Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering
[Objective] The effect of NtCET4 gene overexpression in regulating tobacco flowering time was analyzed by constructing NtCET4 overexpression plants, and the feasibility of increasing the number of effective leaves of tobacco to improve tobacco yield based on NtCET4 manipulation was discussed. [Me...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Science Press
2025-01-01
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| Series: | Xibei zhiwu xuebao |
| Subjects: | |
| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240426?st=article_issue |
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| Summary: | [Objective] The effect of NtCET4 gene overexpression in regulating tobacco flowering time was
analyzed by constructing NtCET4 overexpression plants, and the feasibility of increasing the number of effective
leaves of tobacco to improve tobacco yield based on NtCET4 manipulation was discussed.
[Methods] Overexpression vector of NtCET4 was constructed. Transgenic individuals were obtained
through the Agrobacterium-mediated leaf disc transformation. The transgenic plants were identified by
PCR and GUS staining. The overexpressed lines were selected from transgenic lines based on qRT-PCR.
The overexpressed plants were transplanted into fields to observe flowering time and leaf numbers. qRTPCR
was used to identify relative expressions of NtCET4 before and after flowering. [Results] During the
seedling stage, four transgenic lines showed significant increase in NtCET4 expression. These four transgenic
lines with NtCET4 overexpression showed delaying flowering (about 14 days). Longer period of
vegetative bud activity resulted in more valid leaves in the transgenic plants. NtCET4 expression in transgenic
plants was increased from seedling to mature, and higher expression was detected before flowering
relative to after flowering. [Conclusion] Overexpression of NtCET4 promotes the vegetative bud activity,
delays flowering, and increases the number of valid leaves in tobacco. |
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| ISSN: | 1000-4025 |