An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC)
Abstract An exhaled breath sampler based on condensational growth and cyclone centrifugation (BSCC) was developed and evaluated. The BSCC increases the size of exhaled breath aerosols through condensational growth and then collects them as liquid sample via centrifugation. This enables rapid sample...
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| Format: | Article |
| Language: | English |
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Springer
2022-04-01
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| Series: | Aerosol and Air Quality Research |
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| Online Access: | https://doi.org/10.4209/aaqr.220049 |
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| author | Lebing Wang Jianguo Deng Dongbin Wang Menghao Chen Xue Li Yun Lu Jingkun Jiang |
| author_facet | Lebing Wang Jianguo Deng Dongbin Wang Menghao Chen Xue Li Yun Lu Jingkun Jiang |
| author_sort | Lebing Wang |
| collection | DOAJ |
| description | Abstract An exhaled breath sampler based on condensational growth and cyclone centrifugation (BSCC) was developed and evaluated. The BSCC increases the size of exhaled breath aerosols through condensational growth and then collects them as liquid sample via centrifugation. This enables rapid sample collection and eliminates certain pre-treatment steps for pathogenic microorganism analysis. Laboratory-generated aerosols were mixed with saturated water vapor to simulate exhaled breath, and the collection efficiency and the virus infectivity conservation efficiency of the BSCC were evaluated. The collection efficiency of the BSCC was approximately 66.7% for 100 nm aerosols and increased to nearly 100% for 3 µm aerosols. Besides, the BSCC maintained approximately 93.5% infectivity of atomized model virus aerosol (Pseudomonas bacteriophage Phi6). When collecting exhaled breath samples from nine volunteers, the average collection rate was 248.7 µL min−1. The BSCC achieved superior overall performance (i.e., 60% high collection efficiency and 40% higher infectivity conservation efficiency) compared with RTube, a commercial used exhaled breath sampler, indicating its potential for diagnosis of respiratory infection and measurements of exhaled viral aerosols. |
| format | Article |
| id | doaj-art-d9f64e252db943c78c5660b83d3e7e0d |
| institution | Kabale University |
| issn | 1680-8584 2071-1409 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Springer |
| record_format | Article |
| series | Aerosol and Air Quality Research |
| spelling | doaj-art-d9f64e252db943c78c5660b83d3e7e0d2025-02-09T12:17:22ZengSpringerAerosol and Air Quality Research1680-85842071-14092022-04-0122611010.4209/aaqr.220049An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC)Lebing Wang0Jianguo Deng1Dongbin Wang2Menghao Chen3Xue Li4Yun Lu5Jingkun Jiang6State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityState Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua UniversityAbstract An exhaled breath sampler based on condensational growth and cyclone centrifugation (BSCC) was developed and evaluated. The BSCC increases the size of exhaled breath aerosols through condensational growth and then collects them as liquid sample via centrifugation. This enables rapid sample collection and eliminates certain pre-treatment steps for pathogenic microorganism analysis. Laboratory-generated aerosols were mixed with saturated water vapor to simulate exhaled breath, and the collection efficiency and the virus infectivity conservation efficiency of the BSCC were evaluated. The collection efficiency of the BSCC was approximately 66.7% for 100 nm aerosols and increased to nearly 100% for 3 µm aerosols. Besides, the BSCC maintained approximately 93.5% infectivity of atomized model virus aerosol (Pseudomonas bacteriophage Phi6). When collecting exhaled breath samples from nine volunteers, the average collection rate was 248.7 µL min−1. The BSCC achieved superior overall performance (i.e., 60% high collection efficiency and 40% higher infectivity conservation efficiency) compared with RTube, a commercial used exhaled breath sampler, indicating its potential for diagnosis of respiratory infection and measurements of exhaled viral aerosols.https://doi.org/10.4209/aaqr.220049Viral aerosol collectionExhaled breathSampler developmentCollection efficiencyViral infectivity conservation |
| spellingShingle | Lebing Wang Jianguo Deng Dongbin Wang Menghao Chen Xue Li Yun Lu Jingkun Jiang An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC) Aerosol and Air Quality Research Viral aerosol collection Exhaled breath Sampler development Collection efficiency Viral infectivity conservation |
| title | An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC) |
| title_full | An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC) |
| title_fullStr | An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC) |
| title_full_unstemmed | An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC) |
| title_short | An Exhaled Breath Sampler Based on Condensational Growth and Cyclone Centrifugation (BSCC) |
| title_sort | exhaled breath sampler based on condensational growth and cyclone centrifugation bscc |
| topic | Viral aerosol collection Exhaled breath Sampler development Collection efficiency Viral infectivity conservation |
| url | https://doi.org/10.4209/aaqr.220049 |
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