Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy
Discectomy is a widely utilized approach for alleviating disc herniation; however, effective repair of postoperative annulus fibrosus (AF) defects remains a significant challenge. This study introduces a hydrogel patch with enhanced mechanical properties for AF repair fabricated using chitin (Ch), c...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259000642500095X |
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| author | Mingzhi Liu Zhiyong Cui Derong Xu Chenguang Liu Chuanli Zhou |
| author_facet | Mingzhi Liu Zhiyong Cui Derong Xu Chenguang Liu Chuanli Zhou |
| author_sort | Mingzhi Liu |
| collection | DOAJ |
| description | Discectomy is a widely utilized approach for alleviating disc herniation; however, effective repair of postoperative annulus fibrosus (AF) defects remains a significant challenge. This study introduces a hydrogel patch with enhanced mechanical properties for AF repair fabricated using chitin (Ch), collagen (Col), and chitin nanocrystals (ChNCs) through a freeze-thaw cycling technique. The Ch and Col components constitute the matrix of the hydrogel patch, while uniformly dispersed ChNCs act as a nanofiller, markedly improving the mechanical performance (compression strain: 95 %; compression modulus: 0.27 MPa) of the resulting Ch/Col@ChNCs hydrogel patch. The patch demonstrates advantageous properties, including high porosity, superior water absorption, thermal stability, and biodegradability in simulated body fluid. In vitro assessments reveal excellent biocompatibility with AF cells and enhanced collagen deposition. Furthermore, in vivo studies confirm that the patch effectively repairs postoperative disc defects, exhibiting strong integration with surrounding tissues and facilitating the orderly regeneration of fibrous tissue. This innovative hydrogel patch, combining exceptional properties with a straightforward fabrication process, presents a viable strategy for advancing clinical biomaterials for postoperative AF repair. |
| format | Article |
| id | doaj-art-f82858e1ca8f4cf698a642b1e327c00a |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-f82858e1ca8f4cf698a642b1e327c00a2025-02-12T05:31:41ZengElsevierMaterials Today Bio2590-00642025-04-0131101537Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomyMingzhi Liu0Zhiyong Cui1Derong Xu2Chenguang Liu3Chuanli Zhou4The Affiliated Hospital of Qingdao University, 266035, Qingdao, ChinaCollege of Marine Life Sciences, Ocean University of China, 266003, Qingdao, ChinaThe Affiliated Hospital of Qingdao University, 266035, Qingdao, ChinaCollege of Marine Life Sciences, Ocean University of China, 266003, Qingdao, China; Corresponding author.The Affiliated Hospital of Qingdao University, 266035, Qingdao, China; Corresponding author.Discectomy is a widely utilized approach for alleviating disc herniation; however, effective repair of postoperative annulus fibrosus (AF) defects remains a significant challenge. This study introduces a hydrogel patch with enhanced mechanical properties for AF repair fabricated using chitin (Ch), collagen (Col), and chitin nanocrystals (ChNCs) through a freeze-thaw cycling technique. The Ch and Col components constitute the matrix of the hydrogel patch, while uniformly dispersed ChNCs act as a nanofiller, markedly improving the mechanical performance (compression strain: 95 %; compression modulus: 0.27 MPa) of the resulting Ch/Col@ChNCs hydrogel patch. The patch demonstrates advantageous properties, including high porosity, superior water absorption, thermal stability, and biodegradability in simulated body fluid. In vitro assessments reveal excellent biocompatibility with AF cells and enhanced collagen deposition. Furthermore, in vivo studies confirm that the patch effectively repairs postoperative disc defects, exhibiting strong integration with surrounding tissues and facilitating the orderly regeneration of fibrous tissue. This innovative hydrogel patch, combining exceptional properties with a straightforward fabrication process, presents a viable strategy for advancing clinical biomaterials for postoperative AF repair.http://www.sciencedirect.com/science/article/pii/S259000642500095XHydrogel patchAnnulus fibrosus defect repairChitinCollagenChitin nanocrystal |
| spellingShingle | Mingzhi Liu Zhiyong Cui Derong Xu Chenguang Liu Chuanli Zhou Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy Materials Today Bio Hydrogel patch Annulus fibrosus defect repair Chitin Collagen Chitin nanocrystal |
| title | Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy |
| title_full | Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy |
| title_fullStr | Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy |
| title_full_unstemmed | Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy |
| title_short | Chitin nanocrystal-reinforced chitin/collagen composite hydrogels for annulus fibrosus repair after discectomy |
| title_sort | chitin nanocrystal reinforced chitin collagen composite hydrogels for annulus fibrosus repair after discectomy |
| topic | Hydrogel patch Annulus fibrosus defect repair Chitin Collagen Chitin nanocrystal |
| url | http://www.sciencedirect.com/science/article/pii/S259000642500095X |
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