Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus
Abstract Plant growth behavior is a function of genetic network architecture. The importance of root microbiome variation driving plant functional traits is increasingly recognized, but the genetic mechanisms governing this variation are less studied. Here, we collect roots and rhizosphere soils fro...
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56226-w |
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| author | Jiadong Wu Sijia Liu Haoyu Zhang Sisi Chen Jingna Si Lin Liu Yue Wang Shuxian Tan Yuxin Du Zhelun Jin Jianbo Xie Deqiang Zhang |
| author_facet | Jiadong Wu Sijia Liu Haoyu Zhang Sisi Chen Jingna Si Lin Liu Yue Wang Shuxian Tan Yuxin Du Zhelun Jin Jianbo Xie Deqiang Zhang |
| author_sort | Jiadong Wu |
| collection | DOAJ |
| description | Abstract Plant growth behavior is a function of genetic network architecture. The importance of root microbiome variation driving plant functional traits is increasingly recognized, but the genetic mechanisms governing this variation are less studied. Here, we collect roots and rhizosphere soils from nine Populus species belonging to four sections (Leuce, Aigeiros, Tacamahaca, and Turanga), generate metabolite and transcription data for roots and microbiota data for rhizospheres, and conduct comprehensive multi-omics analyses. We demonstrate that the roots of vigorous Leuce poplar enrich more Pseudomonas, compared with the poorly performing poplar. Moreover, we confirm that Pseudomonas is strongly associated with tricin and apigenin biosynthesis and identify that gene GLABRA3 (GL3) is critical for tricin secretion. The elevated tricin secretion via constitutive transcription of PopGL3 and Chalcone synthase (PopCHS4) can drive Pseudomonas colonization in the rhizosphere and further enhance poplar growth, nitrogen acquisition, and secondary root development in nitrogen-poor soil. This study reveals that plant-metabolite-microbe regulation patterns contribute to the poplar fitness and thoroughly decodes the key regulatory mechanisms of tricin, and provides insights into the interactions of the plant’s key metabolites with its transcriptome and rhizosphere microbes. |
| format | Article |
| id | doaj-art-e5a80c6cd5f54f068c279c196e28fdc6 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e5a80c6cd5f54f068c279c196e28fdc62025-02-09T12:45:44ZengNature PortfolioNature Communications2041-17232025-02-0116111510.1038/s41467-025-56226-wFlavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in PopulusJiadong Wu0Sijia Liu1Haoyu Zhang2Sisi Chen3Jingna Si4Lin Liu5Yue Wang6Shuxian Tan7Yuxin Du8Zhelun Jin9Jianbo Xie10Deqiang Zhang11State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry UniversityAbstract Plant growth behavior is a function of genetic network architecture. The importance of root microbiome variation driving plant functional traits is increasingly recognized, but the genetic mechanisms governing this variation are less studied. Here, we collect roots and rhizosphere soils from nine Populus species belonging to four sections (Leuce, Aigeiros, Tacamahaca, and Turanga), generate metabolite and transcription data for roots and microbiota data for rhizospheres, and conduct comprehensive multi-omics analyses. We demonstrate that the roots of vigorous Leuce poplar enrich more Pseudomonas, compared with the poorly performing poplar. Moreover, we confirm that Pseudomonas is strongly associated with tricin and apigenin biosynthesis and identify that gene GLABRA3 (GL3) is critical for tricin secretion. The elevated tricin secretion via constitutive transcription of PopGL3 and Chalcone synthase (PopCHS4) can drive Pseudomonas colonization in the rhizosphere and further enhance poplar growth, nitrogen acquisition, and secondary root development in nitrogen-poor soil. This study reveals that plant-metabolite-microbe regulation patterns contribute to the poplar fitness and thoroughly decodes the key regulatory mechanisms of tricin, and provides insights into the interactions of the plant’s key metabolites with its transcriptome and rhizosphere microbes.https://doi.org/10.1038/s41467-025-56226-w |
| spellingShingle | Jiadong Wu Sijia Liu Haoyu Zhang Sisi Chen Jingna Si Lin Liu Yue Wang Shuxian Tan Yuxin Du Zhelun Jin Jianbo Xie Deqiang Zhang Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus Nature Communications |
| title | Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus |
| title_full | Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus |
| title_fullStr | Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus |
| title_full_unstemmed | Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus |
| title_short | Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus |
| title_sort | flavones enrich rhizosphere pseudomonas to enhance nitrogen utilization and secondary root growth in populus |
| url | https://doi.org/10.1038/s41467-025-56226-w |
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