Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection
Background: The spectrum of dengue infection ranges from asymptomatic or mild to severe disease. The pathogenic mechanisms are not fully understood. A viral infection can induce the neutrophil extracellular traps (NETs), and the excessive NETs lead to increased vascular permeability, coagulopathy,...
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Tehran University of Medical Sciences
2024-08-01
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| Series: | Journal of Arthropod-Borne Diseases |
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| Online Access: | https://jad.tums.ac.ir/index.php/jad/article/view/1632 |
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| author | Cucunawangsih Cucunawangsih Ratna Sari Wijaya Nata Pratama Hardjo Lugito Ivet Suriapranata |
| author_facet | Cucunawangsih Cucunawangsih Ratna Sari Wijaya Nata Pratama Hardjo Lugito Ivet Suriapranata |
| author_sort | Cucunawangsih Cucunawangsih |
| collection | DOAJ |
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Background: The spectrum of dengue infection ranges from asymptomatic or mild to severe disease. The pathogenic mechanisms are not fully understood. A viral infection can induce the neutrophil extracellular traps (NETs), and the excessive NETs lead to increased vascular permeability, coagulopathy, and platelet dysfunction, a hallmark of severe dengue.
Methods: To evaluate the association of NETs formation with disease severity using a human public transcriptomic dataset (GSE17924) and clinical samples from dengue patients with different disease severity.
Results: Based on the transcriptomic analysis, the whole blood gene expression functional in neutrophil activities and NETs formation was upregulated with dengue disease severity. The serum concentration of citrullinated histone H3 (CitH3), a NETs marker, was measured in 28 dengue patients, of whom 18 classified as dengue fever (DF) and 10 as dengue hemorrhagic fever (DHF) grade 1 and 2. A significantly higher CitH3 concentration was found in DHF compared to DF patients. The level of CitH3 was negatively correlated with platelet counts.
Conclusion: Our results suggest NETs have contributed to the disease severity of dengue infection. Future studies on the predictive value of NETs markers and the potential NETs as a targeted therapy in dengue disease should be prioritized.
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| format | Article |
| id | doaj-art-fe72ce5df61b49198792c75536f5ec25 |
| institution | Kabale University |
| issn | 2322-1984 2322-2271 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Arthropod-Borne Diseases |
| spelling | doaj-art-fe72ce5df61b49198792c75536f5ec252025-02-09T09:05:07ZengTehran University of Medical SciencesJournal of Arthropod-Borne Diseases2322-19842322-22712024-08-0118210.18502/jad.v18i2.17540Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus InfectionCucunawangsih Cucunawangsih0Ratna Sari Wijaya1Nata Pratama Hardjo Lugito2Ivet Suriapranata3Department of Microbiology, Faculty of Medicine, Pelita Harapan University, Tangerang, IndonesiaDepartment of Microbiology, Faculty of Medicine, Pelita Harapan University, Tangerang, IndonesiaDepartment of Internal Medicine, Faculty of Medicine, Pelita Harapan University, Tangerang, IndonesiaDivision of Immunology, Mochtar Riady Institute for Nanotechnology and Medical Science Group, Pelita Harapan University, Tangerang, Indonesia Background: The spectrum of dengue infection ranges from asymptomatic or mild to severe disease. The pathogenic mechanisms are not fully understood. A viral infection can induce the neutrophil extracellular traps (NETs), and the excessive NETs lead to increased vascular permeability, coagulopathy, and platelet dysfunction, a hallmark of severe dengue. Methods: To evaluate the association of NETs formation with disease severity using a human public transcriptomic dataset (GSE17924) and clinical samples from dengue patients with different disease severity. Results: Based on the transcriptomic analysis, the whole blood gene expression functional in neutrophil activities and NETs formation was upregulated with dengue disease severity. The serum concentration of citrullinated histone H3 (CitH3), a NETs marker, was measured in 28 dengue patients, of whom 18 classified as dengue fever (DF) and 10 as dengue hemorrhagic fever (DHF) grade 1 and 2. A significantly higher CitH3 concentration was found in DHF compared to DF patients. The level of CitH3 was negatively correlated with platelet counts. Conclusion: Our results suggest NETs have contributed to the disease severity of dengue infection. Future studies on the predictive value of NETs markers and the potential NETs as a targeted therapy in dengue disease should be prioritized. https://jad.tums.ac.ir/index.php/jad/article/view/1632Neutrophil extracellular traps; NETs; Neutrophil; Dengue |
| spellingShingle | Cucunawangsih Cucunawangsih Ratna Sari Wijaya Nata Pratama Hardjo Lugito Ivet Suriapranata Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection Journal of Arthropod-Borne Diseases Neutrophil extracellular traps; NETs; Neutrophil; Dengue |
| title | Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection |
| title_full | Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection |
| title_fullStr | Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection |
| title_full_unstemmed | Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection |
| title_short | Neutrophil Extracellular Traps Contribute to the Disease Severity of Dengue Virus Infection |
| title_sort | neutrophil extracellular traps contribute to the disease severity of dengue virus infection |
| topic | Neutrophil extracellular traps; NETs; Neutrophil; Dengue |
| url | https://jad.tums.ac.ir/index.php/jad/article/view/1632 |
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