The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions
Extracellular vesicles (EVs) are cell-derived, membrane-bound vesicles that carry molecular cargo to facilitate communication between cells. During pregnancy, EVs are secreted by the syncytiotrophoblast layer of the placenta villi, where they mediate the functions of resident leukocytes and invading...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1528714/full |
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| author | Kunfeng Bai Kunfeng Bai Xintong Li Yanjie Guo Ye Shang Leqian Lin Philip C. N. Chiu Philip C. N. Chiu Cheuk-Lun Lee |
| author_facet | Kunfeng Bai Kunfeng Bai Xintong Li Yanjie Guo Ye Shang Leqian Lin Philip C. N. Chiu Philip C. N. Chiu Cheuk-Lun Lee |
| author_sort | Kunfeng Bai |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are cell-derived, membrane-bound vesicles that carry molecular cargo to facilitate communication between cells. During pregnancy, EVs are secreted by the syncytiotrophoblast layer of the placenta villi, where they mediate the functions of resident leukocytes and invading extravillous trophoblasts (EVTs) in the decidua. This study aims to isolate placental EVs (pEVs) from placental explant to examine their regulatory roles on EVT and endothelial cell functions. pEVs were successfully isolated from ex vivo cultured placental explant, which were capable to be internalized by EVTs and endothelial cells. pEVs stimulated the differentiation of trophoblast stem cells (TSCs) and enhanced the migration and invasion abilities of EVTs via CD147 receptor. Conversely, pEVs inhibited the tube formation ability and interleukin-6 (IL-6) secretion of endothelial cells. Together, these findings partially elucidate the role of pEVs during early pregnancy establishment, which may provide insights into pregnancy-related disorders. |
| format | Article |
| id | doaj-art-f54d20bf60d945c0bc7d6f6b137a3997 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-f54d20bf60d945c0bc7d6f6b137a39972025-02-10T06:48:40ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-02-011310.3389/fcell.2025.15287141528714The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functionsKunfeng Bai0Kunfeng Bai1Xintong Li2Yanjie Guo3Ye Shang4Leqian Lin5Philip C. N. Chiu6Philip C. N. Chiu7Cheuk-Lun Lee8Guangzhou Women and Children’s Medical Center, Institute of Pediatrics, Guangzhou, ChinaDepartment of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong SAR, ChinaDepartment of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong SAR, ChinaDepartment of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong SAR, ChinaDepartment of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong SAR, ChinaShenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong – Shenzhen Hospital, Shenzhen, ChinaDepartment of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong SAR, ChinaShenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong – Shenzhen Hospital, Shenzhen, ChinaDepartment of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, ChinaExtracellular vesicles (EVs) are cell-derived, membrane-bound vesicles that carry molecular cargo to facilitate communication between cells. During pregnancy, EVs are secreted by the syncytiotrophoblast layer of the placenta villi, where they mediate the functions of resident leukocytes and invading extravillous trophoblasts (EVTs) in the decidua. This study aims to isolate placental EVs (pEVs) from placental explant to examine their regulatory roles on EVT and endothelial cell functions. pEVs were successfully isolated from ex vivo cultured placental explant, which were capable to be internalized by EVTs and endothelial cells. pEVs stimulated the differentiation of trophoblast stem cells (TSCs) and enhanced the migration and invasion abilities of EVTs via CD147 receptor. Conversely, pEVs inhibited the tube formation ability and interleukin-6 (IL-6) secretion of endothelial cells. Together, these findings partially elucidate the role of pEVs during early pregnancy establishment, which may provide insights into pregnancy-related disorders.https://www.frontiersin.org/articles/10.3389/fcell.2025.1528714/fullextracellular vesicletrophoblastendothelial cellsspiral artery remodelingCD147pregnancy |
| spellingShingle | Kunfeng Bai Kunfeng Bai Xintong Li Yanjie Guo Ye Shang Leqian Lin Philip C. N. Chiu Philip C. N. Chiu Cheuk-Lun Lee The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions Frontiers in Cell and Developmental Biology extracellular vesicle trophoblast endothelial cells spiral artery remodeling CD147 pregnancy |
| title | The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions |
| title_full | The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions |
| title_fullStr | The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions |
| title_full_unstemmed | The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions |
| title_short | The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions |
| title_sort | regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions |
| topic | extracellular vesicle trophoblast endothelial cells spiral artery remodeling CD147 pregnancy |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1528714/full |
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