Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province
Introduction. Streptococcus agalactiae are gram-positive, non-motile and encapsulated cocci. On blood agar, they produce an narrow zone of beta-haemolysis. This pathogen causes invasive bacterial diseases in newborns, including sepsis, meningitis, septicaemia, and pneumonia, when transmitted from in...
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Central Research Institute for Epidemiology
2024-12-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/18557/1555 |
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| author | Ali D. Marhash Zainab N. Nabat Nawras A. Abbas |
| author_facet | Ali D. Marhash Zainab N. Nabat Nawras A. Abbas |
| author_sort | Ali D. Marhash |
| collection | DOAJ |
| description | Introduction. Streptococcus agalactiae are gram-positive, non-motile and encapsulated cocci. On blood agar, they produce an narrow zone of beta-haemolysis. This pathogen causes invasive bacterial diseases in newborns, including sepsis, meningitis, septicaemia, and pneumonia, when transmitted from infected mothers. Since S. agalactiae is a pathogen of primary concern for public health, this research has been conducted on it.
The objective of the study is the isolation and molecular detection of virulence gene of S. agalactiae group B (GBS), and evaluation of the percentage of mother-to-child transmission of the pathogen.
Materials and methods. A prospective cohort study was designed that included 300 pregnant women who were at more than 35 weeks of pregnancy. The gynaecologist collected 300 vaginal swabs from all participants in this study and followed up on all GBS-positive pregnant women after delivery to take swabs from their neonates. Traditional microbiological and molecular approaches were used to study isolated bacteria.
Result. Sixty (20%) of three hundred pregnant women and 16 (26.6%) of their newborns were enrolled in this study. GBS was detected via culture methods and was confirmed by PCR with primers employed for the detection of atr gene (housekeeping gene). Positive isolates were 100% susceptible to antibiotics such as ceftriaxone, penicillin, and vancomycin, 93% were sensitive to chloramphenicol, 83% to erythromycin, and only 13% to tetracycline.
Conclusion. Our data showed a high frequency of GBS infection in pregnant women and their newborns. A mandatory screening test and preventative medicine should be adopted to minimize the potentially fatal repercussions of this sickness. |
| format | Article |
| id | doaj-art-e22c17ca79da4e14ac9711a472a45793 |
| institution | Kabale University |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| publishDate | 2024-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj-art-e22c17ca79da4e14ac9711a472a457932025-02-06T21:11:31ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132024-12-01101681281910.36233/0372-9311-5152802Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon provinceAli D. Marhash0https://orcid.org/0000-0003-1078-0541Zainab N. Nabat1https://orcid.org/0000-0001-5517-8146Nawras A. Abbas2https://orcid.org/0000-0002-5818-5525Babylon Technical Institute, Al Furat Al-Awsat Technical universityBabylon Technical Institute, Al Furat Al-Awsat Technical universityBabylon Technical Institute, Al Furat Al-Awsat Technical universityIntroduction. Streptococcus agalactiae are gram-positive, non-motile and encapsulated cocci. On blood agar, they produce an narrow zone of beta-haemolysis. This pathogen causes invasive bacterial diseases in newborns, including sepsis, meningitis, septicaemia, and pneumonia, when transmitted from infected mothers. Since S. agalactiae is a pathogen of primary concern for public health, this research has been conducted on it. The objective of the study is the isolation and molecular detection of virulence gene of S. agalactiae group B (GBS), and evaluation of the percentage of mother-to-child transmission of the pathogen. Materials and methods. A prospective cohort study was designed that included 300 pregnant women who were at more than 35 weeks of pregnancy. The gynaecologist collected 300 vaginal swabs from all participants in this study and followed up on all GBS-positive pregnant women after delivery to take swabs from their neonates. Traditional microbiological and molecular approaches were used to study isolated bacteria. Result. Sixty (20%) of three hundred pregnant women and 16 (26.6%) of their newborns were enrolled in this study. GBS was detected via culture methods and was confirmed by PCR with primers employed for the detection of atr gene (housekeeping gene). Positive isolates were 100% susceptible to antibiotics such as ceftriaxone, penicillin, and vancomycin, 93% were sensitive to chloramphenicol, 83% to erythromycin, and only 13% to tetracycline. Conclusion. Our data showed a high frequency of GBS infection in pregnant women and their newborns. A mandatory screening test and preventative medicine should be adopted to minimize the potentially fatal repercussions of this sickness.https://microbiol.crie.ru/jour/article/viewFile/18557/1555streptococcus agalactiae group bmolecular detectionpregnant women colonizationneonatal colonizationantimicrobial susceptibility |
| spellingShingle | Ali D. Marhash Zainab N. Nabat Nawras A. Abbas Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province Журнал микробиологии, эпидемиологии и иммунобиологии streptococcus agalactiae group b molecular detection pregnant women colonization neonatal colonization antimicrobial susceptibility |
| title | Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province |
| title_full | Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province |
| title_fullStr | Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province |
| title_full_unstemmed | Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province |
| title_short | Molecular detection of <i>Streptococcus agalactiae</i> in pregnant women and percentage of vertical transmission to their neonates in Babylon province |
| title_sort | molecular detection of i streptococcus agalactiae i in pregnant women and percentage of vertical transmission to their neonates in babylon province |
| topic | streptococcus agalactiae group b molecular detection pregnant women colonization neonatal colonization antimicrobial susceptibility |
| url | https://microbiol.crie.ru/jour/article/viewFile/18557/1555 |
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