New nucleopolyhedrovirus isolate in the management of Spodoptera eridania by ALIXELHE P. DAMASCENA, FERNANDO Z. MADALON, LUIS M. DE ARAUJO JUNIOR, DIRCEU PRATISSOLI, CARLOS EDUARDO C. PAIVA, LORENA C. MACHADO, HUGO GONÇALVES JUNIOR, VINÍCIUS H. BELLO

“…Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. …”
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CoTF-reg reveals cooperative transcription factors in oligodendrocyte gene regulation using single-cell multi-omics by Jerome J. Choi, John Svaren, Daifeng Wang

“…We introduce coTF-reg, an analytical framework that integrates scRNA-seq and scATAC-seq data to identify cooperative TFs co-regulating the target gene (TG). …”
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In vivo regulation of the monocyte phenotype by Mycobacterium marinum and the ESX-1 type VII secretion system by Kristina Munke, Line Wulff, Julia Lienard, Fredric Carlsson, William W. Agace

“…In the current study we use this model, in combination with scRNA sequencing, to assess the impact of M. marinum infection on the transcriptional profile of infiltrating Ly6C⁺MHCII⁺ monocytes in vivo. …”
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Sarcoid-like reaction at the patient with chronic hepatitis C on a background of antiviral therapy by A. V. Lapshin, N. I. Kokina, M. V. Mayevskaya, I. A. Sokolina, V. T. Ivashkin

“…At female 53 year-old patient with chronic hepatitis C (HCV RNA+, genotype 1b) of moderate activity (А2, F0 by METAVIR scale) development of granulomatous pulmonary lesion accompanied by fever, respiratory failure, asthenic syndrome had been observed on the 22-nd week of antiviral therapy by PEG-interferon and ribavirin. …”
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The intestinal microbiome as a separate organ by Yu. O. Shulpekova

“…Analysis of sequential cloning of 16S ribosomal RNA possesses the highest information value of all up-to-date methods. …”
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Impaired cognitive function and decreased monoamine neurotransmitters in the DNAJC12 gene knockout mouse model by Shunan Wang, Ming Shen, Bo Pang, Bo Zhou, Yuan Yuan, Mei Lu, Xiangling Deng, Min Yang, Shufang Liu, Qiong Wang, Mei Xue, Qisheng Xia, Zhixin Zhang

“…To assess the impact of these mutations on DNAJC12 expression, we used quantitative real-time PCR and Western blotting techniques to measure mRNA and protein levels, respectively. The Morris water maze test was administered to evaluate the cognitive functions of the mice. …”
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An integrated microbiome- and metabolome-genome-wide association study reveals the role of heritable ruminal microbial carbohydrate metabolism in lactation performance in Holstein... by Chenguang Zhang, Huifeng Liu, Xingwei Jiang, Zhihong Zhang, Xinfeng Hou, Yue Wang, Dangdang Wang, Zongjun Li, Yangchun Cao, Shengru Wu, Sharon A. Huws, Junhu Yao

“…The identified SNPs of chr25:11,177 on 5s_rRNA (small ribosomal RNA) (P < 0.05/nSNPs) were linked to ECM, the abundance alteration of Pseudobutyrivibrio_sp. …”
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IL-6/GATA2/SERPINE1 pathway is implicated in regulating cellular senescence after acute kidney injury by Hongshuang Su, Xiaoxi Lin, Ayinuer Paredong, Ayinuer Paredong, Congcong Yao, Yan Zhang, Mengke Geng, Yuqian Guan, Lichao Gong, Feng Jiang, Qi Lv, Songtao Shou, Heng Jin

“…Additionally, the levels of IL-6 were detected by employing ELISA kits. RNA sequencing analysis was performed on cells treated with myoglobin and tocilizumab. …”
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ATP1B3 may promote glioma proliferation and migration through MAPK/NF-KB signaling pathway by Qikang Yan, Quan Sun, Yan Feng, Qingyun Hu, Jinling Zhu

“…Its connection to patient survival and prognosis; The siRNA interference approach instantly reduced the amount of ATP1B3 expression in the glioma cell lines U87MG and U251MG. …”
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Nutraceutical COMP-4 confers protection against endothelial dysfunction through the eNOS/iNOS-NO-cGMP pathway. by Monica G Ferrini, Andrea Abraham, Revecca Millán, Leslie Graciano, Sriram V Eleswarapu, Jacob Rajfer

“…NO intracellular content, nitrite formation and cGMP content in culture media, nitric oxide synthase (NOS) isoforms and mRNA content, pro-inflammatory cytokines, and PAI-1 expression and activity were measured. …”
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Evolution by natural selection is a scientific law and not just a theory by Daniel J.M. Crouch, Walter F. Bodmer

