GmERF57 negatively regulates root development and phosphate absorption in soybean
Low soil phosphate (Pi) availability is a primary limiting factor for crop growth and production due to its immobilization in soil, thereby impeding Pi uptake by plants. However, excessive supply of phosphorus fertilizer can result in eutrophication of water bodies. Therefore, improving Pi utilizati...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25000284 |
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| author | Hongqing Zhu Dandan Hu Yifei Yang Xuhao Zhai Shanshan Zhang Mengshi He Huifang Zuo Lina Zhang Mengjun Xu Shanshan Chu Haiyan Lü Hengyou Zhang Yu Zhang Dan Zhang |
| author_facet | Hongqing Zhu Dandan Hu Yifei Yang Xuhao Zhai Shanshan Zhang Mengshi He Huifang Zuo Lina Zhang Mengjun Xu Shanshan Chu Haiyan Lü Hengyou Zhang Yu Zhang Dan Zhang |
| author_sort | Hongqing Zhu |
| collection | DOAJ |
| description | Low soil phosphate (Pi) availability is a primary limiting factor for crop growth and production due to its immobilization in soil, thereby impeding Pi uptake by plants. However, excessive supply of phosphorus fertilizer can result in eutrophication of water bodies. Therefore, improving Pi utilization and uptake efficiency in plants is crucial for sustainable agriculture. Previously, we identified soybean Ethylene Response Factor 57 (GmERF57) as a candidate gene responsible for q20, a major QTL associated with soybean low Pi (LP) tolerance-related traits identified through QTL mapping and genome-wide association analysis (GWAS). Population genomics analysis revealed that GmERF57 has undergone artificial selection, and allelic distribution analysis significantly correlated with traits associated with LP tolerance in soybean. Haplotype 2 (Hap2), carrying the T to C single nucleotide polymorphism (SNP), represents the optimal allele favoring LP tolerance. Silencing GmERF57 expression promoted root development and enhanced plant Pi uptake; conversely, overexpression of GmERF57 yielded contrasting phenotypes compared to silenced roots. We further uncovered that GmERF57 physically interacts with GmTUB1 (β-tubulin protein) and modulates soybean root architecture and Pi uptake capacity by downregulating GmTUB1 and other Pi starvation response genes. Overall, the results identified GmERF57 as a QTL gene associated with LP tolerance, elucidated its role in regulating LP tolerance, and further identified an optimal haplotype to facilitate breeding of LP-tolerant soybean cultivars. |
| format | Article |
| id | doaj-art-e896867ae7d346c19766bc255511336d |
| institution | Kabale University |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-e896867ae7d346c19766bc255511336d2025-02-10T04:35:25ZengElsevierPlant Stress2667-064X2025-03-0115100763GmERF57 negatively regulates root development and phosphate absorption in soybeanHongqing Zhu0Dandan Hu1Yifei Yang2Xuhao Zhai3Shanshan Zhang4Mengshi He5Huifang Zuo6Lina Zhang7Mengjun Xu8Shanshan Chu9Haiyan Lü10Hengyou Zhang11Yu Zhang12Dan Zhang13Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Agriculture/Tree Peony, Henan University of Science and Technology, Luoyang 471000, PR ChinaCollege of Information and Management Science, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR ChinaCollege of Information and Management Science, Henan Agricultural University, Zhengzhou 450002, PR ChinaState Key Laboratory of Black Soils Conservation and Utilization, Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150118, PR ChinaCollege of Life Sciences, Henan Agricultural University, Zhengzhou 450002, PR China; Corresponding authors.Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, PR China; Corresponding authors.Low soil phosphate (Pi) availability is a primary limiting factor for crop growth and production due to its immobilization in soil, thereby impeding Pi uptake by plants. However, excessive supply of phosphorus fertilizer can result in eutrophication of water bodies. Therefore, improving Pi utilization and uptake efficiency in plants is crucial for sustainable agriculture. Previously, we identified soybean Ethylene Response Factor 57 (GmERF57) as a candidate gene responsible for q20, a major QTL associated with soybean low Pi (LP) tolerance-related traits identified through QTL mapping and genome-wide association analysis (GWAS). Population genomics analysis revealed that GmERF57 has undergone artificial selection, and allelic distribution analysis significantly correlated with traits associated with LP tolerance in soybean. Haplotype 2 (Hap2), carrying the T to C single nucleotide polymorphism (SNP), represents the optimal allele favoring LP tolerance. Silencing GmERF57 expression promoted root development and enhanced plant Pi uptake; conversely, overexpression of GmERF57 yielded contrasting phenotypes compared to silenced roots. We further uncovered that GmERF57 physically interacts with GmTUB1 (β-tubulin protein) and modulates soybean root architecture and Pi uptake capacity by downregulating GmTUB1 and other Pi starvation response genes. Overall, the results identified GmERF57 as a QTL gene associated with LP tolerance, elucidated its role in regulating LP tolerance, and further identified an optimal haplotype to facilitate breeding of LP-tolerant soybean cultivars.http://www.sciencedirect.com/science/article/pii/S2667064X25000284SoybeanGmerf57Ap2/erf transcription factorLow-phosphate tolerance |
| spellingShingle | Hongqing Zhu Dandan Hu Yifei Yang Xuhao Zhai Shanshan Zhang Mengshi He Huifang Zuo Lina Zhang Mengjun Xu Shanshan Chu Haiyan Lü Hengyou Zhang Yu Zhang Dan Zhang GmERF57 negatively regulates root development and phosphate absorption in soybean Plant Stress Soybean Gmerf57 Ap2/erf transcription factor Low-phosphate tolerance |
| title | GmERF57 negatively regulates root development and phosphate absorption in soybean |
| title_full | GmERF57 negatively regulates root development and phosphate absorption in soybean |
| title_fullStr | GmERF57 negatively regulates root development and phosphate absorption in soybean |
| title_full_unstemmed | GmERF57 negatively regulates root development and phosphate absorption in soybean |
| title_short | GmERF57 negatively regulates root development and phosphate absorption in soybean |
| title_sort | gmerf57 negatively regulates root development and phosphate absorption in soybean |
| topic | Soybean Gmerf57 Ap2/erf transcription factor Low-phosphate tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25000284 |
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