Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study
Abstract There has been increasing interest in utilizing biofluids in combination with high-throughput omics to identify biomarkers, to unravel mechanisms underlying internal physiological environments. Interstitial fluid (ISF) is noted as a high potential biofluid for continuous monitoring of cellu...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2025-02-01
|
| Series: | Discover Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s42452-025-06488-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861761239941120 |
|---|---|
| author | Yu Sakamoto Naoto Fujii Junko Shibato Ravi Gupta Cheol Woo Min Sun Tae Kim Ranjith Kumar Bakku Randeep Rakwal |
| author_facet | Yu Sakamoto Naoto Fujii Junko Shibato Ravi Gupta Cheol Woo Min Sun Tae Kim Ranjith Kumar Bakku Randeep Rakwal |
| author_sort | Yu Sakamoto |
| collection | DOAJ |
| description | Abstract There has been increasing interest in utilizing biofluids in combination with high-throughput omics to identify biomarkers, to unravel mechanisms underlying internal physiological environments. Interstitial fluid (ISF) is noted as a high potential biofluid for continuous monitoring of cellular microenvironment where various metabolic processes occur. This pilot study collected intradermal dialysate, reflective of ISF conditions, from human dorsal forearm (n = 1) using a novel vented-intradermal microdialysis probe, to analyze proteins, isolate them, and characterize their functions. Dialysate samples were checked for protein extracted in LB-TT, SDS-PAGE, and by LC–MS/MS. A protein–protein interaction (PPI) network was constructed with genes from STRING using Cytoscape. We identified a total of 1149 proteins. Network analysis revealed 228 proteins that were successfully identified with the PPI information in the database, where 10 were identified as highly connected proteins with 80 first neighbors. Conclusions must be drawn carefully given that skin dialysate was only collected from one participant in this pilot study. Nonetheless, skin dialysate may serve as an alternative for blood, yet further research is vital to clarify its potential for real-time continuous monitoring applications within sports science research and practices. Article Highlights Intradermal microdialysis approach to collect human dorsal forearm dialysate allows for the collection of high-quality proteins. Proteins, previously identified in other biofluids (blood and saliva), were also identified in human dorsal forearm skin dialysate samples (n = 1). Speculations and conclusions must be drawn with careful consideration regarding the limitations of this biofluid. |
| format | Article |
| id | doaj-art-d06ec7bc0b5c43d4bc096b2f6496c80f |
| institution | Kabale University |
| issn | 3004-9261 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Applied Sciences |
| spelling | doaj-art-d06ec7bc0b5c43d4bc096b2f6496c80f2025-02-09T12:49:50ZengSpringerDiscover Applied Sciences3004-92612025-02-017211510.1007/s42452-025-06488-2Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot studyYu Sakamoto0Naoto Fujii1Junko Shibato2Ravi Gupta3Cheol Woo Min4Sun Tae Kim5Ranjith Kumar Bakku6Randeep Rakwal7Graduate School of Comprehensive Human Sciences, University of TsukubaAdvanced Research Initiative for Human High Performance (ARIHHP), University of TsukubaDepartment of Functional Morphology, Shonan University of Medical SciencesCollege of General Education, Kookmin UniversityDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National UniversityResource Circulation Technology Research Institute, Euglena Co. Ltd.Institute of Health and Sport Sciences, University of TsukubaAbstract There has been increasing interest in utilizing biofluids in combination with high-throughput omics to identify biomarkers, to unravel mechanisms underlying internal physiological environments. Interstitial fluid (ISF) is noted as a high potential biofluid for continuous monitoring of cellular microenvironment where various metabolic processes occur. This pilot study collected intradermal dialysate, reflective of ISF conditions, from human dorsal forearm (n = 1) using a novel vented-intradermal microdialysis probe, to analyze proteins, isolate them, and characterize their functions. Dialysate samples were checked for protein extracted in LB-TT, SDS-PAGE, and by LC–MS/MS. A protein–protein interaction (PPI) network was constructed with genes from STRING using Cytoscape. We identified a total of 1149 proteins. Network analysis revealed 228 proteins that were successfully identified with the PPI information in the database, where 10 were identified as highly connected proteins with 80 first neighbors. Conclusions must be drawn carefully given that skin dialysate was only collected from one participant in this pilot study. Nonetheless, skin dialysate may serve as an alternative for blood, yet further research is vital to clarify its potential for real-time continuous monitoring applications within sports science research and practices. Article Highlights Intradermal microdialysis approach to collect human dorsal forearm dialysate allows for the collection of high-quality proteins. Proteins, previously identified in other biofluids (blood and saliva), were also identified in human dorsal forearm skin dialysate samples (n = 1). Speculations and conclusions must be drawn with careful consideration regarding the limitations of this biofluid.https://doi.org/10.1007/s42452-025-06488-2Interstitial fluidProtein databaseThermoregulationTemperatureExercise |
| spellingShingle | Yu Sakamoto Naoto Fujii Junko Shibato Ravi Gupta Cheol Woo Min Sun Tae Kim Ranjith Kumar Bakku Randeep Rakwal Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study Discover Applied Sciences Interstitial fluid Protein database Thermoregulation Temperature Exercise |
| title | Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study |
| title_full | Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study |
| title_fullStr | Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study |
| title_full_unstemmed | Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study |
| title_short | Exploration of interstitial fluid proteome in Homo sapiens forearm through a cutting edge intradermal microdialysis and high-throughput proteomics approach: a pilot study |
| title_sort | exploration of interstitial fluid proteome in homo sapiens forearm through a cutting edge intradermal microdialysis and high throughput proteomics approach a pilot study |
| topic | Interstitial fluid Protein database Thermoregulation Temperature Exercise |
| url | https://doi.org/10.1007/s42452-025-06488-2 |
| work_keys_str_mv | AT yusakamoto explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT naotofujii explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT junkoshibato explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT ravigupta explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT cheolwoomin explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT suntaekim explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT ranjithkumarbakku explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy AT randeeprakwal explorationofinterstitialfluidproteomeinhomosapiensforearmthroughacuttingedgeintradermalmicrodialysisandhighthroughputproteomicsapproachapilotstudy |