Post-translational modifications as a key mechanism for herpes simplex virus type I evasion of host innate immunity by Yongxing Zhang, Junlei Xie, Ying Feng, Abdul Qadeer, Shanni Li, Xu Deng, Lipeng Zhu, Bo Kong, Zanxian Xia, Zanxian Xia

“…Herpes simplex virus type 1 (HSV-1) is a DNA virus that infects humans and establishes long-term latency within the host. …”
Get full text

Recent updates of centromere proteins in hepatocellular carcinoma: a review by Zhongyuan Yang, Wenjiao Chen, Yunhui Liu, Yuxin Niu

“…Centromeres are considered special deoxyribonucleic acid (DNA) sequences with highly repetitive sequences that are physically connected to the spindle during cell division, ensuring equal division of genetic material between daughter cells. …”
Get full text

Genes of Inflammation and Placental Function GWAS Associated with Idiopathic Recurrent Miscarriage in the Kazakh Population by Alexandra Murtazaliyeva, Gulnara Svyatova, Galina Berezina, Gulfairuz Urazbayeva, Aigerim Sadyrbekova

“…Materials and Methods: Molecular genetic studies were performed by TaqMan using a single site-specific amplification and real-time genotyping method in 302 women with iRM and 300 with normal reproduction. DNA isolation from the biomaterial was carried out using kits containing binding magnetic particles. …”
Get full text

A Nonsynonymous Single-nucleotide Polymorphism in SLC24A5 Regulates Feather Pigment Deposition in Chinese Yellow Quail by Xiaohui Zhang, Fanghu Wu, Yanxia Qi, Yuanyuan Shang, Lingyun Fan, Yifei Wang

“…The main objective of this study was to detect polymorphisms in the SLC24A5 gene of the Chinese yellow quail (Coturnix japonica) and analyze their effect on tyrosinase activity in skin tissue and melanin content in down feathers. The cDNA of the SLC24A5 gene was cloned by RT-PCR and subjected to Sanger sequencing. …”
Get full text

CTSG is a prognostic marker involved in immune infiltration and inhibits tumor progression though the MAPK signaling pathway in non-small cell lung cancer by Qian Dai, Xufeng Yao, Yanke Zhang, Qian Chai, Xueyi Feng, Hongbin Zhu, Lei Zhao

“…CTSG suppresses the MAPK and EMT signaling pathways in NSCLC cells. Moreover, DNA methylation and histone deacetylation have been identified as crucial mechanisms contributing to the decreased expression of CTSG. …”
Get full text

Conducting genetic epidemiology research on hyperuricaemia and gout in an indigenous community in Guam - a feasibility study by Yvette C. Paulino, Frank Camacho, Tristan VC Paulino, Delores J. Lee, Lisa Linda Natividad, Elizabeth Matisoo-Smith, Tony R. Merriman, Anna L. Gosling

“…Methods In this feasibility study, the University of Guam led the qualitative inquiry with the native community, training (pre-screening of participants, data collection methods, and biospecimen handling), study implementation (outreach and recruitment, data collection, and DNA extraction and quantification), and qualitative and epidemiologic data analyses. …”
Get full text

Prevalence of Erosive-ulcerative Lesions of the Stomach and Helicobacter Pylori Infection in Patients with Ulcerative Colitis by N. A. Ponkratova, P. V. Pavlov, O. S. Shifrin

“…All patients, along with the standard examination using esophagogastroduodenoscopy, were diagnosed with regard to H. pylori infection: all 70 patients received a rapid urease test (RUT), 24 patients (34.3 %) had H. pylori DNA analyzed in feces, and 46 patients (65.7 %) had 13C labeled urea breath test. …”
Get full text

Diversity of bacterial symbionts associated with the tropical plant bug Monalonion velezangeli (Hemiptera: Miridae) revealed by high-throughput 16S-rRNA sequencing by Navarro-Escalante, Lucio, Benavides, Pablo, Acevedo, Flor E.

“…Based on PCR screening and DNA sequencing of the wsp gene, Wolbachia infection was confirmed in almost 80% of samples, and represented by two different isolates or strains within the supergroup B. …”
Get full text

Structural basis of SIRT7 nucleosome engagement and substrate specificity by Carlos Moreno-Yruela, Babatunde E. Ekundayo, Polina N. Foteva, Dongchun Ni, Esther Calvino-Sanles, Henning Stahlberg, Beat Fierz

“…The catalytic domain binds at the H3-tail exit site, engaging both DNA gyres of the nucleosome. Contacting H3K36 versus H3K18 requires a change in binding pose, and results in structural changes in both SIRT7 and the nucleosome. …”
Get full text

Telomere length varies with sex, hatching rank and year of birth in the Little Owl, Athene noctua by Criscuolo, François, Fache, Inès, Scaar, Bertrand, Zahn, Sandrine, Bleu, Josefa

“…Telomeres are non-coding DNA sequences located at the end of linear chromosomes, protecting genome integrity. …”
Get full text

A descriptive-multivariate analysis of community knowledge, confidence, and trust in COVID-19 clinical trials amongst Ugandans working in healthcare settings. by Keneth Iceland, Kasozi, Lawrence Obado, Osuwat

“…KCTCOVacTrials were higher amongst the least educated (certificate holders) than bachelor degree holders. Skepticism against DNA recombinant vaccines (DRV) implies genetically modified vaccines such as the Oxford- AstraZeneca vaccine, Pfizer/BioNTech are bound to face a level of resistance once adapted in Uganda. …”
Get full text

Emerging Anthelmintic Resistance in Poultry: Can Ethnopharmacological Approaches Offer a Solution? by Zirintunda, Gerald, Biryomumaisho, Savino, Keneth Iceland, Kasozi, Batiha, Gaber El-Saber, Kateregga, John, Vudriko, Patrick, Nalule, Sarah, Olila, Deogracious, Mariam Kajoba, Mariam, Matama, Kevin, Kwizera, Mercy Rukundo, Ghoneim, Mohammed M., Abdelhamid, Mahmoud, Alshehri, Sultan, Abdelgawad, Mohamed A., Acai-Okwee, James, Zaghlool, Sameh S.

