Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology
This study evaluated the effects of calcium butyrate (CB) and bacitracin methylene disalicylate 50 (BMD) combined with different phytase concentrations on broiler intestinal health and physiology. Day-old YPM x Ross 708 male broilers (2,880) were distributed in 72 floor pens and assigned to 1 of 9 t...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000999 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864519866187776 |
|---|---|
| author | Joseph P. Gulizia Zubair Khalid Maria T. Terra-Long Jose I. Vargas Jose R. Hernandez Wilmer J. Pacheco James T. Krehling Kenneth S. Macklin William A. Dozier, III Klint W. McCafferty Ruediger Hauck |
| author_facet | Joseph P. Gulizia Zubair Khalid Maria T. Terra-Long Jose I. Vargas Jose R. Hernandez Wilmer J. Pacheco James T. Krehling Kenneth S. Macklin William A. Dozier, III Klint W. McCafferty Ruediger Hauck |
| author_sort | Joseph P. Gulizia |
| collection | DOAJ |
| description | This study evaluated the effects of calcium butyrate (CB) and bacitracin methylene disalicylate 50 (BMD) combined with different phytase concentrations on broiler intestinal health and physiology. Day-old YPM x Ross 708 male broilers (2,880) were distributed in 72 floor pens and assigned to 1 of 9 treatments (8 replicates/treatment). This experiment was a factorial arrangement including 2 phytase concentrations (500 or 1,500 FTU/kg) and 4 microbiota modulating feed additive levels (MMFA; (1) none, (2) only CB (0.5 g/kg of diet), (3) only BMD (55 mg/kg of diet), or (4) both CB and BMD). Additionally, a negative control without phytase and MMFA was included. Intestinal permeability was assessed on d 27. Jejunum wall and cecal content samples were collected on d 28 and 42 to assess jejunum villus height (VH), crypt depth, tight-junction and mucin gene expression, cecal microbiome diversity, and predicted bacterial metabolic pathways. Phytase and MMFA did not influence intestinal permeability (P > 0.05). Combining both CB and BMD with 1,500 FTU/kg of phytase compared to 500 FTU/kg lowered d 28 VH (P ≤ 0.05). Jejunal expression of CL-1, CL-4, CL-5, and ZO-2 on d 28 as well as CL-2 on d 42 changed between MMFA when combined with 1,500 FTU/kg of phytase but not 500 FTU/kg (P ≤ 0.05). Day 42 Pielou's evenness increased when 1,500 FTU/kg of phytase was combined with both CB and BMD compared to no MMFA (P ≤ 0.05). The cecal microbial beta diversity was not influenced by phytase, MMFA, or their interaction (P > 0.05). Overall, broiler intestinal health and physiology were influenced by CB and BMD depending on phytase concentration, demonstrating the complex interactions between these feed additives. |
| format | Article |
| id | doaj-art-de2514ee8b0048b0af749d0baff5af2d |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-de2514ee8b0048b0af749d0baff5af2d2025-02-09T04:59:34ZengElsevierPoultry Science0032-57912025-03-011043104862Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiologyJoseph P. Gulizia0Zubair Khalid1Maria T. Terra-Long2Jose I. Vargas3Jose R. Hernandez4Wilmer J. Pacheco5James T. Krehling6Kenneth S. Macklin7William A. Dozier, III8Klint W. McCafferty9Ruediger Hauck10Department of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Pathobiology, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United StatesUSDA-ARS Poultry Research Unit, Mississippi State, MS 39762, United StatesDepartment of Poultry Science, Auburn University, Auburn, AL 36849, United States; Department of Pathobiology, Auburn University, Auburn, AL 36849, United States; Corresponding author at: 260 Lem Morrison Dr., Auburn, AL 36849, United StatesThis study evaluated the effects of calcium butyrate (CB) and bacitracin methylene disalicylate 50 (BMD) combined with different phytase concentrations on broiler intestinal health and physiology. Day-old YPM x Ross 708 male broilers (2,880) were distributed in 72 floor pens and assigned to 1 of 9 treatments (8 replicates/treatment). This experiment was a factorial arrangement including 2 phytase concentrations (500 or 1,500 FTU/kg) and 4 microbiota modulating feed additive levels (MMFA; (1) none, (2) only CB (0.5 g/kg of diet), (3) only BMD (55 mg/kg of diet), or (4) both CB and BMD). Additionally, a negative control without phytase and MMFA was included. Intestinal permeability was assessed on d 27. Jejunum wall and cecal content samples were collected on d 28 and 42 to assess jejunum villus height (VH), crypt depth, tight-junction and mucin gene expression, cecal microbiome diversity, and predicted bacterial metabolic pathways. Phytase and MMFA did not influence intestinal permeability (P > 0.05). Combining both CB and BMD with 1,500 FTU/kg of phytase compared to 500 FTU/kg lowered d 28 VH (P ≤ 0.05). Jejunal expression of CL-1, CL-4, CL-5, and ZO-2 on d 28 as well as CL-2 on d 42 changed between MMFA when combined with 1,500 FTU/kg of phytase but not 500 FTU/kg (P ≤ 0.05). Day 42 Pielou's evenness increased when 1,500 FTU/kg of phytase was combined with both CB and BMD compared to no MMFA (P ≤ 0.05). The cecal microbial beta diversity was not influenced by phytase, MMFA, or their interaction (P > 0.05). Overall, broiler intestinal health and physiology were influenced by CB and BMD depending on phytase concentration, demonstrating the complex interactions between these feed additives.http://www.sciencedirect.com/science/article/pii/S0032579125000999PhytaseCalcium butyrateBacitracin methylene disalicylateBroilerHealth |
| spellingShingle | Joseph P. Gulizia Zubair Khalid Maria T. Terra-Long Jose I. Vargas Jose R. Hernandez Wilmer J. Pacheco James T. Krehling Kenneth S. Macklin William A. Dozier, III Klint W. McCafferty Ruediger Hauck Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology Poultry Science Phytase Calcium butyrate Bacitracin methylene disalicylate Broiler Health |
| title | Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology |
| title_full | Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology |
| title_fullStr | Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology |
| title_full_unstemmed | Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology |
| title_short | Response of YPM x Ross 708 male broilers to diets containing varying inclusions of phytase, calcium butyrate, and bacitracin methylene disalicylate during the grower and finisher periods–part 2: Intestinal health and physiology |
| title_sort | response of ypm x ross 708 male broilers to diets containing varying inclusions of phytase calcium butyrate and bacitracin methylene disalicylate during the grower and finisher periods part 2 intestinal health and physiology |
| topic | Phytase Calcium butyrate Bacitracin methylene disalicylate Broiler Health |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000999 |
| work_keys_str_mv | AT josephpgulizia responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT zubairkhalid responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT mariatterralong responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT joseivargas responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT joserhernandez responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT wilmerjpacheco responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT jamestkrehling responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT kennethsmacklin responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT williamadozieriii responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT klintwmccafferty responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology AT ruedigerhauck responseofypmxross708malebroilerstodietscontainingvaryinginclusionsofphytasecalciumbutyrateandbacitracinmethylenedisalicylateduringthegrowerandfinisherperiodspart2intestinalhealthandphysiology |