Efficacy and safety of dexamethasone or triamcinolone in combination with anti-vascular endothelial growth factor therapy for diabetic macular edema: A systematic review and meta-a... by Bo Zhou, Hua Liu, Feng Xiong

“…The assessment of heterogeneity was conducted via Cochran's Q test, I2 statistics, and the implementation of a 95% prediction interval (PI). …”
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Histological and immune response in the fish Centropomus viridis elicited by the parasite Rhabdosynochus viridisi by Dania López-Moreno, Zeinab Yazdi, Francisco N. Morales-Serna, Juan M. Martínez-Brown, Leonardo Ibarra-Castro, Alejandra García-Gasca, Selene M. Abad-Rosales, Rodolfo Lozano-Olvera, Emma J. Fajer-Ávila, Esteban Soto

“…Histological alterations in gills were evaluated by the severity degree and the degree of tissue change. RT-qPCR assays were developed to investigate the expression of il1B, il8, il10, il12, il17, igM, igT, ifnγ, tnfα, tbet, hsp70, foxp3a, stat4, and cmip in gills and head kidney. …”
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METTL14-mediated m6A modification of DDIT4 promotes its mRNA stability in aging-related idiopathic pulmonary fibrosis by Dan Li, Li Qian, Yufeng Du, Lifang Liu, Ziyue Sun, Yongkang Han, Xiangrui Guo, Chao Shen, Zheng Zhang, Xuejun Liu

“…The expressions of identified m6A regulator and target gene were validated using MeRIP-qPCR and real-time PCR. Moreover, we established an animal model and a senescent model of A549 cells to explore the associated molecular mechanism. …”
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Modeling quinoa growth under saline and water-limiting conditions using SWAP-WOFOST by Diana C. Estrella Delgado, Tom De Swaef, Jan Vanderborght, Eric Laloy, Gerda Cnops, Maarten De Boever, Abdelaziz Hirich, Ayoub El Mouttaqi, Sarah Garré

“…Field experimental data were used from two quinoa varieties: ICBA-Q5 grown under saline conditions in Laayoune, Morocco, in 2021, and Bastille grown under rainfed, non-saline conditions in Merelbeke, Belgium, from 2018 to 2023. …”
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Folic acid supplementation during preconception period in sub-Saharan African countries: A systematic review and meta-analysis. by Mekuriaw Nibret Aweke, Elsa Awoke Fentie, Muluken Chanie Agimas, Lemlem Daniel Baffa, Ever Siyoum Shewarega, Aysheshim Kassahun Belew, Esmael Ali Muhammad, Berhanu Mengistu

“…Heterogeneity was evaluated with Cochrane Q and I2 statistics, and small study effects were tested with Egger's test at a 5% significance level. …”
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Exploring MAP3K genes in gastric cancer: biomarkers, tumor microenvironment dynamics, and chemotherapy resistance by Senhui Wei, Ying Li, Jing Zhou, Yongming Xia

“…Methods In this study, we investigated the expression profiles and clinical implications of MAP3K genes in GC using in silico and in vitro experiments. Results Utilizing RT-qPCR analysis, we observed significant up-regulation of MAP3K1, MAP3K4, MAP3K5, MAP3K6, MAP3K7, MAP3K8, MAP3K9, and MAP3K10 in GC cell lines, while MAP3K2, MAP3K3, MAP3K11, MAP3K12, MAP3K13, MAP3K14, and MAP3K15 exhibited down-regulation. …”
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Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration by Andrea Barragán-Cárdenas, Daniel Castellar-Almonacid, Yerly Vargas-Casanova, Claudia Parra-Giraldo, Adriana Umaña-Pérez, Joel López-Meza, Zuly Rivera-Monroy, Javier García-Castañeda

“…Evaluation of mechanism associated to the cytotoxic effect were carried out by flow cytometry, RT-qPCR, wound healing, transwell and zymography. …”
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Bioinformatics insights into mitochondrial and immune gene regulation in Alzheimer's disease by Tian Meng, Yazhou Zhang, Yuan Ye, Hui Li, Yongsheng He

“…After platform removal, there was a notable increase in the path complexity and time required to reach the target quadrant, suggesting a reduction in spatial memory capacity in AD mice. Moreover, qRT-PCR validation confirmed that the mRNA expression of the five biomarkers was consistent with bioinformatics results. …”
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PARALELO ENTRE LA ACTIVIDAD Y LA SUSTANCIA ADICTIVA DESDE UN ENFOQUE SOCIAL Y SUS POSIBLES TRATAMIENTOS by Alan Herzberg, Viviana Messina, Diego Moreno, Natalia Olmedo, Romina Pitronello, Francisca Roman

“…El tema de las adicciones ha sido abordado históric amente por la literatura desde múltiples puntos de vista, sin embargo en esta investigación bibliográfica nuestra intenci ón es dimensionar las teorías sobre el origen de la s adicciones, considerando la complementariedad teórica para la q ue se presta el tema, permitiendo un paralelo con l as terapias psicoanalíticas y la visión freudiana. …”
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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blotting were utilized to detect the ITGB2 expression. …”
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Mechanism of melanoma suppression by Prosopis juliflora derived alkaloids: Apoptosis induction and signaling pathway inhibition by Jasoda Choudhari, Snehal K. Nimal, Shridhar Chougule, Trupti Shinde, N.R. Dhatrak, Gopal C. Kundu, Rajesh N. Gacche