“…The developing knowledge of prokaryotic microorganisms and the discovery of DNA or RNA as the fundamental basis of inheritance in all living organisms, together with Mendel’s laws, now provide the four basic conditions for evolution by natural selection, namely reproduction that is stable, but allows for variation that can increase fitness in the broadest sense. …”
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Influence of Light Intensity on Tobacco Responses to Drought Stress by Brwa Rasool

“…A transcriptome profiling analysis of the tobacco responses to light intensity highlights the increased abundance of a large group of drought-related transcripts including DROUGHT-RESPONSIVE ELEMENT BINDING FACTORS (DREBs), C-REPEAT/DROUGHT-RESPONSIVE BINDING FACTOR 1 (CBF1), GLYCINE-RICH RNA BINDING PROTEINS (GRPs), WRKY33 and MYCs transcription factors, as well as zeaxanthin epoxidase, which play as a regulator of plant responses to water deficient condition. …”
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The Role of the Pathoanatomical Diagnosis «Chronic Gastritis» in the System of Personified Cancer Prevention by A. V. Kononov

“…Biopsy diagnosis of chronic gastritis combined with the evaluation of gene structure (genetic polymorphism) and epigenomic mechanisms (microRNA) allows the risk of gastric cancer to be ranked in a particular patient even with early precancerous changes in the gastric mucosa. …”
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Examining the epigenetic transmission of risk for chronic pain associated with paternal post-traumatic stress disorder: a focus on veteran populations by James Freeman, Sabrina Salberg, Melanie Noel, Richelle Mychasiuk

“…Numerous overlapping neurological pathways are implicated in both PTSD and chronic pain; many of which are susceptible to epigenetic modification, such as DNA methylation, histone modifications, and RNA regulation. Hence, epigenetic changes related to pain perception, inflammation, and neurotransmission may influence an individual’s predisposition to chronic pain conditions. …”
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Molecular anti-tumorigenic mechanism of Radix Polygoni Multiflori-derived exosome-like nanoparticles by Mingqing Yang, Lining Xu, Weiyu Wang

“…This indicated that RPM-ELNs could be internalized by hepatoma cells effectively and inhibit their proliferation and migration in vitro. RNA sequencing and qPCR experiments were performed, and the results of bioinformatic analyses revealed that RPM-ELNs could globally regulate expression levels of numerous genes associated with cancer and the cell cycle. …”
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Disruption of the β-catenin destruction complex via Ephexin1-Axin1 interaction promotes colorectal cancer proliferation by Jeeho Kim, Young Jin Jeon, In-Youb Chang, Jung-Hee Lee, Ho Jin You

“…Through comprehensive analysis, including RNA sequencing data from TCGA and functional assays, we observed that Ephexin1 promotes tumor proliferation and migration by activating the Wnt/β-catenin pathway. …”
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Expanding Viral Diversity in Rice Fields by Next-Generation Sequencing by Wang Haoran, Chen Guoqing, Feng Guozhong

“…Many novel viruses have been discovered in rice ecosystems through NGS technologies, with some also detected using metatranscriptomic and small RNA sequencing. These analyses greatly expand our understanding of viruses in rice fields and provide valuable insights for developing efficient strategies to manage insect pests and virus-mediated rice diseases.…”
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High-frequency PCR-testing as a powerful approach for SARS-CoV-2 surveillance in the field of critical infrastructure: A longitudinal, retrospective study in a German tertiary care... by Bastian Fischer, Martin Farr, Jan Gummert, Cornelius Knabbe, Tanja Vollmer

“…We therefore analyzed about 285.000 oral swab probes of 3.421 healthcare-workers concerning SARS-CoV-2 RNA positivity between November 2020 and February 2023. …”
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Martini compatible coarse-grained model of polyethylenimine for pulmonary gene delivery by Graham Lunt, Niloofar Hashemi, Subhamoy Mahajan, Tian Tang

“…Special attention is focused on parameterizing the non-bonded interactions associated with CG PEI, to ensure compatibility with Martini proteins, short interfering RNA, and phospholipids that are essential components in pulmonary gene delivery. …”
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Epigenetic regulation in female reproduction: the impact of m6A on maternal-fetal health by Peipei Li, Yumeng Lin, Hongyun Ma, Jiao Zhang, Qiaorui Zhang, Ruihua Yan, Yang Fan

“…Epigenetics plays an irreplaceable role in gynecologic diseases. As an important mRNA modification, m6A is involved in the maturation of ovum cells and maternal-fetal microenvironment. …”
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