Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression
Ethnopharmacological studies revealed that the leaves and stems of Mimosa pudica L. (Fabaceae) are widely used for the treatment of epilepsy. This study sought to investigate the effects of the aqueous extract of Mimosa pudica leaves and stems against pilocarpine–picrotoxin kindling-induced temporal...
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Frontiers Media S.A.
2025-02-01
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| author | Hart Mann Alain Youbi Mambou Simon Pale Orelien Sylvain Mtopi Bopda Vanessa Tita Jugha Nji Seraphin Ombel Musa Tambong Ako Ojongnkpot Bertrand Yuwong Wanyu Raymond Bess Bila Rashed N. Herqash Abdelaaty A. Shahat Germain Sotoing Taiwe |
| author_facet | Hart Mann Alain Youbi Mambou Simon Pale Orelien Sylvain Mtopi Bopda Vanessa Tita Jugha Nji Seraphin Ombel Musa Tambong Ako Ojongnkpot Bertrand Yuwong Wanyu Raymond Bess Bila Rashed N. Herqash Abdelaaty A. Shahat Germain Sotoing Taiwe |
| author_sort | Hart Mann Alain Youbi Mambou |
| collection | DOAJ |
| description | Ethnopharmacological studies revealed that the leaves and stems of Mimosa pudica L. (Fabaceae) are widely used for the treatment of epilepsy. This study sought to investigate the effects of the aqueous extract of Mimosa pudica leaves and stems against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy in mice and its implication on oxidative/nitrosative stress, GABAergic/cholinergic signalling, and brain-derived neurotrophic factor (BDNF) expression. The animals were treated for seven consecutive days as follows: one normal group and one negative control group that received orally distilled water; four test groups that received orally four doses of Mimosa pudica (20, 40, 80, and 160 mg/kg), respectively; and one positive control group that received 300 mg/kg sodium valproate intraperitoneally. One hour after the first treatment (first day), status epilepticus was induced by intraperitoneal injection of a single dose of pilocarpine (360 mg/kg). Then, 23 hours after the injection of pilocarpine to the mice, once again, they received their different treatments. Sixty minutes later, they were injected with a sub-convulsive dose of picrotoxin (1 mg/kg), and the anticonvulsant property of the extract was determined. On day 7, open-field, rotarod, and catalepsy tests were performed. Finally, the mice were sacrificed, and the hippocampi were isolated to quantify some biochemical markers of oxidative/nitrosative stress, GABAergic/cholinergic signalling, and BDNF levels in the hippocampus. Mimosa pudica extracts (160 mg/kg) significantly increased the latency time to status epilepticus by 70.91%. It significantly decreased the number of clonic and tonic seizures to 9.33 ± 1.03 and 5.00 ± 0.89, and their duration to 11.50 ± 2.07 and 6.83 ± 0.75 s, respectively. Exploratory behaviour, motor coordination, and catalepsy were significantly ameliorated, respectively, in the open-field, rotarod, and catalepsy tests. Pilocarpine–picrotoxin-induced alteration of oxidant–antioxidant balance, GABA-transaminase stability, acetylcholinesterase/butyrylcholinesterase activity, and neurogenesis were attenuated by the extract (80–160 mg/kg). This study showed that the aqueous extract of Mimosa pudica leaves and stems ameliorated epileptogenesis of temporal lobe epilepsy and could be used for the treatment of temporal lobe epilepsy. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-d8b5da72340a4874a7c3af8152fa3b602025-02-10T11:56:07ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-02-011510.3389/fphar.2024.13010021301002Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expressionHart Mann Alain Youbi Mambou0Simon Pale1Orelien Sylvain Mtopi Bopda2Vanessa Tita Jugha3Nji Seraphin Ombel Musa4Tambong Ako Ojongnkpot5Bertrand Yuwong Wanyu6Raymond Bess Bila7Rashed N. Herqash8Abdelaaty A. Shahat9Germain Sotoing Taiwe10Department of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Animal Biology and Conservation, Faculty of Science, University of Buea, Buea, CameroonEthnopharmacological studies revealed that the leaves and stems of Mimosa pudica L. (Fabaceae) are widely used for the treatment of epilepsy. This study sought to investigate the effects of the aqueous extract of Mimosa pudica leaves and stems against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy in mice and its implication on oxidative/nitrosative stress, GABAergic/cholinergic signalling, and brain-derived neurotrophic factor (BDNF) expression. The animals were treated for seven consecutive days as follows: one normal group and one negative control group that received orally distilled water; four test groups that received orally four doses of Mimosa pudica (20, 40, 80, and 160 mg/kg), respectively; and one positive control group that received 300 mg/kg sodium valproate intraperitoneally. One hour after the first treatment (first day), status epilepticus was induced by intraperitoneal injection of a single dose of pilocarpine (360 mg/kg). Then, 23 hours after the injection of pilocarpine to the mice, once again, they received their different treatments. Sixty minutes later, they were injected with a sub-convulsive dose of picrotoxin (1 mg/kg), and the anticonvulsant property of the extract was determined. On day 7, open-field, rotarod, and catalepsy tests were performed. Finally, the mice were sacrificed, and the hippocampi were isolated to quantify some biochemical markers of oxidative/nitrosative stress, GABAergic/cholinergic signalling, and BDNF levels in the hippocampus. Mimosa pudica extracts (160 mg/kg) significantly increased the latency time to status epilepticus by 70.91%. It significantly decreased the number of clonic and tonic seizures to 9.33 ± 1.03 and 5.00 ± 0.89, and their duration to 11.50 ± 2.07 and 6.83 ± 0.75 s, respectively. Exploratory behaviour, motor coordination, and catalepsy were significantly ameliorated, respectively, in the open-field, rotarod, and catalepsy tests. Pilocarpine–picrotoxin-induced alteration of oxidant–antioxidant balance, GABA-transaminase stability, acetylcholinesterase/butyrylcholinesterase activity, and neurogenesis were attenuated by the extract (80–160 mg/kg). This study showed that the aqueous extract of Mimosa pudica leaves and stems ameliorated epileptogenesis of temporal lobe epilepsy and could be used for the treatment of temporal lobe epilepsy.https://www.frontiersin.org/articles/10.3389/fphar.2024.1301002/fullMimosa pudicatemporal lobe epilepsyoxidative stressGABAergic statuscholinergic statusbrain-derived neurotrophic factors |
| spellingShingle | Hart Mann Alain Youbi Mambou Simon Pale Orelien Sylvain Mtopi Bopda Vanessa Tita Jugha Nji Seraphin Ombel Musa Tambong Ako Ojongnkpot Bertrand Yuwong Wanyu Raymond Bess Bila Rashed N. Herqash Abdelaaty A. Shahat Germain Sotoing Taiwe Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression Frontiers in Pharmacology Mimosa pudica temporal lobe epilepsy oxidative stress GABAergic status cholinergic status brain-derived neurotrophic factors |
| title | Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression |
| title_full | Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression |
| title_fullStr | Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression |
| title_full_unstemmed | Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression |
| title_short | Mimosapudica L. aqueous extract protects mice against pilocarpine–picrotoxin kindling-induced temporal lobe epilepsy, oxidative stress, and alteration in GABAergic/cholinergic pathways and BDNF expression |
| title_sort | mimosapudica l aqueous extract protects mice against pilocarpine picrotoxin kindling induced temporal lobe epilepsy oxidative stress and alteration in gabaergic cholinergic pathways and bdnf expression |
| topic | Mimosa pudica temporal lobe epilepsy oxidative stress GABAergic status cholinergic status brain-derived neurotrophic factors |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1301002/full |
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