Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum
Abstract Background The efficacy of biocontrol agents depends critically on their environmental adaptability, with nutrient availability being a key determinant of their success. In Trichoderma species, the mechanisms linking nutrient sensing to physiological responses remain poorly understood, desp...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-02-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-025-00735-9 |
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| author | Stefania Vitale Felice Salzano Alessia Staropoli Roberta Marra David Turrà Matteo Lorito Francesco Vinale |
| author_facet | Stefania Vitale Felice Salzano Alessia Staropoli Roberta Marra David Turrà Matteo Lorito Francesco Vinale |
| author_sort | Stefania Vitale |
| collection | DOAJ |
| description | Abstract Background The efficacy of biocontrol agents depends critically on their environmental adaptability, with nutrient availability being a key determinant of their success. In Trichoderma species, the mechanisms linking nutrient sensing to physiological responses remain poorly understood, despite their importance for biocontrol applications. Results This study reveals how different nitrogen sources fundamentally regulate the physiology and metabolism of Trichoderma harzianum through pH modulation. Under nutrient-rich conditions, T. harzianum exhibited a biphasic pH response characterized by initial acidification followed by alkalinization, which correlated with enhanced sporulation. Examining specific nitrogen sources, we found that sodium nitrate induced environmental alkalinization, while ammonium nitrate caused sustained acidification. These pH changes were linked to distinct physiological responses: alkaline conditions promoted sporulation, while acidic conditions enhanced mycelial growth and triggered specific metabolic responses. Notably, acidic conditions specifically induced the production of harzianic acid and related bioactive compounds, suggesting pH-dependent regulation of secondary metabolism. This nitrogen-dependent pH modulation pattern was conserved across Trichoderma species, as demonstrated by parallel responses in T. asperellum. Conclusions Our findings establish nitrogen source availability as a master regulator of Trichoderma physiology through pH-dependent mechanisms, controlling both development and secondary metabolism. This understanding provides new strategies for optimizing biocontrol formulations by manipulating nitrogen sources and pH conditions to enhance both fungal fitness and beneficial metabolite production. Graphical Abstract |
| format | Article |
| id | doaj-art-de9a976a3f444ebfa76eaecd6e733acf |
| institution | Kabale University |
| issn | 2196-5641 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj-art-de9a976a3f444ebfa76eaecd6e733acf2025-02-09T12:16:15ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412025-02-0112111510.1186/s40538-025-00735-9Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianumStefania Vitale0Felice Salzano1Alessia Staropoli2Roberta Marra3David Turrà4Matteo Lorito5Francesco Vinale6Department of Agricultural Sciences, University of Naples Federico IIDepartment of Agricultural Sciences, University of Naples Federico IIDepartment of Agricultural Sciences, University of Naples Federico IIDepartment of Agricultural Sciences, University of Naples Federico IIDepartment of Agricultural Sciences, University of Naples Federico IIDepartment of Agricultural Sciences, University of Naples Federico IIDepartment of Veterinary Medicine and Animal Production, University of Naples Federico IIAbstract Background The efficacy of biocontrol agents depends critically on their environmental adaptability, with nutrient availability being a key determinant of their success. In Trichoderma species, the mechanisms linking nutrient sensing to physiological responses remain poorly understood, despite their importance for biocontrol applications. Results This study reveals how different nitrogen sources fundamentally regulate the physiology and metabolism of Trichoderma harzianum through pH modulation. Under nutrient-rich conditions, T. harzianum exhibited a biphasic pH response characterized by initial acidification followed by alkalinization, which correlated with enhanced sporulation. Examining specific nitrogen sources, we found that sodium nitrate induced environmental alkalinization, while ammonium nitrate caused sustained acidification. These pH changes were linked to distinct physiological responses: alkaline conditions promoted sporulation, while acidic conditions enhanced mycelial growth and triggered specific metabolic responses. Notably, acidic conditions specifically induced the production of harzianic acid and related bioactive compounds, suggesting pH-dependent regulation of secondary metabolism. This nitrogen-dependent pH modulation pattern was conserved across Trichoderma species, as demonstrated by parallel responses in T. asperellum. Conclusions Our findings establish nitrogen source availability as a master regulator of Trichoderma physiology through pH-dependent mechanisms, controlling both development and secondary metabolism. This understanding provides new strategies for optimizing biocontrol formulations by manipulating nitrogen sources and pH conditions to enhance both fungal fitness and beneficial metabolite production. Graphical Abstracthttps://doi.org/10.1186/s40538-025-00735-9Trichoderma spp.Nitrogen sourcesExtracellular pHSporulationVegetative growthHarzianic acid |
| spellingShingle | Stefania Vitale Felice Salzano Alessia Staropoli Roberta Marra David Turrà Matteo Lorito Francesco Vinale Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum Chemical and Biological Technologies in Agriculture Trichoderma spp. Nitrogen sources Extracellular pH Sporulation Vegetative growth Harzianic acid |
| title | Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum |
| title_full | Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum |
| title_fullStr | Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum |
| title_full_unstemmed | Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum |
| title_short | Nitrogen source orchestrates pH modulation and secondary metabolism in Trichoderma harzianum |
| title_sort | nitrogen source orchestrates ph modulation and secondary metabolism in trichoderma harzianum |
| topic | Trichoderma spp. Nitrogen sources Extracellular pH Sporulation Vegetative growth Harzianic acid |
| url | https://doi.org/10.1186/s40538-025-00735-9 |
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