Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis
Background: The incidence of postoperative cognitive dysfunction (POCD) is higher happening in the elderly. It has been reported electroacupuncture (EA) was beneficial to the treatment of POCD, but its specific regulatory mechanism is still unclear. Methods: Through partial hepatectomy in mice, POCD...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923025000462 |
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| author | Xiaqing Zhang Afen Zhang Rui Li Mingna Jiao Rou Wen Rongzhi Zheng |
| author_facet | Xiaqing Zhang Afen Zhang Rui Li Mingna Jiao Rou Wen Rongzhi Zheng |
| author_sort | Xiaqing Zhang |
| collection | DOAJ |
| description | Background: The incidence of postoperative cognitive dysfunction (POCD) is higher happening in the elderly. It has been reported electroacupuncture (EA) was beneficial to the treatment of POCD, but its specific regulatory mechanism is still unclear. Methods: Through partial hepatectomy in mice, POCD model of mice was established. Baihui acupoint (GV20) was selected for targeted point of EA therapy. Morris water maze (MWM) was applied to evaluate cognitive impairment of mice. HE staining was used to examine cell arrangement and cell morphology in hippocampus of mice. RT-qPCR, western blot and IHC were employed to detect abundance of genes and proteins. MDA, GSH and iron levels was measured by some commercial kits. Results: Our findings revealed that partial hepatectomy surgery impaired learning and memory ability of mice, promoted ferroptosis. inhibited GRX1 and inactivated GSK-3β/Nrf2 pathway. However, EA therapy abolished these effects. In addition, GRX1 silencing and erastin abolished EA-mediated alterations of improving POCD in mice. Conclusion: EA suppressed ferroptosis by regulating GRX1/GSK-3β/Nrf2 pathway to improve postoperative cognitive dysfunction of POCD mice. |
| format | Article |
| id | doaj-art-d360087293bd438fb57913a1870323b1 |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-d360087293bd438fb57913a1870323b12025-02-10T04:33:31ZengElsevierBrain Research Bulletin1873-27472025-03-01222111234Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axisXiaqing Zhang0Afen Zhang1Rui Li2Mingna Jiao3Rou Wen4Rongzhi Zheng5First Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, No.2 Weiyang West Road, Xianyang City, Shaanxi Province 712000, PR ChinaFirst Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, No.2 Weiyang West Road, Xianyang City, Shaanxi Province 712000, PR ChinaFirst Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, No.2 Weiyang West Road, Xianyang City, Shaanxi Province 712000, PR ChinaFirst Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, No.2 Weiyang West Road, Xianyang City, Shaanxi Province 712000, PR ChinaFirst Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, No.2 Weiyang West Road, Xianyang City, Shaanxi Province 712000, PR ChinaCorresponding author.; First Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, No.2 Weiyang West Road, Xianyang City, Shaanxi Province 712000, PR ChinaBackground: The incidence of postoperative cognitive dysfunction (POCD) is higher happening in the elderly. It has been reported electroacupuncture (EA) was beneficial to the treatment of POCD, but its specific regulatory mechanism is still unclear. Methods: Through partial hepatectomy in mice, POCD model of mice was established. Baihui acupoint (GV20) was selected for targeted point of EA therapy. Morris water maze (MWM) was applied to evaluate cognitive impairment of mice. HE staining was used to examine cell arrangement and cell morphology in hippocampus of mice. RT-qPCR, western blot and IHC were employed to detect abundance of genes and proteins. MDA, GSH and iron levels was measured by some commercial kits. Results: Our findings revealed that partial hepatectomy surgery impaired learning and memory ability of mice, promoted ferroptosis. inhibited GRX1 and inactivated GSK-3β/Nrf2 pathway. However, EA therapy abolished these effects. In addition, GRX1 silencing and erastin abolished EA-mediated alterations of improving POCD in mice. Conclusion: EA suppressed ferroptosis by regulating GRX1/GSK-3β/Nrf2 pathway to improve postoperative cognitive dysfunction of POCD mice.http://www.sciencedirect.com/science/article/pii/S0361923025000462ElectroacupunctureGRX1GSK-3βFerroptosisPostoperative cognitive dysfunction |
| spellingShingle | Xiaqing Zhang Afen Zhang Rui Li Mingna Jiao Rou Wen Rongzhi Zheng Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis Brain Research Bulletin Electroacupuncture GRX1 GSK-3β Ferroptosis Postoperative cognitive dysfunction |
| title | Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis |
| title_full | Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis |
| title_fullStr | Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis |
| title_full_unstemmed | Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis |
| title_short | Electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating GRX1/GSK-3β/Nrf2 axis |
| title_sort | electroacupuncture regulates ferroptosis to improve postoperative cognitive dysfunction in mice through mediating grx1 gsk 3β nrf2 axis |
| topic | Electroacupuncture GRX1 GSK-3β Ferroptosis Postoperative cognitive dysfunction |
| url | http://www.sciencedirect.com/science/article/pii/S0361923025000462 |
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