Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing
Background Human breast milk (HBM) is an important source of tolerogenic immune mediators that influence the infant immune system. HBM-derived immune components are affected by various factors; however, few studies have examined the relationship between parity and immune cell profiles of HBM. Purpos...
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| Language: | English |
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The Korean Pediatric Society
2025-02-01
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| Series: | Clinical and Experimental Pediatrics |
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| Online Access: | http://www.e-cep.org/upload/pdf/cep-2024-01585.pdf |
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| author | Dae Yong Yi Hong-Jai Park Min Sun Shin Hyoungsu Kim Sang Jin Lee Insoo Kang |
| author_facet | Dae Yong Yi Hong-Jai Park Min Sun Shin Hyoungsu Kim Sang Jin Lee Insoo Kang |
| author_sort | Dae Yong Yi |
| collection | DOAJ |
| description | Background Human breast milk (HBM) is an important source of tolerogenic immune mediators that influence the infant immune system. HBM-derived immune components are affected by various factors; however, few studies have examined the relationship between parity and immune cell profiles of HBM. Purpose This study aimed to clarify the effects of parity on HBM immune cell heterogeneity and gene expression by integrating and analyzing publicly available single-cell RNAsequencing (scRNA-seq) datasets. Methods We clarified the effects of parity on HBM immune cell heterogeneity and gene expression by integrating and analyzing publicly available scRNA-seq datasets. Results The proportion of innate immune cells was significantly higher in the primiparous versus multiparous group, whereas the proportion of adaptive immune cells was significantly higher in the multiparous group (P=0.021). The 2 immune clusters were reannotated and classified into monocyte, T/B cell, and CD45¯ groups. The proportions of monocytes and T/B cells were higher in the primiparous and multiparous groups, respectively. In a gene set enrichment analysis of monocytes, genes with a direct role in the infant immune system and immune response-related genes were more highly expressed in the primiparous group. Conclusion Our results support the parity-dependent differences in gene expression between innate and adaptive immune cells. |
| format | Article |
| id | doaj-art-e0bfff2d79dc4e44953d6affee5d4d40 |
| institution | Kabale University |
| issn | 2713-4148 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | The Korean Pediatric Society |
| record_format | Article |
| series | Clinical and Experimental Pediatrics |
| spelling | doaj-art-e0bfff2d79dc4e44953d6affee5d4d402025-02-07T07:38:39ZengThe Korean Pediatric SocietyClinical and Experimental Pediatrics2713-41482025-02-0168214115210.3345/cep.2024.0158520125555774Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencingDae Yong Yi0Hong-Jai Park1Min Sun Shin2Hyoungsu Kim3Sang Jin Lee4Insoo Kang5 Department of Pediatrics, Chung-Ang University Hospital, Seoul, Korea Department of Internal Medicine, Section of Rheumatology, Allergy & Immunology, Yale University School of Medicine, New Haven, CT, USA Department of Internal Medicine, Section of Rheumatology, Allergy & Immunology, Yale University School of Medicine, New Haven, CT, USA Department of Internal Medicine, Kangdong Sacred Heart Hospital, Hallym University School of Medicine, Seoul, Korea Department of Internal Medicine, Kyungpook National University School of Medicine, Daegu, Korea Department of Internal Medicine, Section of Rheumatology, Allergy & Immunology, Yale University School of Medicine, New Haven, CT, USABackground Human breast milk (HBM) is an important source of tolerogenic immune mediators that influence the infant immune system. HBM-derived immune components are affected by various factors; however, few studies have examined the relationship between parity and immune cell profiles of HBM. Purpose This study aimed to clarify the effects of parity on HBM immune cell heterogeneity and gene expression by integrating and analyzing publicly available single-cell RNAsequencing (scRNA-seq) datasets. Methods We clarified the effects of parity on HBM immune cell heterogeneity and gene expression by integrating and analyzing publicly available scRNA-seq datasets. Results The proportion of innate immune cells was significantly higher in the primiparous versus multiparous group, whereas the proportion of adaptive immune cells was significantly higher in the multiparous group (P=0.021). The 2 immune clusters were reannotated and classified into monocyte, T/B cell, and CD45¯ groups. The proportions of monocytes and T/B cells were higher in the primiparous and multiparous groups, respectively. In a gene set enrichment analysis of monocytes, genes with a direct role in the infant immune system and immune response-related genes were more highly expressed in the primiparous group. Conclusion Our results support the parity-dependent differences in gene expression between innate and adaptive immune cells.http://www.e-cep.org/upload/pdf/cep-2024-01585.pdfhuman milksingle-cell rna-seqparity |
| spellingShingle | Dae Yong Yi Hong-Jai Park Min Sun Shin Hyoungsu Kim Sang Jin Lee Insoo Kang Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing Clinical and Experimental Pediatrics human milk single-cell rna-seq parity |
| title | Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing |
| title_full | Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing |
| title_fullStr | Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing |
| title_full_unstemmed | Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing |
| title_short | Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing |
| title_sort | differences in immune cells and gene expression in human milk by parity on integrated scrna sequencing |
| topic | human milk single-cell rna-seq parity |
| url | http://www.e-cep.org/upload/pdf/cep-2024-01585.pdf |
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