CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects
Abstract Cotton (Gossypium hirsutum L.) is one of the most important global crops that supports the textile industry and provides a living for millions of farmers. The constantly increasing demand needs a significant rise in cotton production. Genome editing technology, specifically with clustered r...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-02-01
|
| Series: | Journal of Cotton Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42397-024-00206-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823863447184474112 |
|---|---|
| author | Vijay Sheri Harikrishnan Mohan Phanikanth Jogam Anshu Alok Gulab Khan Rohela Baohong Zhang |
| author_facet | Vijay Sheri Harikrishnan Mohan Phanikanth Jogam Anshu Alok Gulab Khan Rohela Baohong Zhang |
| author_sort | Vijay Sheri |
| collection | DOAJ |
| description | Abstract Cotton (Gossypium hirsutum L.) is one of the most important global crops that supports the textile industry and provides a living for millions of farmers. The constantly increasing demand needs a significant rise in cotton production. Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities for trait development in cotton. It allows precise and efficient manipulation within the cotton genome when compared with other genetic engineering tools. Current developments in CRISPR/Cas technology, including prime editing, base editing, and multiplexing editing, have expanded the scope of traits in cotton breeding that can be targeted. CRISPR/Cas genome editing has been employed to generate effectively CRISPRized cotton plants with enhanced agronomic traits, including fiber yield and quality, oil improvement, stress resistance, and enhanced nutrition. Here we summarized the various target genes within the cotton genome which have been successfully altered with CRISPR/Cas tools. However, some challenges remain, cotton is tetraploid genome having redundant gene sets and homologs making challenges for genome editing. To ensure specificity and avoiding off-target effects, we need to optimize various parameters such as target site, guide RNA design, and choosing right Cas variants. We outline the future prospects of CRISPR/Cas in cotton breeding, suggesting areas for further research and innovation. A combination of speed breeding and CRISPR/Cas might be useful for fastening trait development in cotton. The potentials to create customized cotton cultivars with enhanced traits to meet the higher demands for the agriculture and textile industry. |
| format | Article |
| id | doaj-art-f40fc05179e44f508816cc79dfa29178 |
| institution | Kabale University |
| issn | 2523-3254 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cotton Research |
| spelling | doaj-art-f40fc05179e44f508816cc79dfa291782025-02-09T12:09:41ZengBMCJournal of Cotton Research2523-32542025-02-018111410.1186/s42397-024-00206-wCRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospectsVijay Sheri0Harikrishnan Mohan1Phanikanth Jogam2Anshu Alok3Gulab Khan Rohela4Baohong Zhang5Department of Biology, East Carolina UniversityDepartment of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State UniversityDepartment of Biotechnology, Kakatiya UniversityDepartment of Plant Pathology, University of MinnesotaCentral Sericultural Research & Training Institute, Central Silk BoardDepartment of Biology, East Carolina UniversityAbstract Cotton (Gossypium hirsutum L.) is one of the most important global crops that supports the textile industry and provides a living for millions of farmers. The constantly increasing demand needs a significant rise in cotton production. Genome editing technology, specifically with clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) tools, has opened new possibilities for trait development in cotton. It allows precise and efficient manipulation within the cotton genome when compared with other genetic engineering tools. Current developments in CRISPR/Cas technology, including prime editing, base editing, and multiplexing editing, have expanded the scope of traits in cotton breeding that can be targeted. CRISPR/Cas genome editing has been employed to generate effectively CRISPRized cotton plants with enhanced agronomic traits, including fiber yield and quality, oil improvement, stress resistance, and enhanced nutrition. Here we summarized the various target genes within the cotton genome which have been successfully altered with CRISPR/Cas tools. However, some challenges remain, cotton is tetraploid genome having redundant gene sets and homologs making challenges for genome editing. To ensure specificity and avoiding off-target effects, we need to optimize various parameters such as target site, guide RNA design, and choosing right Cas variants. We outline the future prospects of CRISPR/Cas in cotton breeding, suggesting areas for further research and innovation. A combination of speed breeding and CRISPR/Cas might be useful for fastening trait development in cotton. The potentials to create customized cotton cultivars with enhanced traits to meet the higher demands for the agriculture and textile industry.https://doi.org/10.1186/s42397-024-00206-wCRISPR/CasBiotic stressFiber qualityGenomic complexityOff-target effectsTextile industry |
| spellingShingle | Vijay Sheri Harikrishnan Mohan Phanikanth Jogam Anshu Alok Gulab Khan Rohela Baohong Zhang CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects Journal of Cotton Research CRISPR/Cas Biotic stress Fiber quality Genomic complexity Off-target effects Textile industry |
| title | CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects |
| title_full | CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects |
| title_fullStr | CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects |
| title_full_unstemmed | CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects |
| title_short | CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects |
| title_sort | crispr cas genome editing for cotton precision breeding mechanisms advances and prospects |
| topic | CRISPR/Cas Biotic stress Fiber quality Genomic complexity Off-target effects Textile industry |
| url | https://doi.org/10.1186/s42397-024-00206-w |
| work_keys_str_mv | AT vijaysheri crisprcasgenomeeditingforcottonprecisionbreedingmechanismsadvancesandprospects AT harikrishnanmohan crisprcasgenomeeditingforcottonprecisionbreedingmechanismsadvancesandprospects AT phanikanthjogam crisprcasgenomeeditingforcottonprecisionbreedingmechanismsadvancesandprospects AT anshualok crisprcasgenomeeditingforcottonprecisionbreedingmechanismsadvancesandprospects AT gulabkhanrohela crisprcasgenomeeditingforcottonprecisionbreedingmechanismsadvancesandprospects AT baohongzhang crisprcasgenomeeditingforcottonprecisionbreedingmechanismsadvancesandprospects |