Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria
OBJECTIVES: Spontaneous abortion (miscarriage) is a common adverse pregnancy outcome earth-wide, and has remained a challenge in Nigeria. This study aimed at comparing the vaginal microbiome of women who have had episodes of spontaneous abortion with those who have not experienced any incident - in...
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Medical Network
2023-03-01
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| Series: | Gynecology Obstetrics & Reproductive Medicine |
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| Online Access: | https://gorm.com.tr/index.php/GORM/article/view/1297 |
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| author | Felix Emele Emele Prisca Onyeulor Francisca Nwaokorie Damian Asogwa |
| author_facet | Felix Emele Emele Prisca Onyeulor Francisca Nwaokorie Damian Asogwa |
| author_sort | Felix Emele Emele |
| collection | DOAJ |
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OBJECTIVES: Spontaneous abortion (miscarriage) is a common adverse pregnancy outcome earth-wide, and has remained a challenge in Nigeria. This study aimed at comparing the vaginal microbiome of women who have had episodes of spontaneous abortion with those who have not experienced any incident - in order to find out any possible role of vaginal microbiota in spontaneous abortion.
STUDY DESIGN: High vaginal swab samples were collected from the vagina fornix of 6 women of reproductive age, with a history of recurrent spontaneous abortion, as well as those without such history (non-spontaneous abortion). The samples were analyzed and interpreted by standard metagenomic and bioinformatic techniques.
RESULTS: The following phyla were encountered in spontaneous abortion and non-spontaneous abortion, respectively: Firmicutes (69.4%, 94.9%), Actinobacteria (12.7%, 1.1%), Bacteroidetes (9.5%, 2.8%), Proteobacteria (7.9%, 0.3%), Chloroflexi (0.2%, 0.0%), Fusobacteria (0.2%, 0.0%), Tenericutes (0.02%, 1.0%). There was more bacterial diversity in spontaneous abortion (H=2.34856) than in Non-spontaneous abortion (H=0.61384), with evenness (EH) of 0.60668 and 0.24703, respectively. On the contrary, Lactobacillus had more relative abundance in non-spontaneous abortion (83%) than spontaneous abortion (23.5%). The following genera (among others) occurred exclusively in spontaneous abortion: Enterococcus (relative abundance=26%), Peptostreptococcus (5.1%), Anaerococcus (2.4%), Dialister (2.1%), Streptococcus (1.9%), Megasphaera (1.3%), Mobiluncus (1.0%), Peptinophilus (0.9%), and Veillonella (0.7%). The efficiency of taxonomic identification, using the operational taxonomic unit clustering method, declined, downstream, from family to species levels.
CONCLUSION: Recurrent spontaneous abortion appears to be associated with low vaginal Lactobacillus abundance and high bacterial diversity. We recommend that the current operational taxonomic unit -based sequence taxonomic analysis technique be reviewed.
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| format | Article |
| id | doaj-art-e229ffa84df547d5a588ab09f3761aad |
| institution | Kabale University |
| issn | 1300-4751 2602-4918 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Medical Network |
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| series | Gynecology Obstetrics & Reproductive Medicine |
| spelling | doaj-art-e229ffa84df547d5a588ab09f3761aad2025-02-11T21:16:47ZengMedical NetworkGynecology Obstetrics & Reproductive Medicine1300-47512602-49182023-03-0129110.21613/GORM.2022.1297Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern NigeriaFelix Emele Emele0Prisca Onyeulor1Francisca Nwaokorie2Damian Asogwa3Nnamdi Azikiwe University, Nnewi, NigeriaDepartment of Medical Microbiology and Parasitology, Faculty of Medicine, Nnamdi Azikiwe University, Nnewi, Nigeria.School of Medical Laboratory Sciences, University of Lagos, Lagos, Nigeria.Department of Obstatrics and Gynaecology, Alex Ekwueme Federal University Teaching Hospital, Abakaliki, Ebonyi State, Nigeria OBJECTIVES: Spontaneous abortion (miscarriage) is a common adverse pregnancy outcome earth-wide, and has remained a challenge in Nigeria. This study aimed at comparing the vaginal microbiome of women who have had episodes of spontaneous abortion with those who have not experienced any incident - in order to find out any possible role of vaginal microbiota in spontaneous abortion. STUDY DESIGN: High vaginal swab samples were collected from the vagina fornix of 6 women of reproductive age, with a history of recurrent spontaneous abortion, as well as those without such history (non-spontaneous abortion). The samples were analyzed and interpreted by standard metagenomic and bioinformatic techniques. RESULTS: The following phyla were encountered in spontaneous abortion and non-spontaneous abortion, respectively: Firmicutes (69.4%, 94.9%), Actinobacteria (12.7%, 1.1%), Bacteroidetes (9.5%, 2.8%), Proteobacteria (7.9%, 0.3%), Chloroflexi (0.2%, 0.0%), Fusobacteria (0.2%, 0.0%), Tenericutes (0.02%, 1.0%). There was more bacterial diversity in spontaneous abortion (H=2.34856) than in Non-spontaneous abortion (H=0.61384), with evenness (EH) of 0.60668 and 0.24703, respectively. On the contrary, Lactobacillus had more relative abundance in non-spontaneous abortion (83%) than spontaneous abortion (23.5%). The following genera (among others) occurred exclusively in spontaneous abortion: Enterococcus (relative abundance=26%), Peptostreptococcus (5.1%), Anaerococcus (2.4%), Dialister (2.1%), Streptococcus (1.9%), Megasphaera (1.3%), Mobiluncus (1.0%), Peptinophilus (0.9%), and Veillonella (0.7%). The efficiency of taxonomic identification, using the operational taxonomic unit clustering method, declined, downstream, from family to species levels. CONCLUSION: Recurrent spontaneous abortion appears to be associated with low vaginal Lactobacillus abundance and high bacterial diversity. We recommend that the current operational taxonomic unit -based sequence taxonomic analysis technique be reviewed. https://gorm.com.tr/index.php/GORM/article/view/1297Eastern nigeriaSpontaneous abortion Vaginal microbiome |
| spellingShingle | Felix Emele Emele Prisca Onyeulor Francisca Nwaokorie Damian Asogwa Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria Gynecology Obstetrics & Reproductive Medicine Eastern nigeria Spontaneous abortion Vaginal microbiome |
| title | Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria |
| title_full | Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria |
| title_fullStr | Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria |
| title_full_unstemmed | Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria |
| title_short | Analysis of Vaginal Microbiome in Women with or Without Episodes of Spontaneous Abortion in Eastern Nigeria |
| title_sort | analysis of vaginal microbiome in women with or without episodes of spontaneous abortion in eastern nigeria |
| topic | Eastern nigeria Spontaneous abortion Vaginal microbiome |
| url | https://gorm.com.tr/index.php/GORM/article/view/1297 |
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