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181
Surface plasmon resonance biosensors for SARS-CoV-2 sensing: The role of silicon nitride and graphene
Published 2025-05-01“…Sys₃, optimized with Ag at 55 nm, Si₃N₄ at 13 nm, and ssDNA at 10 nm, demonstrated exceptional sensitivity (371.7°/RIU), low limit of detection, and high detection accuracy, making it suitable for precision applications. …”
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182
Hybrid and/or mixed infection by pig and human Ascaris in a Guarani indigenous village in southern Brazil.
Published 2025-02-01“…The soil, which was contaminated by both human and swine feces, also contained eggs of the two Ascaris species and hybrids, thus constituting a source of Ascaris infection for both hosts. DNA from A. lumbricoides and A. suum, individually, was detected in samples from humans and pigs, respectively, and the soil, while DNA from hybrid and/or Ascaris spp. was detected in samples from both hosts and the soil.…”
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183
The effect of bacterial overgrowth syndrome and bacterial translocation on the course of liver cirrhosis
Published 2012-09-01“…To determine the effect of bacterial overgrowth syndrome and translocation of bacterial DNA in ascitic fluid on morbidity in liver cirrhosis (LC) and long-term life expectancy.Material and methods. …”
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184
A universal method for the purification of C2H2 zinc finger arrays.
Published 2025-01-01“…Zinc fingers (ZFs) are compact, modular, sequence-specific polynucleotide-binding domains uniquely suited for use as DNA probes and for the targeted delivery of effector domains for purposes such as gene regulation and editing. …”
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185
Three new species of Mesobiotus (Eutardigrada: Macrobiotidae) from Sweden with an updated phylogeny of the genus
Published 2025-02-01“…All three species are distinguished morphologically and through differences in DNA sequences as supported by PTP and mPTP analyses. …”
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186
Dimerization-dependent serine protease activity of FAM111A prevents replication fork stalling at topoisomerase 1 cleavage complexes
Published 2024-03-01“…Dimerization is essential for proteolytic activity in vitro and for facilitating DNA replication at DNA-protein crosslink obstacles in cells, while it is dispensable for autocleavage. …”
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187
Functional analysis of a novel homozygous missense IVD gene variant: a case report with dual genetic diagnoses
Published 2025-02-01“…Functional analysis of the IVD variant in vitro was performed. Mutant IVD gene pcDNA3.1(+)-MUT-3xFlag and control pcDNA3.1(+)-WT-3xFlag mammalian expression vectors were constructed. …”
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188
Impact of R-loops on oncogene-induced replication stress in cancer cells
Published 2023-09-01“…It is now well established that transcription can interfere with genome replication in different ways, such as head-on collisions between polymerases, accumulation of positive DNA supercoils or formation of R-loops. These structures form during transcription when nascent RNA reanneals with DNA behind the RNA polymerase, forming a stable DNA:RNA hybrid. …”
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189
Practical techniques in molecular biotechnology /
Published 2021Table of Contents: “…Recombinant DNA and protein technology -- Enzyme kinetics, proteomics and mass spectrometry -- Bioanalytical techniques -- Molecular biology -- Cell culture -- Antibody technology.…”
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190
Early tolerance and late persistence as alternative drug responses in cancer
Published 2025-02-01“…The central regulator of mitophagy PINK1 drives this reduction in DNA repair via the cytoplasmic relocalization of the cell identity master HNF4A, thus hampering HNF4A transcriptional activation of DNA repair genes. …”
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191
Frequency of BK virus genotypes in patients with colorectal cancer
Published 2025-02-01“…The detection of BK virus DNA VP1 gene and genotyping were carried out through nested-PCR and sequencing techniques. …”
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192
Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study
Published 2023-12-01“…No major differences were detected in the quantification of periodontopathogens evaluated between the control group and CP.ConclusionAdolescents with CP showed variability in the detection of DNA of periodontopathogens, especially a great variation in the detection of P gingivalis in saliva of CP with gingivitis.…”
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193
Museomics and morphological analyses of historical and contemporary peninsular Italian wolf (Canis lupus italicus) samples
Published 2025-02-01“…Therefore, in this study, we morphologically and genetically analyzed historical and contemporary wolf samples, also exploiting the optimization of an innovative bone DNA extraction method, to describe the morphological variability of the subspecies and its genetic diversity during the last 30 years. …”
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194
Taxonomic refinement of Bacillus thuringiensis
Published 2025-02-01“…This study investigates the taxonomic authenticity of those strains using DNA similarity indexes, i.e., average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH). …”
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195
Pancreas Proapoptotic Proteases in Patients with Chronic Pancreatitis
Published 2019-06-01“…To study features of localization of the DNA-ase I and endonuclease-G proapoptotic proteases in the pancreas in chronic pancreatitis (CP). …”
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196
A bidirectional Mendelian randomization study integrating genome-wide association studies, expression quantitative trait locus, and methylation quantitative trait locus data reveal...
Published 2025-02-01“…By incorporating consistent summary data-based Mendelian randomization associations between DNA methylation and smoke/Barrett’s esophagus, gene expression and smoke/Barrett’s esophagus, and DNA methylation and gene expression, we identified that the genetic variant-cg00935895-RBM43 (ENSG00000184898)-smoke/Barrett’s esophagus axis exerted an effect on smoke/Barrett’s esophagus by altering the DNA methylation level, which regulated the expression level of RBM43. …”
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197
A soft tick vector of Babesia sp. YLG in Yellow-legged gull (Larus michahellis) nests
Published 2023-09-01“…Parasite DNA was also found in tick ovaries, which could indicate possible transovarial transmission. …”
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198
Genomic data suggest parallel dental vestigialization within the xenarthran radiation
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199
Protracted HBsAg-aemia in a Patient with Acute Hepatitis B
Published 2018-11-01“…These methods allowed a protracted persistence of HBV DNA in the blood and a late seroconversion of HBsAg/anti-HBs to be revealed.Conclusion. …”
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200
New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
Published 2025-02-01“…The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. …”
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