Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy
Abstract Cancer immunotherapy has attracted great attention as a potential therapeutic approach for advanced malignancies due to its promising survival benefits. Comprehension of intricate interactions between the tumor microenvironment (TME) and immune checkpoint inhibitors (ICIs) is crucial for op...
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| Format: | Article |
| Language: | English |
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Springer
2025-02-01
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| Series: | Cancer Immunology, Immunotherapy |
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| Online Access: | https://doi.org/10.1007/s00262-025-03944-1 |
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| _version_ | 1823862244298981376 |
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| author | Minfeng Yang Shiqiang Hou Yao Chen Hongzhao Chen Minjie Chu Song-Bai Liu |
| author_facet | Minfeng Yang Shiqiang Hou Yao Chen Hongzhao Chen Minjie Chu Song-Bai Liu |
| author_sort | Minfeng Yang |
| collection | DOAJ |
| description | Abstract Cancer immunotherapy has attracted great attention as a potential therapeutic approach for advanced malignancies due to its promising survival benefits. Comprehension of intricate interactions between the tumor microenvironment (TME) and immune checkpoint inhibitors (ICIs) is crucial for optimizing and improving immunotherapies. Currently, several experimental strategies are available to monitor this complexity but most of them fail to facilitate real-time monitoring of the immune response such as cellular phagocytosis and cytolysis. Consequently, the application of intravital imaging has been extensively studied in the domain of cancer immunotherapy. Intravital imaging has been proven to be a powerful real-time imaging modality that provides insights into intratumoral immune responses, cellular metabolic signatures, tumor vasculature, and cellular functions. This review aims to provide a comprehensive overview of the latest research on intravital imaging in cancer immunotherapy, especially addressing how intravital imaging sheds light on essential features of tumor immunity, immune infiltrations, tumor angiogenesis, and aids in the clarification of underlying immunotherapeutic mechanisms. Moreover, a variety of labeling tools, imaging windows and models for real-time visualizations of TME are also summarized. We will also investigate the full potential of using intravital imaging to circumvent the limitations of currently available imaging modalities, which hold promise to advent efficient immunotherapy for cancer patients. Graphical abstract |
| format | Article |
| id | doaj-art-d4d52382ef25483f8adc464182717bf5 |
| institution | Kabale University |
| issn | 1432-0851 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Cancer Immunology, Immunotherapy |
| spelling | doaj-art-d4d52382ef25483f8adc464182717bf52025-02-09T12:39:13ZengSpringerCancer Immunology, Immunotherapy1432-08512025-02-0174311810.1007/s00262-025-03944-1Emerging insights into intravital imaging, unraveling its role in cancer immunotherapyMinfeng Yang0Shiqiang Hou1Yao Chen2Hongzhao Chen3Minjie Chu4Song-Bai Liu5School of Public Health, Nantong UniversityThe First People’s Hospital of Chuzhou, The Affiliated Chuzhou Hospital of Anhui Medical UniversityJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health CollegeDepartment of Health Technology and Informatics, The Hong Kong Polytechnic UniversitySchool of Public Health, Nantong UniversityJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in Oncology, Suzhou Vocational Health CollegeAbstract Cancer immunotherapy has attracted great attention as a potential therapeutic approach for advanced malignancies due to its promising survival benefits. Comprehension of intricate interactions between the tumor microenvironment (TME) and immune checkpoint inhibitors (ICIs) is crucial for optimizing and improving immunotherapies. Currently, several experimental strategies are available to monitor this complexity but most of them fail to facilitate real-time monitoring of the immune response such as cellular phagocytosis and cytolysis. Consequently, the application of intravital imaging has been extensively studied in the domain of cancer immunotherapy. Intravital imaging has been proven to be a powerful real-time imaging modality that provides insights into intratumoral immune responses, cellular metabolic signatures, tumor vasculature, and cellular functions. This review aims to provide a comprehensive overview of the latest research on intravital imaging in cancer immunotherapy, especially addressing how intravital imaging sheds light on essential features of tumor immunity, immune infiltrations, tumor angiogenesis, and aids in the clarification of underlying immunotherapeutic mechanisms. Moreover, a variety of labeling tools, imaging windows and models for real-time visualizations of TME are also summarized. We will also investigate the full potential of using intravital imaging to circumvent the limitations of currently available imaging modalities, which hold promise to advent efficient immunotherapy for cancer patients. Graphical abstracthttps://doi.org/10.1007/s00262-025-03944-1Intravital imagingImmunotherapyTumor angiogenesisTumor immunityImaging windows |
| spellingShingle | Minfeng Yang Shiqiang Hou Yao Chen Hongzhao Chen Minjie Chu Song-Bai Liu Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy Cancer Immunology, Immunotherapy Intravital imaging Immunotherapy Tumor angiogenesis Tumor immunity Imaging windows |
| title | Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy |
| title_full | Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy |
| title_fullStr | Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy |
| title_full_unstemmed | Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy |
| title_short | Emerging insights into intravital imaging, unraveling its role in cancer immunotherapy |
| title_sort | emerging insights into intravital imaging unraveling its role in cancer immunotherapy |
| topic | Intravital imaging Immunotherapy Tumor angiogenesis Tumor immunity Imaging windows |
| url | https://doi.org/10.1007/s00262-025-03944-1 |
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