Host 3' flap endonuclease Mus81 plays a critical role in trimming the terminal redundancy of hepatitis B virus relaxed circular DNA during covalently closed circular DNA formation. by Hu Zhang, Quanxin Long, Yuanjie Liu, Alexander L Marchetti, Cheng-Der Liu, Ning Sun, Haitao Guo

“…Hepatitis B virus (HBV) relaxed circular DNA (rcDNA) possesses an 8-9 nucleotide-long terminal redundancy (TR, or r) on the negative (-) strand DNA derived from the reverse transcription of viral pregenomic RNA (pgRNA). It remains unclear whether the TR forms a 5' or 3' flap structure on HBV rcDNA and which TR copy is removed during covalently closed circular DNA (cccDNA) formation. …”
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YTHDF2 mediates the protective effects of MG53 on myocardial infarction injury via recognizing the m6A modification of MG53 by Zhaojie Li, Kai Li, Jianqiang Zhao

“…Furthermore, N6-methyl-adenosine (m6A) methylation is a mode of RNA modification that influences mRNA functions. …”
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A review of COVID-19 vaccination and the reported cardiac manifestations by Jamie Sin Ying Ho, Ching-Hui Sia, Jinghao Nicholas Ngiam, Poay Huan Loh, Nicholas Wen Sheng Chew, William Kok-Fai Kong, Kian-Keong Poh

“…In Singapore, 9.03 million doses of the mRNA COVID-19 vaccines by Pfizer-BioNTech and Moderna have been administered, and 4.46 million people are fully vaccinated. …”
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RETRACTED ARTICLE: Combined treatment of glibenclamide and CoCl2 decreases MMP9 expression and inhibits growth in highly metastatic breast cancer by Zhe Rong, Li Li, Fei Fei, Lailong Luo, Yang Qu

“…Some fresh tissues without necrosis were stored at −80°C for mRNA detection and the other tumor tissue was fixed with 10% formalin for H&E and immunohistochemical staining. …”
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MiR-25-3p regulates pulmonary arteriovenous malformation after Glenn procedure in patients with univentricular heart via the PHLPP2-HIF-1α axis by Junpei Kawamura, Munekazu Yamakuchi, Kentaro Ueno, Teruto Hashiguchi, Yasuhiro Okamoto

“…Microarray in situ hybridization was performed to assess the miRNA (miRNA) profiles of serum from pediatric patients (0–6 years of age) with G-PAVMs and after the Fontan procedure without G-PAVMs. …”
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CircWDR78 inhibits the development of colorectal cancer by regulating the miR-653-3p/RGS4 axis by Chu Hao, Yunju Pu, Jiunian Li, Zhi Zhong, Zhaohui Huang, Xue Wang

“…Methods Actinomycin D experiment and RNA digestion experiment were used to verify the circular structure characteristics of circWDR78. …”
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Omicron Positivity in Air of Hospital Settings Gathered COVID-19 Patients, Vaccinated/Unvaccinated Populations by Zeynep Ece Kuloğlu, Zeynep Bengi Eren, Bedirhan Haykar, Cansel Vatansever, Tayfun Barlas, Mert Ahmet Kuskucu, Gülen Güney-Esken, Füsun Can

“…The viral RNA was positive in 50% of the COVID-19 patient rooms, 33% of the febrile disease outpatient clinic, and 33% of the clinical laboratory waiting room. …”
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Gene function revealed at the moment of stochastic gene silencing by Shreyan Gupta, James J. Cai

“…Abstract Gene expression is a dynamic and stochastic process characterized by transcriptional bursting followed by periods of silence. Single-cell RNA sequencing (scRNA-seq) is a powerful tool to measure transcriptional bursting and silencing at the individual cell level. …”
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Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids by Lu Zhu, Wenhao Yang, Jiaxin Luo, Danli Lu, Yanan Hu, Rui Zhang, Yan Li, Li Qiu, Zelian Chen, Lina Chen, Hanmin Liu

“…Whole exome sequencing revealed that over 99% of single nucleotides were shared between the NO and matched BO and there was a 95% overlap in their RNA transcriptomes. RNA sequencing analysis of differentially expressed genes indicated a significant reduction in the immune response in NO. …”
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Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

“…Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons to model aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations. …”
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Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting by Rachel D. Altshuler, Megan A. M. Burke, Kristine T. Garcia, Kenneth Class, Raffaello Cimbro, Xuan Li, Xuan Li

