CAR T-cell detection scoping review: an essential biomarker in critical need of standardization by Naomi Taylor, Nirali N Shah, David P Turicek, Victoria M Giordani, Josquin Moraly

“…While additional detection techniques–including droplet digital PCR, NanoString, and single-cell RNA sequencing–were reported in follow-up publications, inconsistencies with respect to detection time points and frequency remained, with a significant amount of quantitative data still not readily available. …”
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Engineered CAF-cancer cell hybrid membrane biomimetic dual-targeted integrated platform for multi-dimensional treatment of ovarian cancer by Yuwei Yao, Jiarui Zhang, Kexin Huang, Yingying Peng, Shuangshuang Cheng, Shuangge Liu, Ting Zhou, Jinhua Chen, Haojia Li, Yingchao Zhao, Hongbo Wang

“…Result We constructed PH20-overexpressing cancer-associated fibroblast (CAF)-cancer hybrid-cell membrane vesicles (PH20/CCM) for the dual-targeted delivery of carboplatin (CBP) and siRNA targeting p65 (sip65) loaded on the poly (dimethyl diallyl ammonium chloride) (PDDA)-modified MXene (PMXene), named PMXene@CBP-sip65 (PMCS). …”
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Advancing the Indian cattle pangenome: characterizing non-reference sequences in Bos indicus by Sarwar Azam, Abhisek Sahu, Naveen Kumar Pandey, Mahesh Neupane, Curtis P Van Tassell, Benjamin D Rosen, Ravi Kumar Gandham, Subha Narayan Rath, Subeer S Majumdar

“…Furthermore, 926 BICIs were identified within 682 protein-coding genes, 54 long non-coding RNAs (lncRNA), and 18 pseudogenes. These protein-coding genes were enriched for functions such as chemical synaptic transmission, cell junction organization, cell-cell adhesion, and cell morphogenesis. …”
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New insights into the mechanism of triphenyl phosphate and its metabolite diphenyl phosphate in diabetic kidney disease by Ting Fang, Qiaoyan Liu, Xinxin Huangfu, Hongkai Zhu, Hongwen Sun, Liming Chen

“…The study also detected mRNA expression of core targets molecularly docked with TPHP and DPHP using real-time-PCR. …”
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Single-cell atlas of human pancreatic islet and acinar endothelial cells in health and diabetes by Rebecca Craig-Schapiro, Ge Li, Kevin Chen, Jesus M. Gomez-Salinero, Ryan Nachman, Aleksandra Kopacz, Ryan Schreiner, Xiaojuan Chen, Qiao Zhou, Shahin Rafii, David Redmond

“…Here, we develop strategies to enrich islet-specific endothelial cells (ISECs) and acinar-specific endothelial cells (ASECs) from three human pancreases and corroborate these findings with three published pancreatic datasets. Single-cell RNA sequencing reveals the unique molecular signatures of ISECs, including structural genes COL13A1, ESM1, PLVAP, UNC5B, and LAMA4, angiocrine genes KDR, THBS1, BMPs and CXCR4, and metabolic genes ACE, PASK and F2RL3. …”
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USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer by Zhongdong Xie, Hanbin Lin, Yuecheng Wu, Yanan Yu, Xintong Liu, Yating Zheng, Xiaojie Wang, Jiashu Wu, Meifang Xu, Yuting Han, Qiongying Zhang, Yu Deng, Lin Lin, Yan Linzhu, Li Qingyun, Xinjian Lin, Ying Huang, Pan Chi

“…Examination of clinical samples confirmed that USP4 expression positively correlates with CENPF protein expression, but not mRNA transcript levels. Taken together, this study describes a novel USP4-CENPF signaling axis which is crucial for CRC metastasis, potentially serving as a therapeutic target and a promising prognostic biomarker for CRC.…”
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Synergistic strategies for glioblastoma treatment: CRISPR-based multigene editing combined with immune checkpoint blockade by Xiaolin Liu, Xiao Liu, Xiaonan Luo, Maorong Zhu, Nannan Liu, Juan Li, Qi Zhang, Cheng Zou, Yuxin Wu, Zhengcong Cao, Shuangxin Ma, Weizhong Wang, Guangzhao Yang, Jintao Gu, Wei Liu, Meng Li, Anan Yin, Yalong He, Wei Lin

“…Furthermore, the EVs contained Cas9 protein and sgRNA for precise and minimally invasive gene therapy, which addressing the key barriers associated with in vivo CRISPR‒Cas9 gene editing treatment. …”
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Suppressing GDF15 enhances the chemotherapeutic effect of 5 FU on MSI-H CRC by regulating the ferroptosis pathway SLC7A11/GSH/GPX4 by Zhi min Huang Fu, Ming Xiao, Hailun Xie, Shuxian Zhang, Tang Yi, Qingshu Li, Ming Li, Yalan Wang

“…Suppressing GDF15 by siRNA resulted in a substantial decrease in cell viability and proliferation. …”
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Transcriptional regulatory network analysis identifies GRN as a key regulator bridging chemotherapy and immunotherapy response in small cell lung cancer by Seungyeul Yoo, Ayushi S. Patel, Sarah Karam, Yi Zhong, Li Wang, Feng Jiang, Ranran Kong, Sharon Bikvan, Wenhui Wang, Abhilasha Sinha, Charles A. Powell, Jun Zhu, Hideo Watanabe

