Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction
Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans. Objective: This research aimed to determine the sensitivity and specificity of real-time pol...
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Indonesian Society for Biochemistry and Molecular Biology
2024-08-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/159 |
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| author | Muktiningsih Nurjayadi Gladys Indira Putri Jefferson Lynford Declan Ismaya Krisdawati Dandy Akbar Juliansyah Maharanianska Azzahra Irvan Maulana Irma Ratna Kartika Fera Kurniadewi Tiara Fahriza Adinda Myra Amalia Putri Ayu Berkahingrum Atikah Nur Rahmawati Rosita Gio Anggraeni Dalia Sukmawati Sri Rahayu Vira Saamia I Made Wiranatha Bassam Abomoelak Hesham Ali El-Enshasy |
| author_facet | Muktiningsih Nurjayadi Gladys Indira Putri Jefferson Lynford Declan Ismaya Krisdawati Dandy Akbar Juliansyah Maharanianska Azzahra Irvan Maulana Irma Ratna Kartika Fera Kurniadewi Tiara Fahriza Adinda Myra Amalia Putri Ayu Berkahingrum Atikah Nur Rahmawati Rosita Gio Anggraeni Dalia Sukmawati Sri Rahayu Vira Saamia I Made Wiranatha Bassam Abomoelak Hesham Ali El-Enshasy |
| author_sort | Muktiningsih Nurjayadi |
| collection | DOAJ |
| description |
Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans.
Objective: This research aimed to determine the sensitivity and specificity of real-time polymerase chain reaction (rt-PCR) using the flgL primer pair to detect V. alginolyticus bacteria in seafood.
Methods: The rt-PCR method was used to detect V. alginolyticus quickly, specifically, and sensitively. The flgL primer pair was evaluated for amplicon length, Ct value, Tm value, and its ability to differentiate between target and non-target bacteria. In this research, the samples tested were red snapper and blood clams.
Results: The flgL primer produced an amplicon length of 224 bp. At 50 ng concentration, it yielded a Ct value of approximately 11.00 and a Tm of approximately 83°C. The flgL primer successfully differentiated between target and non-target bacteria. In sensitivity tests, it detected V. alginolyticus at concentrations as low as 1.86 x 10-3 ng/µL. Detection in seafood samples was also successful.
Conclusion: The rt-PCR assay using the flgL primer pair effectively detects Vibrio alginolyticus in seafood with high specificity, sensitivity, and rapidity. These findings support its use for rapid and accurate detection of pathogenic bacteria in seafood.
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| format | Article |
| id | doaj-art-d0b16dbb90d14f5cbafdec44131e3531 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-d0b16dbb90d14f5cbafdec44131e35312025-02-08T03:04:48ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222024-08-017110.32889/actabioina.159Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reactionMuktiningsih Nurjayadi0Gladys Indira Putri1Jefferson Lynford Declan2Ismaya Krisdawati3Dandy Akbar Juliansyah4Maharanianska Azzahra5Irvan Maulana6Irma Ratna Kartika7Fera Kurniadewi8Tiara Fahriza9Adinda Myra Amalia Putri10Ayu Berkahingrum11Atikah Nur Rahmawati12Rosita Gio Anggraeni13Dalia Sukmawati14Sri Rahayu15Vira Saamia16I Made Wiranatha17Bassam Abomoelak18Hesham Ali El-Enshasy19Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, East Jakarta 13220, IndonesiaCenter Forensic Laboratory of the Criminal Investigation, Police of the Republic of Indonesia, Cipambuan Babakan Madang, Bogor 1681, IndonesiaCenter Forensic Laboratory of the Criminal Investigation, Police of the Republic of Indonesia, Cipambuan Babakan Madang, Bogor 1681, IndonesiaArnold Palmer Hospital Pediatric Specialty Diagnostic Laboratory, Orlando, FL 32806, USAInnovation Center in Agritechnology for Advanced Bioprocessing (ICA), Universiti Teknologi Malaysia (UTM), Pagoh, Johor, Malaysia Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans. Objective: This research aimed to determine the sensitivity and specificity of real-time polymerase chain reaction (rt-PCR) using the flgL primer pair to detect V. alginolyticus bacteria in seafood. Methods: The rt-PCR method was used to detect V. alginolyticus quickly, specifically, and sensitively. The flgL primer pair was evaluated for amplicon length, Ct value, Tm value, and its ability to differentiate between target and non-target bacteria. In this research, the samples tested were red snapper and blood clams. Results: The flgL primer produced an amplicon length of 224 bp. At 50 ng concentration, it yielded a Ct value of approximately 11.00 and a Tm of approximately 83°C. The flgL primer successfully differentiated between target and non-target bacteria. In sensitivity tests, it detected V. alginolyticus at concentrations as low as 1.86 x 10-3 ng/µL. Detection in seafood samples was also successful. Conclusion: The rt-PCR assay using the flgL primer pair effectively detects Vibrio alginolyticus in seafood with high specificity, sensitivity, and rapidity. These findings support its use for rapid and accurate detection of pathogenic bacteria in seafood. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/159Vibrio alginolyticusseafoodflgL primerreal-time polymerase chain reactionsensitivityspecifity |
| spellingShingle | Muktiningsih Nurjayadi Gladys Indira Putri Jefferson Lynford Declan Ismaya Krisdawati Dandy Akbar Juliansyah Maharanianska Azzahra Irvan Maulana Irma Ratna Kartika Fera Kurniadewi Tiara Fahriza Adinda Myra Amalia Putri Ayu Berkahingrum Atikah Nur Rahmawati Rosita Gio Anggraeni Dalia Sukmawati Sri Rahayu Vira Saamia I Made Wiranatha Bassam Abomoelak Hesham Ali El-Enshasy Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction Acta Biochimica Indonesiana Vibrio alginolyticus seafood flgL primer real-time polymerase chain reaction sensitivity specifity |
| title | Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction |
| title_full | Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction |
| title_fullStr | Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction |
| title_full_unstemmed | Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction |
| title_short | Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction |
| title_sort | rapid detection of vibrio alginolyticus in seafood using the flgl gene and real time polymerase chain reaction |
| topic | Vibrio alginolyticus seafood flgL primer real-time polymerase chain reaction sensitivity specifity |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/159 |
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