“…Benzyl isothiocyanate glucosinolates interfere with DNA replication and protein expression, while isoflavones from Acacia oxyphylla cause helminth flaccid paralysis, inhibit energy generation, and affect calcium utilization. …”
Get full text

Evaluation of dried blood spot sampling for real-time PCR malaria diagnostics in a rural setting in Angola by Alejandro Mediavilla, Begoña Febrer-Sendra, Aroa Silgado, Patricia Martínez-Vallejo, Beatriz Crego-Vicente, Arlette Nindia, Carles Rubio Maturana, Lidia Goterris, Joan Martínez-Campreciós, Sandra Aixut, Pedro Fernández-Soto, María Luisa Aznar, Antonio Muro, Inés Oliveira-Souto, Israel Molina, Elena Sulleiro

“…Conclusions The DBS volume used for DNA extraction is a determining factor in the performance of real-time PCR for Plasmodium DNA detection. …”
Get full text

Whole genomic analysis uncovers high genetic diversity of rifampicin-resistant Mycobacterium tuberculosis strains in Botswana by Tuelo Mogashoa, Tuelo Mogashoa, Johannes Loubser, Ontlametse T. Choga, Ontlametse T. Choga, Justice Tresor Ngom, Wonderful T. Choga, Wonderful T. Choga, Mpaphi B. Mbulawa, Tuduetso Molefi, One Stephen, Topo Makhondo, Kedumetse Seru, Patience Motshosi, Boitumelo Zuze, Joseph Makhema, Rosemary M. Musonda, Dimpho Otukile, Chawangwa Modongo, Botshelo T. Kgwaadira, Keabetswe Fane, Simani Gaseitsiwe, Rob M. Warren, Sikhulile Moyo, Sikhulile Moyo, Sikhulile Moyo, Sikhulile Moyo, Anzaan Dippenaar, Elizabeth M. Streicher

“…Library preparation was performed using the Illumina DNA prep kit following the manufacturer's instructions. …”
Get full text

The Role of Phosphatidylethanolamine-N-methyltransferase (PEMT) Gene rs12449964 Polymorphism in the Development of Acute Pancreatitis and its Complications by T. A. Samgina, Yu. E. Azarova, Yu. V. Kanishchev, V. A. Lazarenko, P. M. Nazarenko, A. V. Polonikov

“…The average age of patients and healthy individuals was 48.9 ± 13.1 and 47.89 ± 12.1 years, respectively. Genomic DNA was isolated by a standard phenol-chloroform extraction method. …”
Get full text

Association of HLA-B27 Gene and Rheumatoid Arthritis: Analysis of Potential Role as a Predictive Biomarker by Karzan Ghafur Khidhir, Baban Ahmad, Dana Khdr Sabir

“…Samples of human blood from RA patients and healthy individuals were collected, and RNA extraction, cDNA synthesis and qPCR were carried out to detect relative expression of HLA-B27. …”
Get full text

Identifying common pathways for doxorubicin and carfilzomib-induced cardiotoxicities: transcriptomic and epigenetic profiling by Conagh Kelly, Dylan J. Kiltschewskij, Angeline J.W. Leong, Tatt Jhong Haw, Amanda J. Croft, Lohis Balachandran, Dongqing Chen, Danielle R. Bond, Heather J. Lee, Murray J. Cairns, Aaron L. Sverdlov, Doan T. M. Ngo

“…Both Doxorubicin and Carfilzomib induced a significant reduction in HCM cell viability and cell damage. DNA methylation analysis performed using MethylationEPIC array showed distinct and common changes induced by Doxorubicin and Carfilzomib (10,270 or approximately 12.9% of the DMPs for either treatment overlapped). …”
Get full text

Relative expression of IL-10 and TNF-α mRNA of kidney and spleen tissues of rat with and without mammary tumor after exposed to alternating current electric field by Nafisa Khoirun Widiasri, Anysah Nur Fauziyah, Firman Alamsyah, Rarastoeti Pratiwi

“…Total RNA from the kidney and spleen was isolated, cDNA was synthesized by reverse transcription PCR, and analysis of IL-10 and TNF-α mRNA expression was performed by RT-qPCR method. …”
Get full text

Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apcmin/+ mice from wildtype mice. by Yuting Sun, Tingyu Zhou, Silin Ye, Effie Yin Tung Lau, Yao Zeng, Jessie Qiaoyi Liang

“…Dye-based qPCR and melting curve assays exhibited distinct dissociation patterns that differentiated between synthesised wildtype (TT) and heterozygous mutant (TA) DNA and between TT and TA genotype mice based on analysis of tissue samples. …”
Get full text

The knowledge mapping of traditional Chinese medicine for hepatocellular carcinoma: A bibliometric study by Wenya Pei, Boyu Jiao, Zexiong Chen, Jingchun Zeng, Yancheng Fu, Zhanmou Liang, Baoguo Sun, Jingwen Ruan, Shijun Zhang

“…The analysis indicated that traditional Chinese medicine may enhance immunity and regulate the local tumor microenvironment through mechanisms involving DNA damage, cell cycle arrest, and apoptosis by signaling pathways. …”
Get full text