“…Mechanistic study of PJLME-AF was explored using ROS generation assay, apoptosis assays (Annexin V) assay, western blot (apoptotic protein, EMT markers, stem cell markers and signalling pathways), qPCR. In-vitro spheroid assay was performed to explored the PJLME-AF cytotoxic effects on tumour spheroid forming ability of melanoma cells. …”
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Comparison of phenotypic and genetic traits of ESBL-producing UPEC strains causing recurrent or single episode UTI in postmenopausal women by Michelle Kalu, Peter Jorth, Annie Wong-Beringer

“…Presence of hlyA, ompT, and fimH genes were confirmed by colony PCR, and qRT-PCR was performed using extracted RNA from a subset of 18 strains (12 recurrent; 6 single episode) grown in Luria-Bertani media and isolated from infected BECs to characterize gene expression. …”
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Targeting CXCL8 signaling sensitizes HNSCC to anlotinib by reducing tumor-associated macrophage-derived CLU by Xin Hu, Yikang Ji, Mi Zhang, Zhihui Li, Xinhua Pan, Zhen Zhang, Xu Wang

“…In vivo and in vitro CXCL8 and CLU expression levels in tumor cells were determined using qPCR, immunohistochemistry and ELISA. The ability of CLU from TAMs to respond to tumor-derived CXCL8 was assessed using RNA sequencing, siRNA silencing, immunofluorescence and CCK-8 assays. …”
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Emergency Physicians’ and Nurses’ Perspectives on Transgender, Intersexual, and Non-Binary Patients in Germany by Torben Brod, Carsten Stoetzer, Christoph Schroeder, Stephanie Stiel, Kambiz Afshar

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Influence of nano-polystyrene on cyfluthrin toxicity in honeybee Apis cerana cerana Fabricius by Yunfei Xue, Xueyang Gong, Linfu Yang, Dandan Zhi, Qingxin Meng, Yulong Guo, Kun Dong, Yakai Tian

“…Concerning the detoxification-related genes, the two toxins alone and in combination significantly promoted the expression of P450 9E2 and Cyp9Q3 genes, and the upregulation trend was found more significant for the combination. …”
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Vitamin B12, folate, and homocysteine levels in children and adolescents with obesity: a systematic review and meta-analysis by Juan R. Ulloque-Badaracco, Esteban A. Alarcon-Braga, Enrique A. Hernandez-Bustamante, Enrique A. Hernandez-Bustamante, Beatrix M. Von-Koeller-Jones, Miguel Huayta-Cortez, Esduardo Saavedra-Custodio, Percy Herrera-Añazco, Vicente A. Benites-Zapata

“…Standardized mean difference (SMD) with the corresponding 95% CI was used as the only effect size. The Cochran’s Q test and the I2 statistic were used to evaluate between-study heterogeneity. …”
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A systematic review on the correlation between COVID-19 and lower urinary tract symptoms by Ashkan Shafigh, Amir Mohammadi-Garebagh, Kavous Shahsavarinia, Sona Tayebi, Ali Mostafaei, Hanieh Salehi-Pourmehr, Sakineh Hajebrahimi

“…The analysis of eligible studies showed a 3.3 % prevalence of LUTS in infected patients (95 % CI: 2.0 % - 5.3 %; Q-value: 1021.397, I2: 97.45 %). Furthermore, frequency and urgency were the most prevalent symptoms in the eligible meta-analysis studies, with 15.3 % (95 % CI: 5.7 % – 34.9 % in 4 studies) and 11.5 % (95 % CI: 7.1 % - 18.1 % in two studies), respectively. …”
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Genomic analysis of the SMN1 gene region in patients with clinically diagnosed spinal muscular atrophy: a retrospective observational study by Tamaki Kato, Mamoru Yokomura, Yutaka Osawa, Kensuke Matsuo, Yuji Kubo, Taihei Homma, Kayoko Saito

“…Most patients with SMA have a mutation in the survival motor neuron 1 (SMN1) gene on chromosome 5q. With current genetic testing, SMN1 copy number is determined; a diagnosis is reached when the copy number is zero. …”
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Deciphering Regulatory Networks Governing Seedling Emergence in Deep-Sown Direct-Seeded Rice Cultivation by Jasneet Singh, Nitika Sandhu, Aman Kumar, Om Prakash Raigar, Sutej Bains, Gaurav Augustine, Muskan Gupta, Ekta Kharche, Anu Kalia, Navtej Singh Bains, Arvind Kumar

“…From this pool of DEGs, 24 genes were validated using qRT-PCR. Among these, two genes (LOC_Os04g51460 and LOC_Os02g45450) contribute to cell wall remodelling and membrane stability, while three genes (LOC_Os04g48484, LOC_Os06g04399, and LOC_Os07g15440) play key roles in mitigating abiotic stress. …”
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Efecto del nivel de suplementación con harina de algodón sobre el consumo y el aumento de peso en bovinos en recría alimentados con silaje de sorgo by Jose L. Chiossone, O. Balbuena

“….-1 difirió (p<0,05) con el aumento del nivel de suplementación, pero no se detectan diferencias (p=0,12) como g kg-1 PV, aunque en ambos casos se verificó un efecto cuadrático (Q<0,05). El tratamiento testigo (P0) presentó un ADPV de 0,07 kg an-1 .-1, con un CS de 18,2 g kg-1 PV que se incrementó 31,3% al incluir 3 g kg-1 PV de HA. …”
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