Recent advances of nanovaccines on cancer theranostics by Xiaobing Yuan, Xiaobing Yuan, Xiaobing Yuan, Tianyou Xu, Mubashir Hussain, Bin Liu, Jianwei Zhu, Jianwei Zhu

“…We believe that polymer nanoparticle-based nanovaccines have the most widespread applications currently, while liposome nanovaccines using mRNA are expected to see greater development in the future. …”
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Sophocarpine inhibits the proliferation and induces apoptosis of glioblastoma cells through regulating the miR-21/PTEN/PI3K/AKT axis by Feng Si, Qian Wang, Fei Chen, Xiangdong Lu

“…CCK-8 was applied to analyze cell toxicity and proliferation. qRT-PCR and western blot were used to measure RNA and protein expressions, respectively. Cell cycle and cell apoptosis were determined by flow cytometry assay. …”
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Recent updates of centromere proteins in hepatocellular carcinoma: a review by Zhongyuan Yang, Wenjiao Chen, Yunhui Liu, Yuxin Niu

“…For example, small ribonucleic acid (RNA) can silence CENP expression and prevent the occurrence and development of liver cancer. …”
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Acid sphingomyelinase modulates anxiety-like behavior likely through toll-like receptor signaling pathway by Huiqi Yuan, Yanan Xu, Hailun Jiang, Meizhu Jiang, Luofei Zhang, Shifeng Wei, Cao Li, Zhigang Zhao

“…In the present study, we employed Asm-knockout (Asm KO) mice to investigate the association between Asm and anxiety using behavioral tests, RNA sequencing, q-PCR, immunohistochemical staining, and other methods. …”
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Resveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways by Saki Okamoto, Saya Kakimaru, Mayuko Koreishi, Mika Sakamoto, Yoshimasa Nakamura, Hideya Ando, Yoshio Tsujino, Ayano Satoh

“…At concentrations of 25–50 μM, resveratrol increased autophagy as measured by microtubule-associated protein 1A/1B-light chain 3 (LC3)-II/LC3-I ratio and enhanced lysosomal activity as assessed by a lysosomal activity reporter system. RNA sequencing revealed upregulation of lysosomal and autophagy-related genes, including cathepsins. …”
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Characterization and molecular biological identification of tick species in Nanjing city by Luyao SUN, Xuemin CHEN, Bingdong SONG, Yan ZHANG, Yanqun SUN, Yue MIN, Shougang ZHANG

“…The ticks were identified by morphological classification and molecular biological identification based on 16S rRNA gene. ResultsA total of 1 523 ticks were collected, including 993 Haemaphysalis longicornis (65.20% of all ticks collected), 529 Haemaphysalis flava (34.73%), and one Haemaphysalis phasiana (0.07%). …”
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PCR-based detection of Botryosphaeria canker pathogens in fig trees by Mahdiyeh Ghaedi, Zeinab Bolboli, Hamed Negahban, Reza Mostowfizadeh-Ghalamfarsa

“…In our study, we developed a PCR assay using three carefully selected primer pairs based on sequence differences in two protein-coding genes, β-tubulin and RNA polymerase II. The species-specific primers TUB-Bd1 (specific for Botryosphaeria dothidea), TUB-Np1 (specific for Neofusicoccum parvum), and RPB-Nd2 (specific for Neoscytalidium dimidiatum) effectively amplified target gene sequences in pure cultures and infected tissue samples using PCR and nested PCR conditions. …”
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A Nonsynonymous Single-nucleotide Polymorphism in SLC24A5 Regulates Feather Pigment Deposition in Chinese Yellow Quail by Xiaohui Zhang, Fanghu Wu, Yanxia Qi, Yuanyuan Shang, Lingyun Fan, Yifei Wang

“…Mutant individuals had significantly lower total melanin content in the feathers and tyrosinase activity in dorsal skin, in spite of no significant difference in SLC24A5 mRNA expression in the same tissues. This study indicates that the g.8884145A/G mutation reduced tyrosinase activity by affecting the function of the SLC24A5 protein, which in turn decreased melanin content of down feathers in Chinese yellow quail.…”
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LINC02282 promotes DNA methylation of TRIM6 by recruiting DNMTs to inhibit the progression of Parkinson's disease by Lu Han, Chuansheng Zhao, Feng Jin, Rongfeng Jiang, Hao Wu

“…The goal of the present study was to elucidate the mechanism by which LINC02282, a significantly downregulated lncRNA in the GEO database, elicits neuroprotective effects on PD. …”
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Stochastic processes govern gut bacterial community assembly in a Schistosoma mansoni-transmitting snail, Biomphalaria straminea. by Zhanhong Yuan, Jinni Hong, Jehangir Khan, Jinghuang Lu, Benjamin Sanogo, Zhongdao Wu, Xi Sun, Datao Lin

“…To better understand the balance between stochastic and deterministic processes in the snail gut microbial assembly and their associations with snail fitness, we used the freshwater snail Biomphalaria straminea as a model and analyzed the gut bacterial communities from 118 samples via high-throughput sequencing of the 16S rRNA gene.<h4>Results</h4>This study reveals that Proteobacteria and Bacteroidota dominate the gut microbiota of B. straminea. …”
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TDP-43 as a potential retinal biomarker for neurodegenerative diseases by Margit Glashutter, Margit Glashutter, Printha Wijesinghe, Joanne A. Matsubara, Joanne A. Matsubara

