Flow-based In Vivo Method to Enumerate Translating Ribosomes and Translation Elongation Rate
Protein synthesis is by far the most energetically costly cellular process in rapidly dividing cells. Quantifying translating ribosomes in individual cells and their average mRNA transit rate is arduous. Quantitating assembled ribosomes in individual cells requires electron microscopy and does not i...
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Main Authors: | Mina Seedhom, Devin Dersh, Jonathan Yewdell |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2025-01-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=5165&type=0 |
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