Flow-based In Vivo Method to Enumerate Translating Ribosomes and Translation Elongation Rate

Protein synthesis is by far the most energetically costly cellular process in rapidly dividing cells. Quantifying translating ribosomes in individual cells and their average mRNA transit rate is arduous. Quantitating assembled ribosomes in individual cells requires electron microscopy and does not i...

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Bibliographic Details
Main Authors: Mina Seedhom, Devin Dersh, Jonathan Yewdell
Format: Article
Language:English
Published: Bio-protocol LLC 2025-01-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5165&type=0
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