Case study of a neuroendocrine tumor of uncertain origin: single-cell transcriptomics unravels potential primary location by Frolova Tatyana, Ekaterina Avsievich, Diana Salimgereeva, Zoia Antysheva, Alesia Maluchenko, Denis Maksimov, Ilia Feidorov, Mark Voloshin, Olga Glazova, Natalia Bodunova, Nikolay Karnaukhov, Pavel Volchkov, Julia Krupinova

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Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City by Fahima Akter, Md. Shamimuzzaman, Susmita Roy Chowdhury, Faria Islam, Rajib Kanti Roy

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Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City by Fahima Akter, Md. Shamimuzzaman, Susmita Roy Chowdhury, Faria Islam, Rajib Kanti Roy

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Engineering of SauriCas9 with enhanced specificity by Xiaoqi Zhang, Chen Tao, Miaomiao Li, Sufang Zhang, Puping Liang, Yan Huang, Huihui Liu, Yongming Wang

Subjects: “…MT: RNA/DNA Editing…”
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Structural insights into dynamics of the BMV TLS aminoacylation by Wen Yang, Ran Yi, Jing Yao, Yongxiang Gao, Shanshan Li, Qingguo Gong, Kaiming Zhang

“…Abstract Brome Mosaic Virus (BMV) utilizes a tRNA-like structure (TLS) within its 3’ untranslated region to mimic host tRNA functions, aiding aminoacylation and viral replication. …”
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Non-coding RNAs and exosomal ncRNAs in colorectal cancer by Yalda Zhoulideh

“…In recent years, increasing attention has been paid to the role of non-coding RNA (ncRNA) and exogenous ncRNA in the development and progression of CRC. ncRNAs play an essential role in the regulation of gene expression and cell function, and dysregulation of ncRNA expression is associated with the onset and progression of CRC. …”
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Lack of Tissue Virological Response as a Predictor of Relapse in Chronic Hepatitis D after Completion Bulevirtide Therapy by P. O. Bogomolov, N. A. Shub, N. A. Gasilova, A. A. Belyakova, T. G. Lidzhieva, E. A. Kondrasheva, N. A. Barsukova, M. V. Arapova, E. A. Isaeva, S. V. Koblov, M. V. Kalashnikov, O. S. Kuzymina, A. Yu. Demyanov, A. O. Bueverov, I. V. Maev

“…Aim: to evaluate the significance of a positive polymerase chain reaction result for hepatitis D virus RNA (HDV RNA) in liver biopsy specimens of patients with chronic hepatitis D (CHD) after completion of antiviral therapy (AVT) as a predictor of infection relapse.Materials and methods. …”
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Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress by Ping Han, Jianming Chen, Zhennan Sun, Shengjie Ren, Xubo Wang

“…Results In this study, we evaluated the suitability of eight genes including ribosomal protein L6 (rpl6), ribosomal protein L9 (rpl9), delta (4)-desaturase, sphingolipid 1 (degs1), cathepsin L (ctsl), eukaryotic translation elongation factor 1 gamma (eef1g), NSA2 ribosome biogenesis homolog (nsa2), eukaryotic translation initiation factor 3, subunit E, a (eif3ea), glutamine amidotransferase class 1 domain containing 1 (gatd1) analyzed from RNA sequencing (RNA-Seq) data and two genes including β-actin (actb) and 18S rRNA ribosomal RNA (18S RNA) selected from literature to obtain the best internal controls in qRT-PCR analysis of Japanese flounder under temperature stress. …”
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Comparison of saliva and blood derived cell free RNAs for detecting oral squamous cell carcinoma by Yuan Hu, Manbin Xu, Muyuan Liu, Hanwei Peng

“…In our study to enhance OSCC detection, we analyzed cell-free RNA (cfRNA) transcriptomes (mRNA, lncRNA) from blood (plasma) and saliva samples of individuals in three groups: OSCC group, oral benign tumor group, and a normal control group. …”
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3-Acetyldeoxynivalenol induces pyroptosis in leydig cells via METTL3-mediated N6-methyladenosine modification of NLRP3 by Yangyun Wang, Chaoliang Shi, Wei Jiao, Xiaoxiang Wan

“…RNA immunoprecipitation (RIP) assays further demonstrated that IGF2BP1 selectively recognizes m6A-modified NLRP3 mRNA, contributing to its stabilization. …”
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Oncometabolite D-2HG drives tumor metastasis and protumoral macrophage polarization by targeting FTO/m6A/ANGPTL4/integrin axis in triple-negative breast cancer by Siyue Zhang, Ning Zhang, Tong Wan, Yinqiao He, Jie Hao, Yiwei Liu, Yidong Liu, Bing Chen, Wenjing Zhao, Lijuan Wang, Dan Luo, Chao Gao, Qifeng Yang

