The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36)
Objective: Electroacupuncture at Zusanli (ST36) exerted an effect on brain function by regulating spontaneous brain activity. This study aims to investigate the influence of different post-processing methods of amplitude of low-frequency fluctuation (ALFF) and different frequency band to measure bra...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | NeuroImage: Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666956025000066 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856698149830656 |
|---|---|
| author | YingJie Kang YiLei Chen ZhiGang Gong YanWen Huang Hui Wang Jiling Huang SongHua Zhan YiJing Li WenLi Tan |
| author_facet | YingJie Kang YiLei Chen ZhiGang Gong YanWen Huang Hui Wang Jiling Huang SongHua Zhan YiJing Li WenLi Tan |
| author_sort | YingJie Kang |
| collection | DOAJ |
| description | Objective: Electroacupuncture at Zusanli (ST36) exerted an effect on brain function by regulating spontaneous brain activity. This study aims to investigate the influence of different post-processing methods of amplitude of low-frequency fluctuation (ALFF) and different frequency band to measure brain low-frequency oscillations (LFOs) after electroacupuncture at ST36. Methods: Twenty-eight healthy subjects were recruited and received true electroacupuncture (EA) and sham electroacupuncture (SA) at ST36 respectively. The rs-fMRI scans were performed before and immediately after electroacupuncture. Three post-processing methods were used to evaluate the amplitude of LFOs: fractional ALFF (fALFF), percent amplitude of fluctuation (PerAF) and wavelet-transform ALFF (Wavelet-ALFF). Besides the conventional low-frequency band (0.01–0.1Hz), three additional sub-bands were observed, including slow-6 (0–0.01Hz), slow-5 (0.01–0.027Hz) and slow-4 (0.027–0.073Hz). Paired t-test was conducted to investigate the intra-group differences of three post-processing methods before and after each intervention. In the comparison of inter-group difference, the paired t-test was conducted between SA and EA. Results: Electroacupuncture at ST36 increased LFO amplitudes, with more pronounced effects compared to SA. As compared to SA, the fALFF values in the left superior frontal gyrus and orbital middle frontal gyrus increased after EA. Only the EA group showed an increase in Wavelet-ALFF. PerAF was the most sensitive method for detecting LFO amplitude changes and revealed changes in white matter. Additionally, brain LFO changes were frequency-dependent, with more favorable results observed in the conventional low-frequency band and the slow-5 band. Conclusion: Post-processing methods affect the interpretation of brain effects caused by electroacupuncture at ST36. FALFF was suitable for observing brain LFO differences between EA and SA. Wavelet-ALFF was stable but less sensitive. PerAF was a promising method for investigating LFOs in white matter. The brain LFO changes caused by electroacupuncture at ST36 were frequency-dependent. |
| format | Article |
| id | doaj-art-d17719035ec140e7a79a94c21e04aa0c |
| institution | Kabale University |
| issn | 2666-9560 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | NeuroImage: Reports |
| spelling | doaj-art-d17719035ec140e7a79a94c21e04aa0c2025-02-12T05:32:59ZengElsevierNeuroImage: Reports2666-95602025-03-0151100238The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36)YingJie Kang0YiLei Chen1ZhiGang Gong2YanWen Huang3Hui Wang4Jiling Huang5SongHua Zhan6YiJing Li7WenLi Tan8Department of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Acupuncture, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR ChinaDepartment of Radiology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, PR China; Corresponding author. 528 Zhangheng Road, Shanghai, 201203, PR China.Objective: Electroacupuncture at Zusanli (ST36) exerted an effect on brain function by regulating spontaneous brain activity. This study aims to investigate the influence of different post-processing methods of amplitude of low-frequency fluctuation (ALFF) and different frequency band to measure brain low-frequency oscillations (LFOs) after electroacupuncture at ST36. Methods: Twenty-eight healthy subjects were recruited and received true electroacupuncture (EA) and sham electroacupuncture (SA) at ST36 respectively. The rs-fMRI scans were performed before and immediately after electroacupuncture. Three post-processing methods were used to evaluate the amplitude of LFOs: fractional ALFF (fALFF), percent amplitude of fluctuation (PerAF) and wavelet-transform ALFF (Wavelet-ALFF). Besides the conventional low-frequency band (0.01–0.1Hz), three additional sub-bands were observed, including slow-6 (0–0.01Hz), slow-5 (0.01–0.027Hz) and slow-4 (0.027–0.073Hz). Paired t-test was conducted to investigate the intra-group differences of three post-processing methods before and after each intervention. In the comparison of inter-group difference, the paired t-test was conducted between SA and EA. Results: Electroacupuncture at ST36 increased LFO amplitudes, with more pronounced effects compared to SA. As compared to SA, the fALFF values in the left superior frontal gyrus and orbital middle frontal gyrus increased after EA. Only the EA group showed an increase in Wavelet-ALFF. PerAF was the most sensitive method for detecting LFO amplitude changes and revealed changes in white matter. Additionally, brain LFO changes were frequency-dependent, with more favorable results observed in the conventional low-frequency band and the slow-5 band. Conclusion: Post-processing methods affect the interpretation of brain effects caused by electroacupuncture at ST36. FALFF was suitable for observing brain LFO differences between EA and SA. Wavelet-ALFF was stable but less sensitive. PerAF was a promising method for investigating LFOs in white matter. The brain LFO changes caused by electroacupuncture at ST36 were frequency-dependent.http://www.sciencedirect.com/science/article/pii/S2666956025000066Functional magnetic resonance imagingZusanliElectroacupunctureLow-frequency oscillations amplitudeFrequency band |
| spellingShingle | YingJie Kang YiLei Chen ZhiGang Gong YanWen Huang Hui Wang Jiling Huang SongHua Zhan YiJing Li WenLi Tan The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36) NeuroImage: Reports Functional magnetic resonance imaging Zusanli Electroacupuncture Low-frequency oscillations amplitude Frequency band |
| title | The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36) |
| title_full | The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36) |
| title_fullStr | The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36) |
| title_full_unstemmed | The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36) |
| title_short | The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36) |
| title_sort | influence of post processing methods and frequency bands on rs fmri an example of electroacupuncture at zusanli st36 |
| topic | Functional magnetic resonance imaging Zusanli Electroacupuncture Low-frequency oscillations amplitude Frequency band |
| url | http://www.sciencedirect.com/science/article/pii/S2666956025000066 |
| work_keys_str_mv | AT yingjiekang theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yileichen theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT zhiganggong theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yanwenhuang theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT huiwang theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT jilinghuang theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT songhuazhan theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yijingli theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT wenlitan theinfluenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yingjiekang influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yileichen influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT zhiganggong influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yanwenhuang influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT huiwang influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT jilinghuang influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT songhuazhan influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT yijingli influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 AT wenlitan influenceofpostprocessingmethodsandfrequencybandsonrsfmrianexampleofelectroacupunctureatzusanlist36 |