Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats
Background: Diabetes mellitus, particularly Type 2 (T2DM), is a global health concern characterized by high blood glucose levels and insulin resistance. Objective: This study aims to explore the hypoglycemic potential of M. oleifera, a plant that thrives in Central Sulawesi, using a rat model of...
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Indonesian Society for Biochemistry and Molecular Biology
2024-08-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/130 |
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| author | Rabiatul Adawiyah Tri Setyawati Rahma Badaruddin Listawati |
| author_facet | Rabiatul Adawiyah Tri Setyawati Rahma Badaruddin Listawati |
| author_sort | Rabiatul Adawiyah |
| collection | DOAJ |
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Background: Diabetes mellitus, particularly Type 2 (T2DM), is a global health concern characterized by high blood glucose levels and insulin resistance.
Objective: This study aims to explore the hypoglycemic potential of M. oleifera, a plant that thrives in Central Sulawesi, using a rat model of T2DM.
Method: Healthy male Wistar rats (weight 200-300 g, aged 9-11 weeks) were used in the experiment. The rats were divided into five groups: normal control (healthy), negative control (diabetic without treatment), positive control (diabetic treated with 9 mg/200 g metformin), treatment 1 (diabetic treated with 400 mg/kg BW M. oleifera extract), and treatment 2 (diabetic treated with 800 mg/kg BW M.oleifera extract). Fasting blood glucose levels were measured enzymatically using the Glucose GOD FS kit from DiaSys. Data were analyzed using ANOVA followed by post-hoc analysis.
Results: The fasting blood glucose levels significantly differed among the groups (ANOVA, p = 0.0426). The normal control group maintained stable glucose levels (108.3 ± 8.34 mg/dL pre-test and 106.6 ± 29.67 mg/dL post-test). The negative control group showed a marked increase in glucose levels (185.3 ± 36.28 mg/dL to 268.6 ± 17.63 mg/dL). The positive control group (metformin) significantly reduced glucose levels (386.8 ± 64.22 mg/dL to 230.8 ± 25.82 mg/dL). Treatment 1 (400 mg/kg BW) reduced glucose levels from 292.2 ± 0.98 mg/dL to 218.1 ± 13.74 mg/dL, and treatment 2 (800 mg/kg BW) showed a substantial reduction from 287.3 ± 85.30 mg/dL to 145.3 ± 28.30 mg/dL. However, the Bonferroni post-hoc analysis indicated no significant differences between pre-test and post-test levels within each group.
Conclusion: The M.oleifera extract exhibited a hypoglycemic effect in diabetic rats, with the higher dose (800 mg/kg BW) showing a more pronounced effect.
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| format | Article |
| id | doaj-art-e67225f5d34e40a8a1bfbee4de1637b8 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-e67225f5d34e40a8a1bfbee4de1637b82025-02-08T03:04:49ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222024-08-017110.32889/actabioina.130Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic ratsRabiatul Adawiyah0Tri Setyawati1Rahma Badaruddin2Listawati3Department of Biochemistry, Faculty of Medicine, Tadulako UniversityDepartment of Biochemistry, Faculty of Medicine, Tadulako UniversityDepartment of Physiology, Faculty of Medicine, Tadulako UniversityDepartment of Biochemistry, Faculty of Medicine, Tadulako University, Palu, 94148, Indonesia Background: Diabetes mellitus, particularly Type 2 (T2DM), is a global health concern characterized by high blood glucose levels and insulin resistance. Objective: This study aims to explore the hypoglycemic potential of M. oleifera, a plant that thrives in Central Sulawesi, using a rat model of T2DM. Method: Healthy male Wistar rats (weight 200-300 g, aged 9-11 weeks) were used in the experiment. The rats were divided into five groups: normal control (healthy), negative control (diabetic without treatment), positive control (diabetic treated with 9 mg/200 g metformin), treatment 1 (diabetic treated with 400 mg/kg BW M. oleifera extract), and treatment 2 (diabetic treated with 800 mg/kg BW M.oleifera extract). Fasting blood glucose levels were measured enzymatically using the Glucose GOD FS kit from DiaSys. Data were analyzed using ANOVA followed by post-hoc analysis. Results: The fasting blood glucose levels significantly differed among the groups (ANOVA, p = 0.0426). The normal control group maintained stable glucose levels (108.3 ± 8.34 mg/dL pre-test and 106.6 ± 29.67 mg/dL post-test). The negative control group showed a marked increase in glucose levels (185.3 ± 36.28 mg/dL to 268.6 ± 17.63 mg/dL). The positive control group (metformin) significantly reduced glucose levels (386.8 ± 64.22 mg/dL to 230.8 ± 25.82 mg/dL). Treatment 1 (400 mg/kg BW) reduced glucose levels from 292.2 ± 0.98 mg/dL to 218.1 ± 13.74 mg/dL, and treatment 2 (800 mg/kg BW) showed a substantial reduction from 287.3 ± 85.30 mg/dL to 145.3 ± 28.30 mg/dL. However, the Bonferroni post-hoc analysis indicated no significant differences between pre-test and post-test levels within each group. Conclusion: The M.oleifera extract exhibited a hypoglycemic effect in diabetic rats, with the higher dose (800 mg/kg BW) showing a more pronounced effect. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/130diabetesMoringa oleiferainsulinhyperglycemiastreptozotocin |
| spellingShingle | Rabiatul Adawiyah Tri Setyawati Rahma Badaruddin Listawati Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats Acta Biochimica Indonesiana diabetes Moringa oleifera insulin hyperglycemia streptozotocin |
| title | Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats |
| title_full | Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats |
| title_fullStr | Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats |
| title_full_unstemmed | Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats |
| title_short | Hypoglychemic activity of Moringa oleifera extract in streptozotocin-induced diabetic rats |
| title_sort | hypoglychemic activity of moringa oleifera extract in streptozotocin induced diabetic rats |
| topic | diabetes Moringa oleifera insulin hyperglycemia streptozotocin |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/130 |
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