Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering
[Objective] The effect of NtCET4 gene overexpression in regulating tobacco flowering time was analyzed by constructing NtCET4 overexpression plants, and the feasibility of increasing the number of effective leaves of tobacco to improve tobacco yield based on NtCET4 manipulation was discussed. [Me...
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| Format: | Article |
| Language: | zho |
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Science Press
2025-01-01
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| Series: | Xibei zhiwu xuebao |
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| Online Access: | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240426?st=article_issue |
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| _version_ | 1823858159052128256 |
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| author | LONG Junlan LUO Rui LUO Xu ZHANG Lan |
| author_facet | LONG Junlan LUO Rui LUO Xu ZHANG Lan |
| author_sort | LONG Junlan |
| collection | DOAJ |
| description | [Objective] The effect of NtCET4 gene overexpression in regulating tobacco flowering time was
analyzed by constructing NtCET4 overexpression plants, and the feasibility of increasing the number of effective
leaves of tobacco to improve tobacco yield based on NtCET4 manipulation was discussed.
[Methods] Overexpression vector of NtCET4 was constructed. Transgenic individuals were obtained
through the Agrobacterium-mediated leaf disc transformation. The transgenic plants were identified by
PCR and GUS staining. The overexpressed lines were selected from transgenic lines based on qRT-PCR.
The overexpressed plants were transplanted into fields to observe flowering time and leaf numbers. qRTPCR
was used to identify relative expressions of NtCET4 before and after flowering. [Results] During the
seedling stage, four transgenic lines showed significant increase in NtCET4 expression. These four transgenic
lines with NtCET4 overexpression showed delaying flowering (about 14 days). Longer period of
vegetative bud activity resulted in more valid leaves in the transgenic plants. NtCET4 expression in transgenic
plants was increased from seedling to mature, and higher expression was detected before flowering
relative to after flowering. [Conclusion] Overexpression of NtCET4 promotes the vegetative bud activity,
delays flowering, and increases the number of valid leaves in tobacco. |
| format | Article |
| id | doaj-art-e9dfc65021ca4f65bb7a94f74788f474 |
| institution | Kabale University |
| issn | 1000-4025 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Science Press |
| record_format | Article |
| series | Xibei zhiwu xuebao |
| spelling | doaj-art-e9dfc65021ca4f65bb7a94f74788f4742025-02-11T13:47:12ZzhoScience PressXibei zhiwu xuebao1000-40252025-01-014510011001810.7606/j.issn.1000-4025.20240426Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed floweringLONG Junlan0LUO Rui1LUO Xu2ZHANG Lan3College of Life Sciences, Guizhou University, Guiyang 550025, China; Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region [Ministry of Education], Guizhou University, Guiyang 550025, ChinaCollege of Life Sciences, Guizhou University, Guiyang 550025, ChinaCollege of Life Sciences, Guizhou University, Guiyang 550025, China; Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region [Ministry of Education], Guizhou University, Guiyang 550025, ChinaCollege of Life Sciences, Guizhou University, Guiyang 550025, China; Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region [Ministry of Education], Guizhou University, Guiyang 550025, China[Objective] The effect of NtCET4 gene overexpression in regulating tobacco flowering time was analyzed by constructing NtCET4 overexpression plants, and the feasibility of increasing the number of effective leaves of tobacco to improve tobacco yield based on NtCET4 manipulation was discussed. [Methods] Overexpression vector of NtCET4 was constructed. Transgenic individuals were obtained through the Agrobacterium-mediated leaf disc transformation. The transgenic plants were identified by PCR and GUS staining. The overexpressed lines were selected from transgenic lines based on qRT-PCR. The overexpressed plants were transplanted into fields to observe flowering time and leaf numbers. qRTPCR was used to identify relative expressions of NtCET4 before and after flowering. [Results] During the seedling stage, four transgenic lines showed significant increase in NtCET4 expression. These four transgenic lines with NtCET4 overexpression showed delaying flowering (about 14 days). Longer period of vegetative bud activity resulted in more valid leaves in the transgenic plants. NtCET4 expression in transgenic plants was increased from seedling to mature, and higher expression was detected before flowering relative to after flowering. [Conclusion] Overexpression of NtCET4 promotes the vegetative bud activity, delays flowering, and increases the number of valid leaves in tobacco.http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240426?st=article_issuetobaccontcet4 geneflowering timenumber of valid leaves |
| spellingShingle | LONG Junlan LUO Rui LUO Xu ZHANG Lan Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering Xibei zhiwu xuebao tobacco ntcet4 gene flowering time number of valid leaves |
| title | Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering |
| title_full | Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering |
| title_fullStr | Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering |
| title_full_unstemmed | Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering |
| title_short | Overexpression of NtCET4 gene in tobacco promoted vegetative bud activity and delayed flowering |
| title_sort | overexpression of ntcet4 gene in tobacco promoted vegetative bud activity and delayed flowering |
| topic | tobacco ntcet4 gene flowering time number of valid leaves |
| url | http://xbzwxb.alljournal.net/xbzwxb/article/abstract/20240426?st=article_issue |
| work_keys_str_mv | AT longjunlan overexpressionofntcet4geneintobaccopromotedvegetativebudactivityanddelayedflowering AT luorui overexpressionofntcet4geneintobaccopromotedvegetativebudactivityanddelayedflowering AT luoxu overexpressionofntcet4geneintobaccopromotedvegetativebudactivityanddelayedflowering AT zhanglan overexpressionofntcet4geneintobaccopromotedvegetativebudactivityanddelayedflowering |