| format | Article |
| id | doaj-art-cfcb8713b1944f3bbdd4a6bbb58aaa95 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-cfcb8713b1944f3bbdd4a6bbb58aaa952025-02-07T05:30:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031815410.1371/journal.pone.0318154The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner).Douglas AmadoEva L KochErick M G CordeiroWellingson A AraújoAntonio A F GarciaDavid G HeckelGabriela Montejo-KovacevichHenry L NorthAlberto S CorrêaChris D JigginsCelso OmotoInsecticide resistance is a major problem in food production, environmental sustainability, and human health. The cotton bollworm Helicoverpa armigera is a globally distributed crop pest affecting over 300 crop species. H. armigera has rapidly evolved insecticide resistance, making it one of the most damaging pests worldwide. Understanding the genetic basis of insecticide resistance provides insights to develop tools, such as molecular markers, that can be used to slow or prevent the evolution of resistance. We explore the genetic architecture of H. armigera resistance to a widely used insecticide, flubendiamide, using two complementary approaches: genome-wide association studies (GWAS) in wild-caught samples and quantitative trait locus (QTL) mapping in a controlled cross of susceptible and resistant laboratory strains. Both approaches identified one locus on chromosome 2, revealing two SNPs within 976 bp that can be used to monitor field resistance to flubendiamide. This was the only region identified using linkage mapping, though GWAS revealed additional sites associated with resistance. Other loci identified by GWAS in field populations contained known insecticide detoxification genes from the ATP-binding cassette family, ABCA1, ABCA3, ABCF2 and MDR1. Our findings revealed an oligogenic genetic architecture, contrasting previous reports of monogenic resistance associated with the ryanodine receptor. This work elucidates the genetic basis of rapidly evolving insecticide resistance and will contribute to developing effective insecticide resistance management strategies.https://doi.org/10.1371/journal.pone.0318154 |
| spellingShingle | Douglas Amado Eva L Koch Erick M G Cordeiro Wellingson A Araújo Antonio A F Garcia David G Heckel Gabriela Montejo-Kovacevich Henry L North Alberto S Corrêa Chris D Jiggins Celso Omoto The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner). PLoS ONE |
| title | The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner). |
| title_full | The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner). |
| title_fullStr | The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner). |
| title_full_unstemmed | The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner). |
| title_short | The genetic architecture of resistance to flubendiamide insecticide in Helicoverpa armigera (Hübner). |
| title_sort | genetic architecture of resistance to flubendiamide insecticide in helicoverpa armigera hubner |
| url | https://doi.org/10.1371/journal.pone.0318154 |
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