Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model
Cervical cancer poses significant clinical challenges, particularly in advanced stages. This study explores the therapeutic potential of andrographolide (AND), a bioactive compound derived from Andrographis paniculata, in mitigating cervical cancer progression using the chick embryo chorioallantoic...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2025-02-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11432 |
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| author | Wanwan Zou Jun Lou Yun Yi Yiming Cui Xiaoyan Chu |
| author_facet | Wanwan Zou Jun Lou Yun Yi Yiming Cui Xiaoyan Chu |
| author_sort | Wanwan Zou |
| collection | DOAJ |
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Cervical cancer poses significant clinical challenges, particularly in advanced stages. This study explores the therapeutic potential of andrographolide (AND), a bioactive compound derived from Andrographis paniculata, in mitigating cervical cancer progression using the chick embryo chorioallantoic membrane patient-derived xenograft (CAM-PDX) model. The model was validated through hematoxylin-eosin staining and immunohistochemistry, which confirmed its ability to accurately replicate the histological and molecular characteristics of patient-derived xenografts, establishing its reliability for therapeutic screening. A dose of 20 mg/kg AND was selected for further evaluation based on preliminary CAM assay findings. In the CAM-PDX model, AND significantly inhibited tumor growth, primarily by reducing angiogenesis and vessel density. Immunohistochemical analysis revealed that AND downregulated key proteins associated with cancer cell proliferation and survival, including Ki67, B-cell lymphoma 2 (BCL-2), and Erythroblast transformation-specific-related gene (ERG). These results indicate that AND not only disrupts tumor angiogenesis but also induces cell cycle arrest and promotes apoptosis in cervical cancer cells. In summary, this study successfully established a reproducible CAM-PDX model for drug evaluation and highlighted the potential of AND as a promising therapeutic candidate for cervical cancer, warranting further clinical investigation.
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| format | Article |
| id | doaj-art-f7b497495f724dd395047aae5a9e504a |
| institution | Kabale University |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj-art-f7b497495f724dd395047aae5a9e504a2025-02-11T16:43:08ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2025-02-0110.17305/bb.2025.11432Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX modelWanwan Zou0Jun Lou1Yun Yi2Yiming Cui3Xiaoyan Chu4https://orcid.org/0009-0000-7258-6176Jiangxi Provincial Cancer HospitalDepartment of Gynecological Oncology, Jiangxi Provincial Cancer Hospital, Nanchang, ChinaDepartment of Gynecological Oncology, Jiangxi Provincial Cancer Hospital, Nanchang, ChinaDepartment of Mathematics, Applied Mathematics and Statistics, Case Western Reserve University, Cleveland, USADepartment of Gynecological Oncology, Jiangxi Provincial Cancer Hospital, Nanchang, China Cervical cancer poses significant clinical challenges, particularly in advanced stages. This study explores the therapeutic potential of andrographolide (AND), a bioactive compound derived from Andrographis paniculata, in mitigating cervical cancer progression using the chick embryo chorioallantoic membrane patient-derived xenograft (CAM-PDX) model. The model was validated through hematoxylin-eosin staining and immunohistochemistry, which confirmed its ability to accurately replicate the histological and molecular characteristics of patient-derived xenografts, establishing its reliability for therapeutic screening. A dose of 20 mg/kg AND was selected for further evaluation based on preliminary CAM assay findings. In the CAM-PDX model, AND significantly inhibited tumor growth, primarily by reducing angiogenesis and vessel density. Immunohistochemical analysis revealed that AND downregulated key proteins associated with cancer cell proliferation and survival, including Ki67, B-cell lymphoma 2 (BCL-2), and Erythroblast transformation-specific-related gene (ERG). These results indicate that AND not only disrupts tumor angiogenesis but also induces cell cycle arrest and promotes apoptosis in cervical cancer cells. In summary, this study successfully established a reproducible CAM-PDX model for drug evaluation and highlighted the potential of AND as a promising therapeutic candidate for cervical cancer, warranting further clinical investigation. https://www.bjbms.org/ojs/index.php/bjbms/article/view/11432Cervical cancerandrographolideANDangiogenesis inhibitionchorioallantoic membrane patient-derived xenograftCAM-PDX |
| spellingShingle | Wanwan Zou Jun Lou Yun Yi Yiming Cui Xiaoyan Chu Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model Biomolecules & Biomedicine Cervical cancer andrographolide AND angiogenesis inhibition chorioallantoic membrane patient-derived xenograft CAM-PDX |
| title | Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model |
| title_full | Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model |
| title_fullStr | Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model |
| title_full_unstemmed | Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model |
| title_short | Andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a CAM-PDX model |
| title_sort | andrographolide suppresses cervical cancer progression by targeting angiogenesis and inducing apoptosis in a cam pdx model |
| topic | Cervical cancer andrographolide AND angiogenesis inhibition chorioallantoic membrane patient-derived xenograft CAM-PDX |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11432 |
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