Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour
Three-dimensional (3D) scaffolds represent a transformative tool for studying physiological processes in health and disease contexts by mimicking in vivo conditions that two-dimensional (2D) culture systems cannot capture. This study explores the application of extrusion-based 3D bioprinting technol...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
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| Series: | Virtual and Physical Prototyping |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17452759.2025.2459802 |
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| Summary: | Three-dimensional (3D) scaffolds represent a transformative tool for studying physiological processes in health and disease contexts by mimicking in vivo conditions that two-dimensional (2D) culture systems cannot capture. This study explores the application of extrusion-based 3D bioprinting technology to create complex scaffolds mimicking the microenvironment of senescent cancer cells induced by radiotherapy. Using a viscoelastic hydrogel bioink composed of a blend of alginate and gelatin, we fabricated scaffolds with porosity and mechanical properties appropriate for culturing lung cells. We then delve into co-culture experiments to understand the cell-cell interactions between spatially patterned populations of normal bronchial epithelial cells (BEAS-2B) and senescent or non-senescent bronchial adenocarcinomic epithelial cells (A549). Normal epithelial cells exhibited non-professional phagocytic activity toward the cancer cells, markedly enhanced when senescence was induced in the cancer cells upon irradiation. As interest in non-animal models grows, these findings underscore the potential of 3D bioprinted platforms to serve as robust alternatives for preclinical research.Created with BioRender.com |
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| ISSN: | 1745-2759 1745-2767 |