Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved]
Background A novel modality of cancer treatment based on exposure to non-contact electric fields called Electro-Capacitive Cancer Therapy (ECCT) has been developed. However, the effects of this modality on vital organs during cancer treatment have not been fully investigated. Therefore, this study a...
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F1000 Research Ltd
2025-02-01
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| author | Luthfi Nurhidayat Ahmad Ghitha Fadhlurrahman Firman Alamsyah Warsito Purwo Taruno Nisrina Firdausi Subekti Evi Dwi Nugraheni Rarastoeti Pratiwi |
| author_facet | Luthfi Nurhidayat Ahmad Ghitha Fadhlurrahman Firman Alamsyah Warsito Purwo Taruno Nisrina Firdausi Subekti Evi Dwi Nugraheni Rarastoeti Pratiwi |
| author_sort | Luthfi Nurhidayat |
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| description | Background A novel modality of cancer treatment based on exposure to non-contact electric fields called Electro-Capacitive Cancer Therapy (ECCT) has been developed. However, the effects of this modality on vital organs during cancer treatment have not been fully investigated. Therefore, this study aimed to investigate the effects of non-contact electric field exposure on kidney and liver structures. Methods Female rats were randomly divided into one control group and three treatment groups with six replications each. Animals were treated with 7,12-dimethylbenz[a]anthracene at a dose of 20 mg/kg body weight for mammary tumour induction. Animals were then exposed to electric fields (100 kHz, 50-60 V/m) for 10 hours a day for three weeks. Two kidney samples and two liver samples from different animals in each group were collected for observation of structural damage to the organs. Histopathological cross-sections of the kidneys and livers were made using the paraffin method and Hematoxylin-Eosin staining. Histological scoring used the post-examination masking method with 100 visual fields per group. Results There was no significant damages to the tubules, glomeruli, and interstitial of the kidneys, including congestion, after exposure to non-contact electric fields. In addition, healthy rats exposed to this electric field showed significantly lower renal interstitial damage. There was no significant cellular damage, congestion, and haemorrhage in the livers of all groups, except in the healthy rat group that showed significantly higher haemorrhage. Conclusions Exposure to non-contact electric fields may cause haemorrhage in the livers of healthy rats. However, in kidney tissue, exposure to this electric field was tolerable, and can even decrease the number of inflammations and haemorrhages in healthy rats. |
| format | Article |
| id | doaj-art-f94e7719154c497db40429468f7e5786 |
| institution | Kabale University |
| issn | 2046-1402 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj-art-f94e7719154c497db40429468f7e57862025-02-12T01:00:01ZengF1000 Research LtdF1000Research2046-14022025-02-0112177801Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved]Luthfi Nurhidayat0https://orcid.org/0000-0002-0625-2984Ahmad Ghitha Fadhlurrahman1https://orcid.org/0000-0002-1239-8349Firman Alamsyah2https://orcid.org/0000-0002-7771-5026Warsito Purwo Taruno3Nisrina Firdausi4https://orcid.org/0000-0002-1799-1336Subekti Evi Dwi Nugraheni5https://orcid.org/0000-0001-5688-7024Rarastoeti Pratiwi6https://orcid.org/0000-0002-8421-2056Faculty of Biology, Universitas Gadjah Mada, Sleman, DI Yogyakarta, 55281, IndonesiaFaculty of Biology, Universitas Gadjah Mada, Sleman, DI Yogyakarta, 55281, IndonesiaFaculty of Science and Technology, Universitas Al-Azhar Indonesia, Jl. Sisingamangaraja, Jakarta, 12110, IndonesiaCenter for Medical Physics and Cancer Research, Ctech Labs Edwar Technology, Tangerang, Banten, 15143, IndonesiaResearch Center for Pharmaceutical Ingridients and Traditional Medicine, National Research and Innovation Agency Republic of Indonesia, Jl. Raya Jakarta-Bogor Km. 46, Cibinong West Java, 16911, IndonesiaMedical Laboratory, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudhi No. 229, Bandung West Java, 40154, IndonesiaFaculty of Biology, Universitas Gadjah Mada, Sleman, DI Yogyakarta, 55281, IndonesiaBackground A novel modality of cancer treatment based on exposure to non-contact electric fields called Electro-Capacitive Cancer Therapy (ECCT) has been developed. However, the effects of this modality on vital organs during cancer treatment have not been fully investigated. Therefore, this study aimed to investigate the effects of non-contact electric field exposure on kidney and liver structures. Methods Female rats were randomly divided into one control group and three treatment groups with six replications each. Animals were treated with 7,12-dimethylbenz[a]anthracene at a dose of 20 mg/kg body weight for mammary tumour induction. Animals were then exposed to electric fields (100 kHz, 50-60 V/m) for 10 hours a day for three weeks. Two kidney samples and two liver samples from different animals in each group were collected for observation of structural damage to the organs. Histopathological cross-sections of the kidneys and livers were made using the paraffin method and Hematoxylin-Eosin staining. Histological scoring used the post-examination masking method with 100 visual fields per group. Results There was no significant damages to the tubules, glomeruli, and interstitial of the kidneys, including congestion, after exposure to non-contact electric fields. In addition, healthy rats exposed to this electric field showed significantly lower renal interstitial damage. There was no significant cellular damage, congestion, and haemorrhage in the livers of all groups, except in the healthy rat group that showed significantly higher haemorrhage. Conclusions Exposure to non-contact electric fields may cause haemorrhage in the livers of healthy rats. However, in kidney tissue, exposure to this electric field was tolerable, and can even decrease the number of inflammations and haemorrhages in healthy rats.https://f1000research.com/articles/12-117/v6damages histology kidney liver non-contact electric field ECCTeng |
| spellingShingle | Luthfi Nurhidayat Ahmad Ghitha Fadhlurrahman Firman Alamsyah Warsito Purwo Taruno Nisrina Firdausi Subekti Evi Dwi Nugraheni Rarastoeti Pratiwi Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] F1000Research damages histology kidney liver non-contact electric field ECCT eng |
| title | Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] |
| title_full | Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] |
| title_fullStr | Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] |
| title_full_unstemmed | Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] |
| title_short | Effects of non-contact electric fields on the kidneys and livers of tumour-bearing rats [version 6; peer review: 1 approved, 2 approved with reservations, 1 not approved] |
| title_sort | effects of non contact electric fields on the kidneys and livers of tumour bearing rats version 6 peer review 1 approved 2 approved with reservations 1 not approved |
| topic | damages histology kidney liver non-contact electric field ECCT eng |
| url | https://f1000research.com/articles/12-117/v6 |
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