Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro
The objective of this study is to investigate the effects of n-butylboronic acid (n-BA) on the proliferative activity, alkaline phosphatase activity, expression of hard tissue formation related genes, ALP and GJA1, and boron transporter gene, NaBC1, in human dental pulp cells. Human dental pulp cell...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Dental Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fdmed.2025.1518389/full |
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| author | Takashi Nakano Hidenori Hamba Takashi Muramatsu |
| author_facet | Takashi Nakano Hidenori Hamba Takashi Muramatsu |
| author_sort | Takashi Nakano |
| collection | DOAJ |
| description | The objective of this study is to investigate the effects of n-butylboronic acid (n-BA) on the proliferative activity, alkaline phosphatase activity, expression of hard tissue formation related genes, ALP and GJA1, and boron transporter gene, NaBC1, in human dental pulp cells. Human dental pulp cells were spread in culture medium with n-BA. The proliferative activity was increased in n-BA group. alkaline phosphatase staining and alizarin red S staining identified no obvious differences between the n-BA(+) and n-BA(−) groups. Alkaline phosphatase activity was significantly increased in the n-BA(+) group compared to the n-BA(−) group. Expressions of GJA1, ALP, and NaBC1 in the n-BA(+) group were higher than that of the n-BA(−) group. There were no significant differences between NaBC1-shRNA-transfected group and control group about proliferation and alkaline phosphatase activity. The results suggest that n-BA promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1, in human dental pulp cells. |
| format | Article |
| id | doaj-art-dc276593e0024ff387e66ba893592351 |
| institution | Kabale University |
| issn | 2673-4915 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Dental Medicine |
| spelling | doaj-art-dc276593e0024ff387e66ba8935923512025-02-11T06:59:45ZengFrontiers Media S.A.Frontiers in Dental Medicine2673-49152025-02-01610.3389/fdmed.2025.15183891518389Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitroTakashi NakanoHidenori HambaTakashi MuramatsuThe objective of this study is to investigate the effects of n-butylboronic acid (n-BA) on the proliferative activity, alkaline phosphatase activity, expression of hard tissue formation related genes, ALP and GJA1, and boron transporter gene, NaBC1, in human dental pulp cells. Human dental pulp cells were spread in culture medium with n-BA. The proliferative activity was increased in n-BA group. alkaline phosphatase staining and alizarin red S staining identified no obvious differences between the n-BA(+) and n-BA(−) groups. Alkaline phosphatase activity was significantly increased in the n-BA(+) group compared to the n-BA(−) group. Expressions of GJA1, ALP, and NaBC1 in the n-BA(+) group were higher than that of the n-BA(−) group. There were no significant differences between NaBC1-shRNA-transfected group and control group about proliferation and alkaline phosphatase activity. The results suggest that n-BA promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1, in human dental pulp cells.https://www.frontiersin.org/articles/10.3389/fdmed.2025.1518389/fullboroncell differentiationcell proliferationdental pulp cellsresinGJA1 |
| spellingShingle | Takashi Nakano Hidenori Hamba Takashi Muramatsu Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro Frontiers in Dental Medicine boron cell differentiation cell proliferation dental pulp cells resin GJA1 |
| title | Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro |
| title_full | Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro |
| title_fullStr | Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro |
| title_full_unstemmed | Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro |
| title_short | Butylboronic acid promotes cell proliferation, alkaline phosphatase activity, and expression of GJA1, ALP, and NaBC1 in human dental pulp cells in vitro |
| title_sort | butylboronic acid promotes cell proliferation alkaline phosphatase activity and expression of gja1 alp and nabc1 in human dental pulp cells in vitro |
| topic | boron cell differentiation cell proliferation dental pulp cells resin GJA1 |
| url | https://www.frontiersin.org/articles/10.3389/fdmed.2025.1518389/full |
| work_keys_str_mv | AT takashinakano butylboronicacidpromotescellproliferationalkalinephosphataseactivityandexpressionofgja1alpandnabc1inhumandentalpulpcellsinvitro AT hidenorihamba butylboronicacidpromotescellproliferationalkalinephosphataseactivityandexpressionofgja1alpandnabc1inhumandentalpulpcellsinvitro AT takashimuramatsu butylboronicacidpromotescellproliferationalkalinephosphataseactivityandexpressionofgja1alpandnabc1inhumandentalpulpcellsinvitro |