“…Finally, we examined gene expressions in DS homogenates, to demonstrate cell-type specificity of gene alterations observed on abstinence day 28.ResultsOn abstinence day 1, we found mRNA expression of Gabrb3 decreased only in CTb-positive (but not CTb-negative) neurons of Meth rats compared with saline rats, while mRNA expression of Usp7 decreased in all sorted DS neurons. …”
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Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis by Anqi Wang, Sitong Yan, Weijia Jiang, Xiang Chen, Yuhan Huang, Xiangyu Zu, Xiao Du, Lulu Fan, Jiatao Liu, Guoping Sun

“…Public databases and miRNA-seq data were used to explore the TF-miRNA-CLIP4 regulatory network. …”
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Effects of prolonged NaHCO3 exposure on the serum immune function, antioxidant capacity, intestinal tight junctions, microbiota, mitochondria, and autophagy in crucian carp (Carass... by Jia Liu, Faye Zhang, Zihao Yan, Zhengyao Guo, Yuqian Lu, Baolan Yao, Yuehong Li, Wenfa Lv

“…The levels of ATG5, ATG7, and Beclin1 mRNA and protein were significantly increased along with the levels of LC3b mRNA and the ratio of protein LC3 II /LC3 I. …”
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A novel chitosan microsphere as food processing enzyme immobilization carrier and its application in nucleotide production by Xiao-Yan Yin, Rui-Fan Yang, Zhong-Hua Yang

“…Moreover, in PBR, the RNA hydrolysis conversion rate maintained 81 % after 24 h of continuous operation. …”
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Targeted inhibition of the PTEN/PI3K/AKT pathway by YSV induces cell cycle arrest and apoptosis in oral squamous cell carcinoma by Lin Qiu, Qian Gao, Yiheng Liao, Xinxin Li, Cuiying Li

“…Methods A series of in vitro experiments were conducted to assess the impact of YSV on OSCC cell viability, colony formation, cell cycle, and apoptosis. RNA sequencing (RNA-seq), molecular docking, and western blotting were employed to investigate the molecular mechanisms. …”
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Advances in Focal Segmental Glomerulosclerosis Treatment From the Perspective of the Newest Mechanisms of Podocyte Injury by Zhu Y, Xu G

“…Notably, some medications such as Rituximab and Sparsentan, may simultaneously target multiple downstream mechanisms, Furthermore, exploring molecular strategies for established medications and developing novel treatments guided by biomarkers such as Anti-CD40 antibody, blood microRNA, urinary microRNA, and tumor necrosis factor-alpha (TNF-α) may provide additional therapeutic avenues for patients with FSGS.Keywords: focal segmental glomerulosclerosis, podocyte injury, targeted therapies, biomarkers…”
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The emerging roles of aberrant alternative splicing in glioma by Reda Ben Mrid, Sara El Guendouzi, Marco Mineo, Rachid El Fatimy

“…Dysregulation of this process, through variations in spliceosome components, aberrant splicing factors and RNA-binding protein activity, disproportionate regulation of non-coding RNAs, and abnormal mRNA methylation, can contribute to the disruption of AS. …”
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Comprehensive review of preclinical evaluation strategies for COVID-19 vaccine candidates: assessing immunogenicity, toxicology, and safety profiles by Delaram Doroud, Mojtaba Daneshi, Fatemeh Kazemi-Lomedash, Zohre Eftekhari

“…Evaluating their biodistribution is crucial for newly developed vaccines, such as lipid nanoparticles –messenger RNA (LNP-mRNA), DNA, and Viral vectors in non-replicated (VVnr), or recombinant virus vaccines. …”
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Modulatory Impacts of Probiotic on the Synthesis of Claudins and Mucins in the Gastrointestinal Tract of Broiler Chicks by ESI Mohammed, M Al-Rasheed, MS Ahmed

“…ABSTRACT This study investigated the effect of 3 strains of probiotic bacteria on mRNA synthesis of some claudins (claudins1(CLD1), CLD3, and CLD5), and mucins (MUC5AC and MUC2) in broilers’ digestive systems. …”
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Blue light promotes conjunctival epithelial-mesenchymal transition and collagen deposition through ITGB4 by Qianjie Yang, Kuangqi Chen, Siyi Chen, Yinhao Wang, Yutong Xia, Jinbo Chen, Ye Shen

“…BL disrupted cell conjunction and regulated EMT-related proteins. RNA sequencing analysis revealed upregulation of ITGB4 and enrichment of cell migration and adhesion pathways. …”
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