“…RNA pull-down assays were further applied to identify the reader that could specifically recognize the m6A modification on angiopoietin like 4 (ANGPTL4) mRNA and RNA immunoprecipitation was used to confirm the findings. …”
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Transient ocular hypertension remodels astrocytes through S100B. by Weiran Huang, Kenji Matsushita, Rumi Kawashima, Susumu Hara, Yuichi Yasukura, Kaito Yamaguchi, Shinichi Usui, Koichi Baba, Andrew J Quantock, Kohji Nishida

“…Glial fibrillary acidic protein (GFAP), S100B, and actin staining were used to characterize astrocyte morphology and cytoskeleton, with qPCR used to measure mRNA expression. We also silenced S100B expression and conduct RNA sequencing on ONH astrocytes. …”
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Exploring the prognostic role of microbial and genetic markers in lung squamous cell carcinoma by Fan Yang, Xiaodong Jia, Zihuan Ma, Siyao Liu, Chunzi Liu, Dan Chen, Xiuju Wang, Niansong Qian, Hui Ma

“…LUSC patient data from The Cancer Genome Atlas (TCGA), including microbial genus level abundance data and RNA sequencing (RNA-Seq) data, were used as a training dataset. …”
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Importance of the 5’ untranslated region for recombinant enzyme production in isolated Bacillus subtilis 007 by Jana Senger, Adriana Schulz, Ines Seitl, Martin Heider, Lutz Fischer

“…Since the aprE 5’ untranslated region contributes to a high mRNA stability, it was incorporated into the P43 construct to determine whether mRNA stability is responsible for the differences observed in β-galactosidase production. …”
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Integration of transcriptomics and metabolomics data revealed role of insulin resistant SNW1 gene in the pathophysiology of gestational diabetes by Ravi Bhushan, Rimjhim Trivedi, Ritu Raj, Anjali Rani, Sangeeta Rai, Anima Tripathi, Shiv Govind Rawat, Ajay Kumar, Dinesh Kumar, Pawan K. Dubey

“…The lack of complete understanding of its pathophysiological mechanisms and novel regulatory biomarkers makes early diagnosis difficult. High-throughput RNA sequencing and computational bioinformatics analyses were conducted to identify novel hub genes, and their regulatory mechanisms were validated through qRT-PCR, western blot, and siRNA-mediated knockdown studies. …”
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Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma by Wei-Ting Kuo, Yi-Chen Lee, Jia-Bin Liao, Ching-Jiunn Tseng, Yi-Fang Yang

“…Knockdown of SUMF2 significantly reduced the migration and invasion abilities of 5637 cells. Furthermore, SUMF2 mRNA levels negatively correlated with FBXW7 mRNA levels in BLCA. …”
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Inactivation of SARS-CoV-2 virus in saliva using a guanidium based transport medium suitable for RT-PCR diagnostic assays. by Sukalyani Banik, Kaheerman Saibire, Shraddha Suryavanshi, Glenn Johns, Soumitesh Chakravorty, Robert Kwiatkowski, David Alland, Padmapriya P Banada

“…A 1:2 (saliva:eNAT) dilution abrogated both CPE and detectable viral RNA after as little as 5 min incubation in eNAT. …”
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Role of exercise on ncRNAs and exosomal ncRNAs in preventing neurodegenerative diseases: a narrative review by Shangwu Liu, Runhong Zhang, Jamal Hallajzadeh

“…It is hypothesized that exercise-induced changes in ncRNA expression may regulate key processes involved in neuroprotection, including neuroinflammation, oxidative stress, protein aggregation, and synaptic function. …”
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Nitidine chloride inhibits the progression of hepatocellular carcinoma by suppressing IGF2BP3 and modulates metabolic pathways in an m6A-dependent manner by Dan-dan Xiong, Zhen-dong Chen, Jian-di Li, Yu-long Deng, Rong-quan He, Zhi-guang Huang, San-qi An, Yi-wu Dang, Gang Chen

“…Subsequently, employing RNA sequencing, RT-qPCR, WB, molecular docking, MD simulation, SPR, and CCK8 off-target assays, we pinpointed IGF2BP3 as a direct target of NC. …”
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FDFT1 inhibits macrophage M1 polarization and promotes colorectal cancer progression by GAO Yuan, HUANG Yulan, ZHAO Kun

“…Methods‍　‍Mouse colorectal cancer MC38 cells were divided into control group (si-NC) and experimental groups (the expression of enzymes in cholesterol synthesis pathway was interfered with siRNA for corresponding targets). RT-qPCR was used to detect the mRNA levels of corresponding targets in MC38 cells after transfection. …”
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