Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing

Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing

Summary: Cap analysis of gene expression (CAGE) is a technique that facilitates the assessment of the 5′-end of RNA transcript starting site (TSS) of both coding and non-coding genes. Here, we present a protocol for using CAGE on Illumina patterned flow cell technology with dual indexes on mouse and...

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Bibliographic Details
Main Authors: Diane Delobel, Hiromi Nishiyori-Sueki, Ilaria Nisoli, Hideya Kawaji, Pauline Robbe, Piero Carninci, Hazuki Takahashi
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
sequencing
RNA-seq
gene expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007597
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