Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities
Amomum tsaoko is an important homologous medicinal and food plant, and its fruit is rich in flavonoids. However, few studies have reported the preparation and bioactivity of flavonoids in A. tsaoko (ATF). In this study, the optimal conditions for ultrasound-assisted extraction of ATF were identified...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000239 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856709244813312 |
|---|---|
| author | Zelin Huang Yan Zhao Weixing Yang Lu Lang Jun Sheng Yang Tian Xiaoyu Gao |
| author_facet | Zelin Huang Yan Zhao Weixing Yang Lu Lang Jun Sheng Yang Tian Xiaoyu Gao |
| author_sort | Zelin Huang |
| collection | DOAJ |
| description | Amomum tsaoko is an important homologous medicinal and food plant, and its fruit is rich in flavonoids. However, few studies have reported the preparation and bioactivity of flavonoids in A. tsaoko (ATF). In this study, the optimal conditions for ultrasound-assisted extraction of ATF were identified through response surface optimization. HPD300 was identified as the best resin for the purification of ATF, as it exhibited a Freundlich model-conformative adsorption isotherm. Among the different concentrations of ethanol, 20 % and 30 % resulted in higher flavonoid purity (>90 %) and stronger antioxidant and α-glucosidase inhibition activities. A widely targeted metabolomics assay revealed that the relative abundance of flavonoids in a mixture of 20 % and 30 % ethanol eluates was greater than 73 %, which mainly contained (+)-epicatechin, isoquercitrin, astragalin kaempferol-3-O-rutinoside, and procyanidin B2. These findings provide a theoretical basis for the in-depth development and potential use of ATF in the functional food, cosmetic and pharmaceutical industries. |
| format | Article |
| id | doaj-art-f477981b856a4c53baeec66137d22da3 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-f477981b856a4c53baeec66137d22da32025-02-12T05:32:24ZengElsevierFood Chemistry: X2590-15752025-01-0125102177Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activitiesZelin Huang0Yan Zhao1Weixing Yang2Lu Lang3Jun Sheng4Yang Tian5Xiaoyu Gao6Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, ChinaDivision of Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, ChinaYunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China; Corresponding author at: Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China.Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming 650201, China; College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming 650201, China; Corresponding author.Amomum tsaoko is an important homologous medicinal and food plant, and its fruit is rich in flavonoids. However, few studies have reported the preparation and bioactivity of flavonoids in A. tsaoko (ATF). In this study, the optimal conditions for ultrasound-assisted extraction of ATF were identified through response surface optimization. HPD300 was identified as the best resin for the purification of ATF, as it exhibited a Freundlich model-conformative adsorption isotherm. Among the different concentrations of ethanol, 20 % and 30 % resulted in higher flavonoid purity (>90 %) and stronger antioxidant and α-glucosidase inhibition activities. A widely targeted metabolomics assay revealed that the relative abundance of flavonoids in a mixture of 20 % and 30 % ethanol eluates was greater than 73 %, which mainly contained (+)-epicatechin, isoquercitrin, astragalin kaempferol-3-O-rutinoside, and procyanidin B2. These findings provide a theoretical basis for the in-depth development and potential use of ATF in the functional food, cosmetic and pharmaceutical industries.http://www.sciencedirect.com/science/article/pii/S2590157525000239FlavonoidsUltrasound-assisted extractionMacroporous resinAmomum tsaokoWidely targeted metabolomics |
| spellingShingle | Zelin Huang Yan Zhao Weixing Yang Lu Lang Jun Sheng Yang Tian Xiaoyu Gao Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities Food Chemistry: X Flavonoids Ultrasound-assisted extraction Macroporous resin Amomum tsaoko Widely targeted metabolomics |
| title | Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities |
| title_full | Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities |
| title_fullStr | Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities |
| title_full_unstemmed | Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities |
| title_short | Preparation of flavonoids from Amomum tsaoko and evaluation of their antioxidant and α-glucosidase inhibitory activities |
| title_sort | preparation of flavonoids from amomum tsaoko and evaluation of their antioxidant and α glucosidase inhibitory activities |
| topic | Flavonoids Ultrasound-assisted extraction Macroporous resin Amomum tsaoko Widely targeted metabolomics |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000239 |
| work_keys_str_mv | AT zelinhuang preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities AT yanzhao preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities AT weixingyang preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities AT lulang preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities AT junsheng preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities AT yangtian preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities AT xiaoyugao preparationofflavonoidsfromamomumtsaokoandevaluationoftheirantioxidantandaglucosidaseinhibitoryactivities |