Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition
Traditional medicine uses the roots and rhizomes of Polygonum bistorta L. (Polygonaceae) to treat cough, bronchitis, and other respiratory infections. Our goal was to gain insights into the lung protective effects of the roots of P. bistorta L. against lipopolysaccharide-induced acute lung injury in...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1500085/full |
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| author | Sajida Parveen Kashif ur Rehman Khan Shahid Muhammad Iqbal Hanan Y. Aati Areej M. Al-taweel Liaqat Hussain Musaddique Hussain Musaddique Hussain |
| author_facet | Sajida Parveen Kashif ur Rehman Khan Shahid Muhammad Iqbal Hanan Y. Aati Areej M. Al-taweel Liaqat Hussain Musaddique Hussain Musaddique Hussain |
| author_sort | Sajida Parveen |
| collection | DOAJ |
| description | Traditional medicine uses the roots and rhizomes of Polygonum bistorta L. (Polygonaceae) to treat cough, bronchitis, and other respiratory infections. Our goal was to gain insights into the lung protective effects of the roots of P. bistorta L. against lipopolysaccharide-induced acute lung injury in rats, along with the possible mechanism(s). The outcomes revealed deliberate quantities of the total phenolic and flavonoid contents of 156.2 ± 5.13 GAE/g and 179.45 ± 2.08 mg QE/g, respectively. Crude extract possesses a maximum inhibitory potential of 81.77% ± 0.62% for acetylcholinesterase against eserine. Acute oral toxicity study revealed LD50 beyond 7 g/kg. Plant extract markedly restored LPS-induced hypoxemia, pulmonary edema, histopathological alterations, and leukocyte infiltration in the lung. ELISA testing on BALF found that the plant extract efficiently reinstated superoxide dismutase, total anti-oxidant capacity, malondialdehyde, and total oxidative stress. qRT-PCR indicated a decline in the endotoxin-induced overproduction of pro-inflammatory markers, oxidative stress, transcription factor, and downregulated antioxidant potential in extract-treated groups. Furthermore, 24 metabolites were identified and quantified via GC-MS. A molecular docking procedure was implemented on the bioactive metabolites that were identified to evaluate their potential for inhibiting AChE. In conclusion, P. bistorta roots mitigate inflammation and oxidative stress by improving redox signaling and NF-ĸβ (p65) pathways and can thus play a role in strategies for overcoming therapeutic challenges. |
| format | Article |
| id | doaj-art-dc95d6d24dbd4c8ba9487c0598de844e |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-dc95d6d24dbd4c8ba9487c0598de844e2025-02-11T09:22:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-02-011510.3389/fphar.2024.15000851500085Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibitionSajida Parveen0Kashif ur Rehman Khan1Shahid Muhammad Iqbal2Hanan Y. Aati3Areej M. Al-taweel4Liaqat Hussain5Musaddique Hussain6Musaddique Hussain7Department of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, PakistanMichael Sars Center, University of Bergen, Bergen, NorwayDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, PakistanDepartment of Pharmacology, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, PakistanDepartment of Medicine, University of Alabama at Birmingham, Birmingham, AL, United StatesTraditional medicine uses the roots and rhizomes of Polygonum bistorta L. (Polygonaceae) to treat cough, bronchitis, and other respiratory infections. Our goal was to gain insights into the lung protective effects of the roots of P. bistorta L. against lipopolysaccharide-induced acute lung injury in rats, along with the possible mechanism(s). The outcomes revealed deliberate quantities of the total phenolic and flavonoid contents of 156.2 ± 5.13 GAE/g and 179.45 ± 2.08 mg QE/g, respectively. Crude extract possesses a maximum inhibitory potential of 81.77% ± 0.62% for acetylcholinesterase against eserine. Acute oral toxicity study revealed LD50 beyond 7 g/kg. Plant extract markedly restored LPS-induced hypoxemia, pulmonary edema, histopathological alterations, and leukocyte infiltration in the lung. ELISA testing on BALF found that the plant extract efficiently reinstated superoxide dismutase, total anti-oxidant capacity, malondialdehyde, and total oxidative stress. qRT-PCR indicated a decline in the endotoxin-induced overproduction of pro-inflammatory markers, oxidative stress, transcription factor, and downregulated antioxidant potential in extract-treated groups. Furthermore, 24 metabolites were identified and quantified via GC-MS. A molecular docking procedure was implemented on the bioactive metabolites that were identified to evaluate their potential for inhibiting AChE. In conclusion, P. bistorta roots mitigate inflammation and oxidative stress by improving redox signaling and NF-ĸβ (p65) pathways and can thus play a role in strategies for overcoming therapeutic challenges.https://www.frontiersin.org/articles/10.3389/fphar.2024.1500085/fullpolygonum bistortapolygonaceaeNF-ĸβgene expressionmedicinal plantsmolecular docking |
| spellingShingle | Sajida Parveen Kashif ur Rehman Khan Shahid Muhammad Iqbal Hanan Y. Aati Areej M. Al-taweel Liaqat Hussain Musaddique Hussain Musaddique Hussain Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition Frontiers in Pharmacology polygonum bistorta polygonaceae NF-ĸβ gene expression medicinal plants molecular docking |
| title | Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition |
| title_full | Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition |
| title_fullStr | Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition |
| title_full_unstemmed | Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition |
| title_short | Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition |
| title_sort | exploration of the anti inflammatory potential of polygonum bistorta l protection against lps induced acute lung injury in rats via nf qβ pathway inhibition |
| topic | polygonum bistorta polygonaceae NF-ĸβ gene expression medicinal plants molecular docking |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1500085/full |
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