Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour
Three-dimensional (3D) scaffolds represent a transformative tool for studying physiological processes in health and disease contexts by mimicking in vivo conditions that two-dimensional (2D) culture systems cannot capture. This study explores the application of extrusion-based 3D bioprinting technol...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Virtual and Physical Prototyping |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17452759.2025.2459802 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1825208906940416000 |
|---|---|
| author | Parul Yadav Pritiranjan Mondal Sibani Jani Deepak K. Saini Kaushik Chatterjee |
| author_facet | Parul Yadav Pritiranjan Mondal Sibani Jani Deepak K. Saini Kaushik Chatterjee |
| author_sort | Parul Yadav |
| collection | DOAJ |
| description | Three-dimensional (3D) scaffolds represent a transformative tool for studying physiological processes in health and disease contexts by mimicking in vivo conditions that two-dimensional (2D) culture systems cannot capture. This study explores the application of extrusion-based 3D bioprinting technology to create complex scaffolds mimicking the microenvironment of senescent cancer cells induced by radiotherapy. Using a viscoelastic hydrogel bioink composed of a blend of alginate and gelatin, we fabricated scaffolds with porosity and mechanical properties appropriate for culturing lung cells. We then delve into co-culture experiments to understand the cell-cell interactions between spatially patterned populations of normal bronchial epithelial cells (BEAS-2B) and senescent or non-senescent bronchial adenocarcinomic epithelial cells (A549). Normal epithelial cells exhibited non-professional phagocytic activity toward the cancer cells, markedly enhanced when senescence was induced in the cancer cells upon irradiation. As interest in non-animal models grows, these findings underscore the potential of 3D bioprinted platforms to serve as robust alternatives for preclinical research.Created with BioRender.com |
| format | Article |
| id | doaj-art-fd52b1a28603465daa6292fe8cb869eb |
| institution | Kabale University |
| issn | 1745-2759 1745-2767 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virtual and Physical Prototyping |
| spelling | doaj-art-fd52b1a28603465daa6292fe8cb869eb2025-02-06T19:57:44ZengTaylor & Francis GroupVirtual and Physical Prototyping1745-27591745-27672025-12-0120110.1080/17452759.2025.2459802Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumourParul Yadav0Pritiranjan Mondal1Sibani Jani2Deepak K. Saini3Kaushik Chatterjee4Department of Bioengineering, Indian Institute of Science, Bangalore, IndiaDepartment of Materials Engineering, Indian Institute of Science, Bangalore, IndiaDepartment of Bioengineering, Indian Institute of Science, Bangalore, IndiaDepartment of Bioengineering, Indian Institute of Science, Bangalore, IndiaDepartment of Bioengineering, Indian Institute of Science, Bangalore, IndiaThree-dimensional (3D) scaffolds represent a transformative tool for studying physiological processes in health and disease contexts by mimicking in vivo conditions that two-dimensional (2D) culture systems cannot capture. This study explores the application of extrusion-based 3D bioprinting technology to create complex scaffolds mimicking the microenvironment of senescent cancer cells induced by radiotherapy. Using a viscoelastic hydrogel bioink composed of a blend of alginate and gelatin, we fabricated scaffolds with porosity and mechanical properties appropriate for culturing lung cells. We then delve into co-culture experiments to understand the cell-cell interactions between spatially patterned populations of normal bronchial epithelial cells (BEAS-2B) and senescent or non-senescent bronchial adenocarcinomic epithelial cells (A549). Normal epithelial cells exhibited non-professional phagocytic activity toward the cancer cells, markedly enhanced when senescence was induced in the cancer cells upon irradiation. As interest in non-animal models grows, these findings underscore the potential of 3D bioprinted platforms to serve as robust alternatives for preclinical research.Created with BioRender.comhttps://www.tandfonline.com/doi/10.1080/17452759.2025.2459802Bioprintingsenescencecancerhydrogel scaffoldstissue models |
| spellingShingle | Parul Yadav Pritiranjan Mondal Sibani Jani Deepak K. Saini Kaushik Chatterjee Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour Virtual and Physical Prototyping Bioprinting senescence cancer hydrogel scaffolds tissue models |
| title | Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour |
| title_full | Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour |
| title_fullStr | Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour |
| title_full_unstemmed | Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour |
| title_short | Non-professional phagocytosis of senescent cancer cells revealed in a 3D-bioprinted model of an irradiated lung tumour |
| title_sort | non professional phagocytosis of senescent cancer cells revealed in a 3d bioprinted model of an irradiated lung tumour |
| topic | Bioprinting senescence cancer hydrogel scaffolds tissue models |
| url | https://www.tandfonline.com/doi/10.1080/17452759.2025.2459802 |
| work_keys_str_mv | AT parulyadav nonprofessionalphagocytosisofsenescentcancercellsrevealedina3dbioprintedmodelofanirradiatedlungtumour AT pritiranjanmondal nonprofessionalphagocytosisofsenescentcancercellsrevealedina3dbioprintedmodelofanirradiatedlungtumour AT sibanijani nonprofessionalphagocytosisofsenescentcancercellsrevealedina3dbioprintedmodelofanirradiatedlungtumour AT deepakksaini nonprofessionalphagocytosisofsenescentcancercellsrevealedina3dbioprintedmodelofanirradiatedlungtumour AT kaushikchatterjee nonprofessionalphagocytosisofsenescentcancercellsrevealedina3dbioprintedmodelofanirradiatedlungtumour |