Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes
Background The widespread dissemination of carbapenem-resistant gram-negative bacteria poses a significant threat to global public health. Purpose This study aimed to investigate the prevalence of carbapenem resistance in gram-negative bacteria isolated from patients at the Children's Medical C...
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| Format: | Article |
| Language: | English |
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The Korean Pediatric Society
2025-01-01
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| Series: | Clinical and Experimental Pediatrics |
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| Online Access: | http://www.e-cep.org/upload/pdf/cep-2023-01774.pdf |
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| author | Setareh Mamishi Reihaneh Hosseinpour Sadeghi Sadaf Sajedi Moghaddam Babak Pourakbari Shiva Poormohammadi Maryam Sotoudeh Anvari Shima Mahmoudi |
| author_facet | Setareh Mamishi Reihaneh Hosseinpour Sadeghi Sadaf Sajedi Moghaddam Babak Pourakbari Shiva Poormohammadi Maryam Sotoudeh Anvari Shima Mahmoudi |
| author_sort | Setareh Mamishi |
| collection | DOAJ |
| description | Background The widespread dissemination of carbapenem-resistant gram-negative bacteria poses a significant threat to global public health. Purpose This study aimed to investigate the prevalence of carbapenem resistance in gram-negative bacteria isolated from patients at the Children's Medical Center Hospital, Tehran, Iran, to understand the molecular mechanisms underlying this resistance. Methods During the period spanning from June 2019 to June 2020, 777 gram-negative bacterial strains were isolated. Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standards Institute. Polymerase chain reaction was used to detect carbapenem resistance genes including bla OXA23, bla OXA24, bla OXA48, bla OXA51, bla OXA58, bla OXA143, bla KPC, bla IMP, bla VIM, and bla NDM. Results Among the total bacterial isolates, 141 (18.1%) exhibited carbapenem resistance. Escherichia coli was the most prevalent (57.4%), followed by Klebsiella pneumoniae (11.3%), and Acinetobacter baumannii (10.6%). Other notable contributors included Enterobacter spp. (5.7%), Salmonella spp. (3.5%), and Stenotrophomonas maltophilia (2.8%). Citrobacter spp., Proteus mirabilis, and Pseudomonas aeruginosa contributed to the distributions of 2, 1, and 3 isolates, respectively. Notably, bla OXA48 showed the highest prevalence (33%), followed by bla OXA143 and bla OXA5 8 (27% and 24%, respectively). In addition, bla OXA24 was present in 11% of the total isolates, bla OXA23 in 10%, and bla NDM in 10%, whereas bla KPC, bla VIM, and bla IMP were not detected. Conclusion Our study highlights the prevalence of carbapenemase-producing gram-negative isolates among pediatric patients. Notable resistance patterns, especially in K. pneumoniae and E. coli, underline the urgent need for proactive interventions, including appropriate antibiotic prescription practices and strengthening of antibiotic stewardship programs. |
| format | Article |
| id | doaj-art-df74b306d389493d8d31bb3ff1ebcf13 |
| institution | Kabale University |
| issn | 2713-4148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | The Korean Pediatric Society |
| record_format | Article |
| series | Clinical and Experimental Pediatrics |
| spelling | doaj-art-df74b306d389493d8d31bb3ff1ebcf132025-02-07T07:38:34ZengThe Korean Pediatric SocietyClinical and Experimental Pediatrics2713-41482025-01-01681657210.3345/cep.2023.0177420125555728Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genesSetareh Mamishi0Reihaneh Hosseinpour Sadeghi1Sadaf Sajedi Moghaddam2Babak Pourakbari3Shiva Poormohammadi4Maryam Sotoudeh Anvari5Shima Mahmoudi6 Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran Molecular Pathology and Cytogenetics Division, Pathology Department, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran Biotechnology Centre, Silesian University of Technology, Gliwice, PolandBackground The widespread dissemination of carbapenem-resistant gram-negative bacteria poses a significant threat to global public health. Purpose This study aimed to investigate the prevalence of carbapenem resistance in gram-negative bacteria isolated from patients at the Children's Medical Center Hospital, Tehran, Iran, to understand the molecular mechanisms underlying this resistance. Methods During the period spanning from June 2019 to June 2020, 777 gram-negative bacterial strains were isolated. Antibiotic susceptibility testing was performed according to Clinical and Laboratory Standards Institute. Polymerase chain reaction was used to detect carbapenem resistance genes including bla OXA23, bla OXA24, bla OXA48, bla OXA51, bla OXA58, bla OXA143, bla KPC, bla IMP, bla VIM, and bla NDM. Results Among the total bacterial isolates, 141 (18.1%) exhibited carbapenem resistance. Escherichia coli was the most prevalent (57.4%), followed by Klebsiella pneumoniae (11.3%), and Acinetobacter baumannii (10.6%). Other notable contributors included Enterobacter spp. (5.7%), Salmonella spp. (3.5%), and Stenotrophomonas maltophilia (2.8%). Citrobacter spp., Proteus mirabilis, and Pseudomonas aeruginosa contributed to the distributions of 2, 1, and 3 isolates, respectively. Notably, bla OXA48 showed the highest prevalence (33%), followed by bla OXA143 and bla OXA5 8 (27% and 24%, respectively). In addition, bla OXA24 was present in 11% of the total isolates, bla OXA23 in 10%, and bla NDM in 10%, whereas bla KPC, bla VIM, and bla IMP were not detected. Conclusion Our study highlights the prevalence of carbapenemase-producing gram-negative isolates among pediatric patients. Notable resistance patterns, especially in K. pneumoniae and E. coli, underline the urgent need for proactive interventions, including appropriate antibiotic prescription practices and strengthening of antibiotic stewardship programs.http://www.e-cep.org/upload/pdf/cep-2023-01774.pdfcarbapenem-resistantgram-negative bacteriaoxa-type carbapenemasendmkpc |
| spellingShingle | Setareh Mamishi Reihaneh Hosseinpour Sadeghi Sadaf Sajedi Moghaddam Babak Pourakbari Shiva Poormohammadi Maryam Sotoudeh Anvari Shima Mahmoudi Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes Clinical and Experimental Pediatrics carbapenem-resistant gram-negative bacteria oxa-type carbapenemase ndm kpc |
| title | Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes |
| title_full | Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes |
| title_fullStr | Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes |
| title_full_unstemmed | Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes |
| title_short | Carbapenem resistance in gram-negative pathogens in an Iranian hospital: high prevalence of OXA-type carbapenemase genes |
| title_sort | carbapenem resistance in gram negative pathogens in an iranian hospital high prevalence of oxa type carbapenemase genes |
| topic | carbapenem-resistant gram-negative bacteria oxa-type carbapenemase ndm kpc |
| url | http://www.e-cep.org/upload/pdf/cep-2023-01774.pdf |
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