“…Animal model studies have been pivotal in exploring TDP-43’s roles in the retina, including its nuclear and cytoplasmic localization, RNA binding properties, and interactions with other proteins. …”
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Identification of deep intronic variants in junctional epidermolysis bullosa using genome sequencing and splicing assays by Fuying Chen, Ruoqu Wei, Yumeng Wang, Qiaoyu Cao, Jianbo Wang, Chenfei Wang, Dingjin Yao, Zhirong Yao, Cheng Ni, Ming Li

“…Our results emphasize the importance of selecting the right cell line for mRNA analysis.…”
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Endometrial polyps with bizarre stromal cells: a Benign or a low-grade lesion? by Musen Wang, Fei Gao, Hongkai Zhang, Wenxin Zheng

“…The histopathology mirrored the initial findings, showing no myometrial invasion, prompting a re-evaluation of the presumed benign nature of the polyp given its rapid growth. Remarkably, RNA sequencing analysis of the polyp detected a JAK2::NFIB gene fusion, a novel finding for endometrial polyps with atypical stromal cells, the clinical implications of which remain to be elucidated. …”
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Mettl3-m6A-NPY axis governing neuron–microglia interaction regulates sleep amount of mice by Qihang Sun, Jinpiao Zhu, Xingsen Zhao, Xiaoli Huang, Wenzheng Qu, Xia Tang, Daqing Ma, Qiang Shu, Xuekun Li

“…In the present study, we showed that the deficiency of RNA m6A methyltransferase Mettl3 in excitatory neurons specifically induces microglia activation, neuroinflammation and neuronal loss in thalamus of mice. …”
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Antimicrobial peptide AP2 ameliorates Salmonella Typhimurium infection by modulating gut microbiota by Lianglan Li, Qiufen Mo, Yi Wan, Yuanhao Zhou, Weiqin Li, Weifen Li

“…These effects were evidenced by reduced ST-induced body weight loss and lower levels of serum inflammatory cytokines. A 16 S rRNA-based analysis of the cecal microbiota revealed that AP2 significantly modulated the gut microbiota, increasing the relative abundance of Bifidobacterium while decreasing that of Akkermansia at the genus level. …”
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CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects by Vijay Sheri, Harikrishnan Mohan, Phanikanth Jogam, Anshu Alok, Gulab Khan Rohela, Baohong Zhang

“…To ensure specificity and avoiding off-target effects, we need to optimize various parameters such as target site, guide RNA design, and choosing right Cas variants. We outline the future prospects of CRISPR/Cas in cotton breeding, suggesting areas for further research and innovation. …”
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Studying the impact of chitosan salicylaldehyde/schiff base/CuFe2O4 in PC3 cells via theoretical studies and inhibition of PI3K/AKT/mTOR signalling by Ghada H. Elsayed, Asmaa M. Fahim

“…After 48 h, PC3 cell proliferation was significantly inhibited by chitosan salicylaldehyde/CuFe2O4 and chitosan salicylaldehyde (IC50 = 35.3 and 45.55 µg/ml, respectively) without any effects on normal HSF cells. The mRNA expression levels of PI3K, AKT, mTOR, and CCND1 were examined in PC3-treated cells by using QRT-PCR, and the results demonstrated that, by down-regulating the expression levels of these genes, chitosan salicylaldehyde/CuFe2O4 significantly affected prostate cancer cell proliferation, progression, and autophagy more than chitosan salicylaldehyde. …”
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Basic Molecular Biology, Metabolic and Immunological Mechanisms of Fecal Microbiota Transplantation by O. V. Goloshchapov, A. V. Beynarovich, A. B. Chukhlovin, R. V. Klementeva, A. A. Shcherbakov, E. A. Bakin, I. K. Golubovskaya, N. D. Ventslovayte, O. S. Yudintseva, M. V. Gorchakova, V. E. Karev, M. A. Kucher, M. A. Suvorova, E. V. Svetkina, G. G. Rodionov, V. V. Gostev, S. V. Sidorenko, I. S. Moiseev, A. D. Kulagin

“…To monitor the basic effects of FMT, we performed targeted DNA sequencing of 16S rRNA gene (V3–V4) using MiSeq platform as well as multiplex real-time PCR, MS/gas chromatography technique, immunophenotyping of blood lymphocytes, histological and immunohistochemical techniques.Clinical case. …”
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Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. by Sabiha S. Salih, Shno J. Mohammed, Imad M Noori, Lana MA Mohammed, Taib A. Hama Soor

“…Molecular identification of Proteus bacteria was confirmed by PCR amplification of part of 16S rRNA using Proteus specific set of primers.  70 meat samples (cattle, sheep, chicken, turkey, goat, and fish) were collected in local meat shops in the center of Sulaimani city. 29 (41.4%) samples were positive to Proteus species and 22 (75.87%) isolates were P. mirabilis and seven (24.13%) were P. vulgaris based on conventional biochemical tests. …”
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