Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant
Putat leaves (Planchonia valida) are a plant that is traditionally used by people in the Tanjung Lanjut areas, Jambi as fresh vegetables, and as a traditional medicine to treat skin diseases and restore health. This identifies that the putat plant contains chemical compounds that have the potential...
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Universitas Jambi
2022-12-01
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| Online Access: | https://online-journal.unja.ac.id/chp/article/view/41634 |
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| author | Faizar Farid Vera Lorensya Br Sembiring Nelson Nelson Muhammad Furqon Novryan Saputra Silvi Ayudiah Putri Indra Lasmana Tarigan Madyawati Latief |
| author_facet | Faizar Farid Vera Lorensya Br Sembiring Nelson Nelson Muhammad Furqon Novryan Saputra Silvi Ayudiah Putri Indra Lasmana Tarigan Madyawati Latief |
| author_sort | Faizar Farid |
| collection | DOAJ |
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Putat leaves (Planchonia valida) are a plant that is traditionally used by people in the Tanjung Lanjut areas, Jambi as fresh vegetables, and as a traditional medicine to treat skin diseases and restore health. This identifies that the putat plant contains chemical compounds that have the potential to have antioxidant activity. Extraction was carried out using n-hexane and ethyl acetate solvents using multistage maceration. Next, phytochemical screening and antioxidant activity tests are carried out to obtain information about fractions that can potentially proceed to the isolation stage. In the maceration process, an extract percentage was obtained with a yield value of 1.39%. Next, isolation was carried out using vacuum liquid chromatography. The isolates obtained were then characterized using UV-Vis and FT-IR spectrophotometer instruments. The isolate obtained was in the form of a wet solid, and if seen through characterization, it belonged to the class of steroid compounds; it was suspected to be an ergosterol compound. Antioxidants are known to have the ability to inhibit and reduce the presence of free radicals in the human body. In this study, antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picrihidazil) method on F1 isolates. The F1 isolate obtained was proven to have antioxidant activity with a value of 99.2265 ppm, which is classified in the strong antioxidant activity range.
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| format | Article |
| id | doaj-art-d16d2bb7c12845e8a059b192d7ab71f5 |
| institution | Kabale University |
| issn | 2503-4588 |
| language | Indonesian |
| publishDate | 2022-12-01 |
| publisher | Universitas Jambi |
| record_format | Article |
| series | Chempublish Journal |
| spelling | doaj-art-d16d2bb7c12845e8a059b192d7ab71f52025-02-12T08:37:03ZindUniversitas JambiChempublish Journal2503-45882022-12-016410.22437/chp.v6i4.41634Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as AntioxidantFaizar Farid0Vera Lorensya Br Sembiring1Nelson Nelson2Muhammad Furqon Novryan Saputra3Silvi Ayudiah Putri4Indra Lasmana Tarigan5Madyawati Latief6Department of Chemistry, Faculty of Science and Technology, Universitas Jambi, INDONESIADepartment of Chemistry, Faculty of Science and Technology, Universitas Jambi, INDONESIADepartment of Chemistry, Faculty of Science and Technology, Universitas Jambi, INDONESIADepartment of Chemistry, Faculty of Science and Technology, Universitas Jambi, IndonesiaDepartment of Chemistry, Faculty of Science and Technology, Universitas Jambi, INDONESIADepartment of Chemistry, Faculty of Science and Technology, Universitas Jambi, INDONESIADepartment of Chemistry, Faculty of Science and Technology, Universitas Jambi, INDONESIA Putat leaves (Planchonia valida) are a plant that is traditionally used by people in the Tanjung Lanjut areas, Jambi as fresh vegetables, and as a traditional medicine to treat skin diseases and restore health. This identifies that the putat plant contains chemical compounds that have the potential to have antioxidant activity. Extraction was carried out using n-hexane and ethyl acetate solvents using multistage maceration. Next, phytochemical screening and antioxidant activity tests are carried out to obtain information about fractions that can potentially proceed to the isolation stage. In the maceration process, an extract percentage was obtained with a yield value of 1.39%. Next, isolation was carried out using vacuum liquid chromatography. The isolates obtained were then characterized using UV-Vis and FT-IR spectrophotometer instruments. The isolate obtained was in the form of a wet solid, and if seen through characterization, it belonged to the class of steroid compounds; it was suspected to be an ergosterol compound. Antioxidants are known to have the ability to inhibit and reduce the presence of free radicals in the human body. In this study, antioxidant activity was tested using the DPPH (2,2-diphenyl-1-picrihidazil) method on F1 isolates. The F1 isolate obtained was proven to have antioxidant activity with a value of 99.2265 ppm, which is classified in the strong antioxidant activity range. https://online-journal.unja.ac.id/chp/article/view/41634Antioxidant ActivityIsolationPutat leaves |
| spellingShingle | Faizar Farid Vera Lorensya Br Sembiring Nelson Nelson Muhammad Furqon Novryan Saputra Silvi Ayudiah Putri Indra Lasmana Tarigan Madyawati Latief Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant Chempublish Journal Antioxidant Activity Isolation Putat leaves |
| title | Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant |
| title_full | Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant |
| title_fullStr | Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant |
| title_full_unstemmed | Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant |
| title_short | Isolation of Bioactive Compound in Methanolic Extract of Putat Leaves (Planchonia valida) as Antioxidant |
| title_sort | isolation of bioactive compound in methanolic extract of putat leaves planchonia valida as antioxidant |
| topic | Antioxidant Activity Isolation Putat leaves |
| url | https://online-journal.unja.ac.id/chp/article/view/41634 |
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