24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism
Oxytetracycline (OTC), a crop-absorbable antibiotic, poses a health risk to humans through the food chain. Conversely, 24-epibrassinolide (EBL), a plant growth hormone, mitigates the toxic effects of various pollutants on plants. However, the mechanism by which exogenous EBL affects the growth of ra...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000995 |
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| author | Suya Chen Shanshan Zhou Xuan Xuan Lili Niu Lu Zhao Jingjie Guo Dongmei Xu |
| author_facet | Suya Chen Shanshan Zhou Xuan Xuan Lili Niu Lu Zhao Jingjie Guo Dongmei Xu |
| author_sort | Suya Chen |
| collection | DOAJ |
| description | Oxytetracycline (OTC), a crop-absorbable antibiotic, poses a health risk to humans through the food chain. Conversely, 24-epibrassinolide (EBL), a plant growth hormone, mitigates the toxic effects of various pollutants on plants. However, the mechanism by which exogenous EBL affects the growth of rape seedlings exposed to OTC remains largely unknown. In this study, we found that environmental OTC concentrations significantly inhibited plant growth and metabolism, whereas exogenous EBL could restore plant growth characteristics. Exogenous EBL significantly decreased reactive oxygen species (ROS) accumulation, alleviating OTC-induced cell membrane lipid peroxidation. This was achieved by increasing the antioxidant capacity and secondary metabolism levels. Notably, our findings suggested that EBL stimulated glutathione S-transferase (GST) and glutathione reductase (GR) activities, enhancing reduced glutathione synthesis and participating in plant OTC detoxification. OTC residues in EBL + OTC-treated seedlings at 21 d were significantly reduced by 29 % compared with OTC alone. Further transcriptomic and metabolomic analyses revealed that the differentially expressed genes and metabolites in the EBL and OTC alone or combined treatment groups were primarily involved in the regulation of phenylpropanoid biosynthesis, glutathione metabolism, and lant hormone signal transduction pathways in response to phytotoxic effects and detoxification mechanisms, as compared to the control group. |
| format | Article |
| id | doaj-art-ec02826c1aba46da9f5c448249e5ea18 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-ec02826c1aba46da9f5c448249e5ea182025-02-12T05:30:15ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-0129011776324-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanismSuya Chen0Shanshan Zhou1Xuan Xuan2Lili Niu3Lu Zhao4Jingjie Guo5Dongmei Xu6College of Environment, Zhejiang University of Technology, Hangzhou 310032, China; Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou 310032, ChinaCollege of Environment, Zhejiang University of Technology, Hangzhou 310032, China; Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaKey Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy (IRA), Zhejiang Shuren University, Hangzhou 310015, ChinaKey Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy (IRA), Zhejiang Shuren University, Hangzhou 310015, ChinaKey Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, ChinaKey Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China; Corresponding author.Oxytetracycline (OTC), a crop-absorbable antibiotic, poses a health risk to humans through the food chain. Conversely, 24-epibrassinolide (EBL), a plant growth hormone, mitigates the toxic effects of various pollutants on plants. However, the mechanism by which exogenous EBL affects the growth of rape seedlings exposed to OTC remains largely unknown. In this study, we found that environmental OTC concentrations significantly inhibited plant growth and metabolism, whereas exogenous EBL could restore plant growth characteristics. Exogenous EBL significantly decreased reactive oxygen species (ROS) accumulation, alleviating OTC-induced cell membrane lipid peroxidation. This was achieved by increasing the antioxidant capacity and secondary metabolism levels. Notably, our findings suggested that EBL stimulated glutathione S-transferase (GST) and glutathione reductase (GR) activities, enhancing reduced glutathione synthesis and participating in plant OTC detoxification. OTC residues in EBL + OTC-treated seedlings at 21 d were significantly reduced by 29 % compared with OTC alone. Further transcriptomic and metabolomic analyses revealed that the differentially expressed genes and metabolites in the EBL and OTC alone or combined treatment groups were primarily involved in the regulation of phenylpropanoid biosynthesis, glutathione metabolism, and lant hormone signal transduction pathways in response to phytotoxic effects and detoxification mechanisms, as compared to the control group.http://www.sciencedirect.com/science/article/pii/S0147651325000995Oxytetracycline24-epibrassinolideOxidative stressSecondary metabolismDetoxificationTranscriptome and metabolome |
| spellingShingle | Suya Chen Shanshan Zhou Xuan Xuan Lili Niu Lu Zhao Jingjie Guo Dongmei Xu 24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism Ecotoxicology and Environmental Safety Oxytetracycline 24-epibrassinolide Oxidative stress Secondary metabolism Detoxification Transcriptome and metabolome |
| title | 24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism |
| title_full | 24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism |
| title_fullStr | 24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism |
| title_full_unstemmed | 24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism |
| title_short | 24-epibrassinolide regulates oxytetracycline-induced phytotoxicity and its detoxification mechanism |
| title_sort | 24 epibrassinolide regulates oxytetracycline induced phytotoxicity and its detoxification mechanism |
| topic | Oxytetracycline 24-epibrassinolide Oxidative stress Secondary metabolism Detoxification Transcriptome and metabolome |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000995 |
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