The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation
OBJECTIVE: Many studies on the interrelation of cumulus cells and oocytes, and research on cumulus protein factors continue. This study aims to investigate the relationship of Pentraxin 3 level with embryo implantation in the cumulus culture fluid. STUDY DESIGN: A total of 31 women with idiopath...
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Medical Network
2022-08-01
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| Series: | Gynecology Obstetrics & Reproductive Medicine |
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| Online Access: | https://gorm.com.tr/index.php/GORM/article/view/1188 |
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| author | Tulay Irez Yavuz Sahin Eduard Malik Onur Guralp |
| author_facet | Tulay Irez Yavuz Sahin Eduard Malik Onur Guralp |
| author_sort | Tulay Irez |
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OBJECTIVE: Many studies on the interrelation of cumulus cells and oocytes, and research on cumulus protein factors continue. This study aims to investigate the relationship of Pentraxin 3 level with embryo implantation in the cumulus culture fluid.
STUDY DESIGN: A total of 31 women with idiopathic infertility who underwent intracytoplasmic sperm injection treatment were prospectively evaluated. Cell suspensions containing 5 million/mL cumulus cells were obtained post-hyase and incubated for 24 hours in a culture medium. A possible association between the culture media Pentraxin 3 concentrations and embryo implantation was analyzed.
RESULTS: The cumulus cell culture media Pentraxin 3 concentrations were significantly higher in the pregnant group compared to the non-pregnant group (98.9 ng/mL vs 53.2 ng/mL, respectively, p=0.005). There was a significant positive correlation between the culture media Pentraxin 3 concentrations and embryo implantation (r=0.500, p=0.005). The culture media Pentraxin 3 concentration was a significant predictor for successful embryo implantation (AUC=0.845, p=0.006). A cut-off value of 64.25 ng/mL had an 86% sensitivity and 80% specificity to predict embryo implantation. There was no pregnancy under the cut-off value of 60 ng/mL, whereby seven women had good quality grade 1 oocytes according to the traditional morphological criteria.
CONCLUSION: The cumulus cell culture medium Pentraxin 3 concentration was predictive for successful embryo implantation. Low Pentraxin 3 levels (<60 ng/mL were associated with failure of conception.
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| format | Article |
| id | doaj-art-e29ca73427d24f198b701036e65f8e29 |
| institution | Kabale University |
| issn | 1300-4751 2602-4918 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Medical Network |
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| series | Gynecology Obstetrics & Reproductive Medicine |
| spelling | doaj-art-e29ca73427d24f198b701036e65f8e292025-02-11T21:15:41ZengMedical NetworkGynecology Obstetrics & Reproductive Medicine1300-47512602-49182022-08-0128210.21613/GORM.2021.1188The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo ImplantationTulay Irez0Yavuz Sahin1Eduard Malik2Onur Guralp3Istanbul Yeni Yuzyil University Faculty of Medicine,Dept.of Histology&EmbryologyMinistry of health, AnkaraDepartment of Gynecology and Obstetrics University of Oldenburg, GermanyDepartment of Gynecology and Obstetrics University of Oldenburg, Germany OBJECTIVE: Many studies on the interrelation of cumulus cells and oocytes, and research on cumulus protein factors continue. This study aims to investigate the relationship of Pentraxin 3 level with embryo implantation in the cumulus culture fluid. STUDY DESIGN: A total of 31 women with idiopathic infertility who underwent intracytoplasmic sperm injection treatment were prospectively evaluated. Cell suspensions containing 5 million/mL cumulus cells were obtained post-hyase and incubated for 24 hours in a culture medium. A possible association between the culture media Pentraxin 3 concentrations and embryo implantation was analyzed. RESULTS: The cumulus cell culture media Pentraxin 3 concentrations were significantly higher in the pregnant group compared to the non-pregnant group (98.9 ng/mL vs 53.2 ng/mL, respectively, p=0.005). There was a significant positive correlation between the culture media Pentraxin 3 concentrations and embryo implantation (r=0.500, p=0.005). The culture media Pentraxin 3 concentration was a significant predictor for successful embryo implantation (AUC=0.845, p=0.006). A cut-off value of 64.25 ng/mL had an 86% sensitivity and 80% specificity to predict embryo implantation. There was no pregnancy under the cut-off value of 60 ng/mL, whereby seven women had good quality grade 1 oocytes according to the traditional morphological criteria. CONCLUSION: The cumulus cell culture medium Pentraxin 3 concentration was predictive for successful embryo implantation. Low Pentraxin 3 levels (<60 ng/mL were associated with failure of conception. https://gorm.com.tr/index.php/GORM/article/view/1188Cumulus granulosa cellPentraxin 3In vitro fertilization |
| spellingShingle | Tulay Irez Yavuz Sahin Eduard Malik Onur Guralp The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation Gynecology Obstetrics & Reproductive Medicine Cumulus granulosa cell Pentraxin 3 In vitro fertilization |
| title | The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation |
| title_full | The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation |
| title_fullStr | The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation |
| title_full_unstemmed | The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation |
| title_short | The Predictive Value of the Pentraxin 3 Concentration in Cumulus Cell Culture Media for the Embryo Implantation |
| title_sort | predictive value of the pentraxin 3 concentration in cumulus cell culture media for the embryo implantation |
| topic | Cumulus granulosa cell Pentraxin 3 In vitro fertilization |
| url | https://gorm.com.tr/index.php/GORM/article/view/1188 |
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