Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae
Saccharomyces cerevisiae (S. cerevisiae) is a microorganism of high interest due to its applications in pharmaceutical and food industries. However, traditional downstream processing of intracellular compounds often depends on organic solvents and harsh processing conditions. Here, the use of hydrop...
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Elsevier
2025-03-01
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| Series: | Results in Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590123025003184 |
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| author | Tjalling Gijsbert Tjalsma Yannick Patrice Didion Ziran Su Magdalena Malankowska Pablo Torres-Montero José Luis Martínez Manuel Pinelo |
| author_facet | Tjalling Gijsbert Tjalsma Yannick Patrice Didion Ziran Su Magdalena Malankowska Pablo Torres-Montero José Luis Martínez Manuel Pinelo |
| author_sort | Tjalling Gijsbert Tjalsma |
| collection | DOAJ |
| description | Saccharomyces cerevisiae (S. cerevisiae) is a microorganism of high interest due to its applications in pharmaceutical and food industries. However, traditional downstream processing of intracellular compounds often depends on organic solvents and harsh processing conditions. Here, the use of hydrophobic deep eutectic solvents (DESs) as cell wall permeabilization agents for intracellular protein release from Saccharomyces cerevisiae was studied for the first time. This study examines the relatively new type V DESs, which favors downstream methods because less solvent is needed and environmentally friendly substances can be used. The DESs were synthesized from L-menthol, lidocaine, acetic acid, decanoic acid, oleic acid and lauric acid, and screened for viability. Next, 0.5 wt% DES was mixed in a mild alkaline buffer containing S. cerevisiae. Despite the similar structures of the hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), DESs with L-menthol and lidocaine as HBA and a fatty acid as HBD exhibited superior performance compared to DESs consisting solely of L-menthol and lidocaine or those derived from fatty acids only. DES lidocaine:decanoic acid 1:3 resulted in a protein yield of 2.45 (±0.03) mg/mL, which outperformed a method using a standard lysis reagent that resulted in 2.33 (±0.04) mg/mL. It was concluded that the underlying linkages between HBA and HBD play a key role because different HBA and HBD combinations have a bearing on whether protein release is successful. |
| format | Article |
| id | doaj-art-f04bb6a9676042e4af8aef47001c9043 |
| institution | Kabale University |
| issn | 2590-1230 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Engineering |
| spelling | doaj-art-f04bb6a9676042e4af8aef47001c90432025-02-08T05:00:59ZengElsevierResults in Engineering2590-12302025-03-0125104232Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiaeTjalling Gijsbert Tjalsma0Yannick Patrice Didion1Ziran Su2Magdalena Malankowska3Pablo Torres-Montero4José Luis Martínez5Manuel Pinelo6Process and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, DenmarkProcess and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, DenmarkProcess and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, DenmarkProcess and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, DenmarkSection for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs. Lyngby, DenmarkSection for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kgs. Lyngby, DenmarkProcess and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark; Corresponding author.Saccharomyces cerevisiae (S. cerevisiae) is a microorganism of high interest due to its applications in pharmaceutical and food industries. However, traditional downstream processing of intracellular compounds often depends on organic solvents and harsh processing conditions. Here, the use of hydrophobic deep eutectic solvents (DESs) as cell wall permeabilization agents for intracellular protein release from Saccharomyces cerevisiae was studied for the first time. This study examines the relatively new type V DESs, which favors downstream methods because less solvent is needed and environmentally friendly substances can be used. The DESs were synthesized from L-menthol, lidocaine, acetic acid, decanoic acid, oleic acid and lauric acid, and screened for viability. Next, 0.5 wt% DES was mixed in a mild alkaline buffer containing S. cerevisiae. Despite the similar structures of the hydrogen bond acceptor (HBA) and hydrogen bond donor (HBD), DESs with L-menthol and lidocaine as HBA and a fatty acid as HBD exhibited superior performance compared to DESs consisting solely of L-menthol and lidocaine or those derived from fatty acids only. DES lidocaine:decanoic acid 1:3 resulted in a protein yield of 2.45 (±0.03) mg/mL, which outperformed a method using a standard lysis reagent that resulted in 2.33 (±0.04) mg/mL. It was concluded that the underlying linkages between HBA and HBD play a key role because different HBA and HBD combinations have a bearing on whether protein release is successful.http://www.sciencedirect.com/science/article/pii/S2590123025003184Hydrophobic deep eutectic solventsIntracellular proteinsSaccharomyces cerevisiaeGreen chemistry |
| spellingShingle | Tjalling Gijsbert Tjalsma Yannick Patrice Didion Ziran Su Magdalena Malankowska Pablo Torres-Montero José Luis Martínez Manuel Pinelo Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae Results in Engineering Hydrophobic deep eutectic solvents Intracellular proteins Saccharomyces cerevisiae Green chemistry |
| title | Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae |
| title_full | Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae |
| title_fullStr | Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae |
| title_full_unstemmed | Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae |
| title_short | Hydrophobic deep eutectic solvents as novel, sustainable aids for intracellular protein release from Saccharomyces cerevisiae |
| title_sort | hydrophobic deep eutectic solvents as novel sustainable aids for intracellular protein release from saccharomyces cerevisiae |
| topic | Hydrophobic deep eutectic solvents Intracellular proteins Saccharomyces cerevisiae Green chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2590123025003184 |
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