CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice
The tolerance of rice to drought and saline stress is crucial for maintaining yields and promoting widespread cultivation. From an ethyl methanesulfonate (EMS)-mutagenized mutant library, we identified a mutant that is susceptible to osmotic stress, named Osmotic Stress Sensitivity 1 (Oss1). Using M...
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Elsevier
2025-01-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S167263082400088X |
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| author | Yu Shicong Luo Ruxian Zheng Shuqin Ning Jing Shi Yuanzhu Guo Daiming Jia Liangmeng Wang Sen Xiao Guizong Guo Pengwang Li Yang Ma Xiaoding |
| author_facet | Yu Shicong Luo Ruxian Zheng Shuqin Ning Jing Shi Yuanzhu Guo Daiming Jia Liangmeng Wang Sen Xiao Guizong Guo Pengwang Li Yang Ma Xiaoding |
| author_sort | Yu Shicong |
| collection | DOAJ |
| description | The tolerance of rice to drought and saline stress is crucial for maintaining yields and promoting widespread cultivation. From an ethyl methanesulfonate (EMS)-mutagenized mutant library, we identified a mutant that is susceptible to osmotic stress, named Osmotic Stress Sensitivity 1 (Oss1). Using MutMap sequencing, we characterized the role of a choline transporter-related family gene, CTR4 (Choline Transporter-Related 4), in rice’s tolerance to drought and salt stress. CTR4 plays a critical role in regulating membrane lipid synthesis. In knockout mutants, the total membrane lipid content, especially unsaturated fatty acids, was significantly reduced. Compared with the wild type, knockout mutants exhibited decreased membrane lipid stability under drought and salt stress, faster water loss, higher relative electrolyte leakage, and lower levels of proline and soluble sugars, leading to impaired tolerance to drought and salt stress. In contrast, the overexpression of CTR4 enhanced seedling tolerance to drought and saline stress. The overexpression lines displayed lower malondialdehyde levels, reduced relative electrolyte leakage, and slower rates of leaf water loss under stress conditions, thereby improving seedling survival rates during stress. Moreover, lipid synthesis gene expression was down-regulated in CTR4 mutants, potentially exacerbating membrane permeability defects and further compromising stress resistance. These findings suggest that CTR4 mediates choline transport and influences cell membrane formation, thereby enhancing rice defenses against drought and salt stress by maintaining lipid homeostasis. |
| format | Article |
| id | doaj-art-f245d01e751c4302ad858ba0cc534fb4 |
| institution | Kabale University |
| issn | 1672-6308 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj-art-f245d01e751c4302ad858ba0cc534fb42025-02-12T05:30:48ZengElsevierRice Science1672-63082025-01-013215266CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in RiceYu Shicong0Luo Ruxian1Zheng Shuqin2Ning Jing3Shi Yuanzhu4Guo Daiming5Jia Liangmeng6Wang Sen7Xiao Guizong8Guo Pengwang9Li Yang10Ma Xiaoding11State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611134, ChinaNational Crop Genebank, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding author.State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding author.The tolerance of rice to drought and saline stress is crucial for maintaining yields and promoting widespread cultivation. From an ethyl methanesulfonate (EMS)-mutagenized mutant library, we identified a mutant that is susceptible to osmotic stress, named Osmotic Stress Sensitivity 1 (Oss1). Using MutMap sequencing, we characterized the role of a choline transporter-related family gene, CTR4 (Choline Transporter-Related 4), in rice’s tolerance to drought and salt stress. CTR4 plays a critical role in regulating membrane lipid synthesis. In knockout mutants, the total membrane lipid content, especially unsaturated fatty acids, was significantly reduced. Compared with the wild type, knockout mutants exhibited decreased membrane lipid stability under drought and salt stress, faster water loss, higher relative electrolyte leakage, and lower levels of proline and soluble sugars, leading to impaired tolerance to drought and salt stress. In contrast, the overexpression of CTR4 enhanced seedling tolerance to drought and saline stress. The overexpression lines displayed lower malondialdehyde levels, reduced relative electrolyte leakage, and slower rates of leaf water loss under stress conditions, thereby improving seedling survival rates during stress. Moreover, lipid synthesis gene expression was down-regulated in CTR4 mutants, potentially exacerbating membrane permeability defects and further compromising stress resistance. These findings suggest that CTR4 mediates choline transport and influences cell membrane formation, thereby enhancing rice defenses against drought and salt stress by maintaining lipid homeostasis.http://www.sciencedirect.com/science/article/pii/S167263082400088XOryza sativadrought stresssalt stresscholine transporterlipid homeostasis |
| spellingShingle | Yu Shicong Luo Ruxian Zheng Shuqin Ning Jing Shi Yuanzhu Guo Daiming Jia Liangmeng Wang Sen Xiao Guizong Guo Pengwang Li Yang Ma Xiaoding CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice Rice Science Oryza sativa drought stress salt stress choline transporter lipid homeostasis |
| title | CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice |
| title_full | CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice |
| title_fullStr | CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice |
| title_full_unstemmed | CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice |
| title_short | CHOLINE TRANSPORTER-RELATED 4 (CTR4) Is Involved in Drought and Saline Tolerance in Rice |
| title_sort | choline transporter related 4 ctr4 is involved in drought and saline tolerance in rice |
| topic | Oryza sativa drought stress salt stress choline transporter lipid homeostasis |
| url | http://www.sciencedirect.com/science/article/pii/S167263082400088X |
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