Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus
Staphylococcus aureus (S. aureus) is a major foodborne pathogen, could lead cause of intestinal infections in humans. Antimicrobial peptides, as emerging antimicrobial agents, are gradually replacing traditional agents due to their highly effective and safe antimicrobial activity. In this study, a n...
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Elsevier
2025-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927125000292 |
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| author | Ritian Jin Guanglei Wei Rong Lin Wenfeng Lin Jude Juventus Aweya Duo Liang Wuyin Weng Shen Yang |
| author_facet | Ritian Jin Guanglei Wei Rong Lin Wenfeng Lin Jude Juventus Aweya Duo Liang Wuyin Weng Shen Yang |
| author_sort | Ritian Jin |
| collection | DOAJ |
| description | Staphylococcus aureus (S. aureus) is a major foodborne pathogen, could lead cause of intestinal infections in humans. Antimicrobial peptides, as emerging antimicrobial agents, are gradually replacing traditional agents due to their highly effective and safe antimicrobial activity. In this study, a novel peptide, designated as FAM286, was identified from TASOR protein of Larimichthys crocea, which had a strong antimicrobial activity against S. aureus with a minimum bactericidal concentration (MBC) of 3.9 μg/mL and completed bacteria killing by treatment for 1.5 h. The FAM286 could increase the permeability and disrupt the integrity of cell membranes. The cell showed aggregation phenomenon and entered the apoptosis stage. In addition, the non-bactericidal concentration of FAM286 could effectively inhibit the formation of biofilm and remove mature biofilm. Molecular docking experiments further verified the binding sites of FAM286 to S. aureus biofilm proteins SarA, AgrA, and Hld. FAM286 could also bind the bacterial DNA in an embedded manner, disrupting the structure of DNA and leading to the death of bacteria. This study comprehensively evaluated the antimicrobial mechanism of the FAM286 against S. aureus and provided a theoretical basis for the prevention and control of S. aureus. |
| format | Article |
| id | doaj-art-df41f1ae90da499887b9f2362bf84005 |
| institution | Kabale University |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-df41f1ae90da499887b9f2362bf840052025-02-08T05:01:09ZengElsevierCurrent Research in Food Science2665-92712025-01-0110100998Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureusRitian Jin0Guanglei Wei1Rong Lin2Wenfeng Lin3Jude Juventus Aweya4Duo Liang5Wuyin Weng6Shen Yang7College of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaCorresponding author. College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian, 361021, China.; College of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian, 361021, ChinaStaphylococcus aureus (S. aureus) is a major foodborne pathogen, could lead cause of intestinal infections in humans. Antimicrobial peptides, as emerging antimicrobial agents, are gradually replacing traditional agents due to their highly effective and safe antimicrobial activity. In this study, a novel peptide, designated as FAM286, was identified from TASOR protein of Larimichthys crocea, which had a strong antimicrobial activity against S. aureus with a minimum bactericidal concentration (MBC) of 3.9 μg/mL and completed bacteria killing by treatment for 1.5 h. The FAM286 could increase the permeability and disrupt the integrity of cell membranes. The cell showed aggregation phenomenon and entered the apoptosis stage. In addition, the non-bactericidal concentration of FAM286 could effectively inhibit the formation of biofilm and remove mature biofilm. Molecular docking experiments further verified the binding sites of FAM286 to S. aureus biofilm proteins SarA, AgrA, and Hld. FAM286 could also bind the bacterial DNA in an embedded manner, disrupting the structure of DNA and leading to the death of bacteria. This study comprehensively evaluated the antimicrobial mechanism of the FAM286 against S. aureus and provided a theoretical basis for the prevention and control of S. aureus.http://www.sciencedirect.com/science/article/pii/S2665927125000292Staphylococcus aureusAntimicrobial peptideLarimichthys croceaTASOR proteinAntimicrobial mechanism |
| spellingShingle | Ritian Jin Guanglei Wei Rong Lin Wenfeng Lin Jude Juventus Aweya Duo Liang Wuyin Weng Shen Yang Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus Current Research in Food Science Staphylococcus aureus Antimicrobial peptide Larimichthys crocea TASOR protein Antimicrobial mechanism |
| title | Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus |
| title_full | Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus |
| title_fullStr | Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus |
| title_full_unstemmed | Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus |
| title_short | Efficacy of Larimichthys crocea TASOR protein-derived peptide FAM286 against Staphylococcus aureus |
| title_sort | efficacy of larimichthys crocea tasor protein derived peptide fam286 against staphylococcus aureus |
| topic | Staphylococcus aureus Antimicrobial peptide Larimichthys crocea TASOR protein Antimicrobial mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S2665927125000292 |
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