Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients
Abstract Some studies suggest that the respiratory microbiome of COVID-19 patients differs from that of healthy individuals, infected patients may have reduced diversity and increased levels of opportunistic bacteria, however, the role of the microbiome in fatal SARS-CoV-2 infection remains poorly u...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-88518-y |
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| author | L. Johana Madroñero Eliana P. Calvo Carolina Coronel-Ruiz Myriam L. Velandia-Romero María Angélica Calderón-Peláez Jhann A. Arturo Adriana P. Franco-Rodríguez Ricardo Gutiérrez-Pérez Lady S. López Félix G. Delgado Sigrid J. Camacho-Ortega Lilia J. Bernal-Cepeda Sonia P. Bohórquez Jaime E. Castellanos |
| author_facet | L. Johana Madroñero Eliana P. Calvo Carolina Coronel-Ruiz Myriam L. Velandia-Romero María Angélica Calderón-Peláez Jhann A. Arturo Adriana P. Franco-Rodríguez Ricardo Gutiérrez-Pérez Lady S. López Félix G. Delgado Sigrid J. Camacho-Ortega Lilia J. Bernal-Cepeda Sonia P. Bohórquez Jaime E. Castellanos |
| author_sort | L. Johana Madroñero |
| collection | DOAJ |
| description | Abstract Some studies suggest that the respiratory microbiome of COVID-19 patients differs from that of healthy individuals, infected patients may have reduced diversity and increased levels of opportunistic bacteria, however, the role of the microbiome in fatal SARS-CoV-2 infection remains poorly understood. Our study aimed to determine whether there are differences in the respiratory microbiome between patients who recovered from COVID-19 and those who died, by characterizing the bacterial communities of both groups. A total of 24 patients who recovered from COVID-19 and 24 who died were included in the study, patient data were analyzed for signs, symptoms and clinical variables. Airway samples were collected and the 16 S rRNA variable regions V3-V4 were amplified and sequenced using the Illumina MiSeq platform. Elevated levels of blood urea nitrogen, creatinine and lactate dehydrogenase, and higher frequencies of cardiovascular disease, diabetes mellitus and renal disease were observed in patients with a fatal outcome. Compared to patients who recovered from COVID-19, patients who died exhibited a microbiome enriched in periodontal and pathogenic bacteria such as Klebsiella pneumoniae. Our results highlighted a dual relationship between SARS CoV-2 infection and an exacerbated periodontopathogen-induced immune response. |
| format | Article |
| id | doaj-art-e40dc6d27c6344e6bf6d635046d385a6 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e40dc6d27c6344e6bf6d635046d385a62025-02-09T12:32:02ZengNature PortfolioScientific Reports2045-23222025-02-0115111210.1038/s41598-025-88518-yPathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patientsL. Johana Madroñero0Eliana P. Calvo1Carolina Coronel-Ruiz2Myriam L. Velandia-Romero3María Angélica Calderón-Peláez4Jhann A. Arturo5Adriana P. Franco-Rodríguez6Ricardo Gutiérrez-Pérez7Lady S. López8Félix G. Delgado9Sigrid J. Camacho-Ortega10Lilia J. Bernal-Cepeda11Sonia P. Bohórquez12Jaime E. Castellanos13Grupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueClínica MetaClínica MetaGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueGrupo de Virología, Vicerrectoría de Investigaciones, Universidad El BosqueAbstract Some studies suggest that the respiratory microbiome of COVID-19 patients differs from that of healthy individuals, infected patients may have reduced diversity and increased levels of opportunistic bacteria, however, the role of the microbiome in fatal SARS-CoV-2 infection remains poorly understood. Our study aimed to determine whether there are differences in the respiratory microbiome between patients who recovered from COVID-19 and those who died, by characterizing the bacterial communities of both groups. A total of 24 patients who recovered from COVID-19 and 24 who died were included in the study, patient data were analyzed for signs, symptoms and clinical variables. Airway samples were collected and the 16 S rRNA variable regions V3-V4 were amplified and sequenced using the Illumina MiSeq platform. Elevated levels of blood urea nitrogen, creatinine and lactate dehydrogenase, and higher frequencies of cardiovascular disease, diabetes mellitus and renal disease were observed in patients with a fatal outcome. Compared to patients who recovered from COVID-19, patients who died exhibited a microbiome enriched in periodontal and pathogenic bacteria such as Klebsiella pneumoniae. Our results highlighted a dual relationship between SARS CoV-2 infection and an exacerbated periodontopathogen-induced immune response.https://doi.org/10.1038/s41598-025-88518-yMicrobiomeSARS CoV-2COVID-1916S rRNA sequencingFatal outcomeSevere disease |
| spellingShingle | L. Johana Madroñero Eliana P. Calvo Carolina Coronel-Ruiz Myriam L. Velandia-Romero María Angélica Calderón-Peláez Jhann A. Arturo Adriana P. Franco-Rodríguez Ricardo Gutiérrez-Pérez Lady S. López Félix G. Delgado Sigrid J. Camacho-Ortega Lilia J. Bernal-Cepeda Sonia P. Bohórquez Jaime E. Castellanos Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients Scientific Reports Microbiome SARS CoV-2 COVID-19 16S rRNA sequencing Fatal outcome Severe disease |
| title | Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients |
| title_full | Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients |
| title_fullStr | Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients |
| title_full_unstemmed | Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients |
| title_short | Pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly COVID-19 patients |
| title_sort | pathogenic and periodontal bacteria may contribute to the fatal outcome of critically ill elderly covid 19 patients |
| topic | Microbiome SARS CoV-2 COVID-19 16S rRNA sequencing Fatal outcome Severe disease |
| url | https://doi.org/10.1038/s41598-025-88518-y |
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