“…GRN and its associated genes were linked to cancer cell intrinsic immunogenicity, and single-cell RNA-seq data revealed that GRN expression is particularly high in subsets of tumor-associated macrophages. …”
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Molecular characterization of putative antibiotic resistance determinant and virulence factors genes of Acinetobacter baumannii strains isolated from intensive care unit patients i... by Faris A. Alanazi, Khalid S. Almaary, Turki M. Dawoud, Mohamed A. El-Tayeb, Yahya B. Elbadawi, Ayman S. Mubarak, Ali M. Somily

“…Method: The A. baumannii isolates were confirmed by the VITEK-2 automated system and 16S rRNA phylogenetic relatedness. The phenotypic and genotypic resistance patterns of these isolates were also analyzed using conventional and molecular methods. …”
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Ficus deltoidea standardized methanolic extract improves kidney structure and function against streptozotocin-induced diabetic nephropathy in rats by Nurdiana Samsulrizal, Noor Syaffinaz Noor Mohamad Zin, Nur Syimal`ain Azmi, Mohd Daud Bakar, Hafandi Ahmad, Yong-Meng Goh

“…We noticed that biochemical and pathological changes corresponded to the infrared (IR) peaks at 1545 cm-1 and 1511 cm-1 (amide II), 1246 cm-1 (DNA/RNA phosphate backbones), and 1181 cm-1 (C–O vibrations). …”
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PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic by Haomiao Li, Xuri Xie, Tianming Qiu, Jingyuan Zhang, Jie Bai, Guang Yang, Ningning Wang, Xiaofeng Yao, Xiance Sun

“…Remarkably, arsenic exposure increased the expression levels of Perilipin 5 (PLIN5), a lipid droplet-associated protein, both at the mRNA and protein levels. Moreover, silencing PLIN5 influenced arsenic-induced lipolysis. …”
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Adjunctive diagnostic tool for histopathological classification of congenital mesoblastic nephroma based in molecular genetic findings by Hiroshi Hamada, Kenichi Kohashi, Takeshi Iwasaki, Mikiko Hashisako, Yuko Hino, Masahiro Fukuhara, Amane Kamouchi, Naonori Kawakubo, Tatsuro Tajiri, Yoshinao Oda

“…Reverse transcription-polymerase chain reaction was performed for the NTRK gene fusion and EGFR-ITDs in all cases. Comprehensive mRNA analysis was performed using the nCounter® Gene Expression Assay. …”
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Genetically edited human placental organoids cast new light on the role of ACE2 by Anya L. Arthurs, Bianca Dietrich, Martin Knöfler, Caleb J. Lushington, Paul Q. Thomas, Fatwa Adikusuma, Jessica M. Williamson, Susan Babikha, Tyla Damhuis, Tanja Jankovic-Karasoulos, Melanie D. Smith, Kirsty G. Pringle, Claire T. Roberts

“…Placental organoids with the ACE2 rs2074192 TT genotype had significantly higher expression of ACE2 mRNA and ACE2 protein with elevated ACE2:ACE expression ratio and no change in ACE protein. …”
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Exploring the influence of pre-analytical variables on gene expression measurements and relative expression orderings in cancer research by Tian Tian, Guie Lai, Ming He, Xiaofang Liu, Yun Luo, You Guo, Guini Hong, Hongdong Li, Kai Song, Hao Cai

“…These paired samples were utilized to assess the impact of pre-analytical variables on gene expression measurements and REOs, including sampling methods, tumor sample heterogeneity, fixed time delays, preservation conditions, degradation levels, library preparation kits, amplification kits, RNA quantity, measuring platforms, and laboratory sites at single and multi-variable level. …”
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Is The SARS-CoV2 Evolved in Human Being: A prospective Genetic Analysis by Salvatore Dimonte, Paywast Jamal Jalal, Hiwa Abdulrahman Ahmad, Safa Bakr Karim, Taib Ahmed Hama Soor, Salar Ibrahim Ali, Muhammed Babakir-Mina, Francesco Greco

“…The evolutionary changes observed in the inserted 23 amino acids in the RNA binding domain (RBD) of the coronvirus spike glycoprotein which cannot be detected in any other human coronavirus. …”
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Bioinformatics insights into mitochondrial and immune gene regulation in Alzheimer's disease by Tian Meng, Yazhou Zhang, Yuan Ye, Hui Li, Yongsheng He

“…Moreover, qRT-PCR validation confirmed that the mRNA expression of the five biomarkers was consistent with bioinformatics results. …”
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Occurrence of macrolides resistance in Legionella pneumophila ST188: Results of the Belgian epidemiology and resistome investigation of clinical isolates by Charlotte Michel, Fedoua Echahidi, Geraldine De Muylder, Max Sewell, Ian Boostrom, Olivier Denis, Owen B. Spiller, Denis Pierard

“…Genotypic screening identified a 23S ribosomal RNA mutation linked to a high-level macrolide resistance in one isolate of ST188, which is genetically closed to resistant isolates from France. …”
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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Also, VWF and ITGB2 were significantly related to the clinical features, including age, serum creatinine level, and GFR (MDRD). Besides, mRNA and protein expression levels of ITGB2 in HG-treated HK-2 cells were remarkably elevated. …”
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Mural cell dysfunction contributes to diastolic heart failure by promoting endothelial dysfunction and vessel remodelling by Mandy O. J. Grootaert, Alessandra Pasut, Jana Raman, Steven J. Simmonds, Bram Callewaert, Ümare Col, Mieke Dewerchin, Peter Carmeliet, Stephane Heymans, Elizabeth A. V. Jones

“…We combined single-cell RNA sequencing, NichetNet analysis and histology to determine the role of mural cell dysfunction in HFpEF. …”
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