Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications by Yurong Chen, Haibo Zhu, Fucheng Guo, Luyao Wang, Wenli Zhang, Ruizhi Liu, Xiaoling Zhang, Xiangpeng Dai

“…The warmed embryos which were frozen at 8-cell stage were cultured in vitro until the blastocyst stage and were transferred into recipients. …”
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Profiling the response of individual gut microbes to free fatty acids (FFAs) found in human milk by Megan E. Waller, Alyssa Gutierrez, Taylor D. Ticer, Janiece S. Glover, John E. Baatz, Carol L. Wagner, Melinda A. Engevik, Katherine E. Chetta

“…Select species of commensal and pathobiont genera (Bifidobacterium, Lactobacillus, Streptococcus, Staphylococcus, Enterococcus, Acinetobacter, Pseudomonas, Escherichia, and Klebsiella) were cultured with FFAs. The growth of all commensals, except for L. johnsonii, was significantly inhibited by the highest concentration (1 %) of all FFAs. …”
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Promoting ER stress in a plasmacytoid dendritic cell line drives fibroblast activation by Beatriz H. Ferreira, Inês S. Silva, Andreia Mendes, Fátima Leite-Pinheiro, Adrienne W. Paton, James C. Paton, Iola F. Duarte, Philippe Pierre, Catarina R. Almeida

“…More specifically, IMR-90 fibroblasts were co-cultured with CAL-1, a pDC cell line. ER stress was then induced by the bacterial toxin SubAB. …”
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Optimizing winged-bean meal through oven and autoclave heating as viable alternative for plant-based aquafeed by Shobrina Silmi Qori Tartila, Tholibah Mujtahidah, Muh. Azril, Anjali Apta Pramudita, Lusi Septiani, Bagas Putra Satria, Ignatius Abel Cristanto, Muhammad Abizar Sakti Oktavian, Sultan Jibran Risqulloh, Santika Dewi Astuti

“…Data were also compared to the nutrient requirements of several cultured freshwater fish in Indonesia. Heating treatments improved in vitro trypsin activity (1300 units/O and 1135 units/A vs. 835 units) but reduced protein (29.05±1.96%/A and 31.73±1.52%/O vs. 35.17±0.38%) and moisture contents. …”
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Comparison evaluation of bacterial DNA extraction methods for improved molecular diagnostic accuracy of sepsis-causing pathogens in clinical whole blood samples by Byungjoon Na, Junghun Park, Sojin Park, Eunseon Park, Jimin Jang, Yu-Hee Kim, Jinyeop Lee, Hae-Sun Chung

“…Current diagnostic methods such as blood cultures are time-consuming, whereas molecular diagnostic techniques represent a promising alternative for faster pathogen detection. …”
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The philosophical foundations of digital twinning by David J Wagg, Christopher Burr, Jason Shepherd, Zack Xuereb Conti, Mark Enzer, Steven Niederer

“…As a result, members of the newly forming digital twin community are often talking at cross-purposes, based on different starting points, assumptions, and cultural practices. These differences are due to the philosophical world-view adopted within specific communities. …”
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Muscarinic cannabinoid suppression of excitation, a novel form of coincidence detection by Michaela Dvorakova, Ken Mackie, Alex Straiker

“…These receptors are implicated in several forms of synaptic plasticity – depolarization-induced suppression of excitation (DSE), metabotropic suppression of excitation (MSE), long term depression (LTD) and activation-dependent desensitization. Cultured autaptic hippocampal neurons express all of these, illustrating the rich functional and temporal heterogeneity of CB1 at a single set of synapses. …”
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Re-conceptualizing trafficking-in-persons victimization using latent class analysis: Results from a community study in Cape Town, South Africa by Annah K. Bender, Erica L. Koegler, Edna G. Rich, Nicolette V. Roman, Rumi Kato Price

“…The difficulty of reaching a clandestine population is complicated by the lack of standardized definitions and culturally responsive assessments to identify victims and route them to appropriate care. …”
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Molybdate delays sulphide formation in the sediment and transfer to the bulk liquid in a model shrimp pond by Torun, Funda, Hostins, Barbara, De Schryver, Peter, Boon, Nico, De Vrieze, Jo

“…Shrimp are commonly cultured in earthen aquaculture ponds where organic-rich uneaten feed and faeces accumulate on and in the sediment to form anaerobic zones. …”
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Finger roots (Uvaria chemea) and African greenheart (Cylicodiscus gabunensis): Alternative potent therapy to sildenafil in erectile dysfunction by Etukudo Okon Jimmy, Grace Edet Bassey, Abijah Ekwere, Udoh Idorenyin Umoh, Adeyemi Fatai Odetayo, Udoh Nsikan Malachy

“…Traditional herbal remedies, rooted in cultural practices, have been explored as alternative therapies for ED, and this study focuses on Uvara chemea and Cylicodiscus gabunensis, two plants with reported medicinal properties, as potential alternatives to sildenafil, a standard pharmaceutical for ED. …”
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Coexistence of blaMIR-1 and blaNDM-1 resistance genes with a novel ST type in Enterobacter roggenkampii from a stool sample: A genome sequencing study by Qin Wang, Sayyed Salman, Ye Luo, XiaoMei Tong

“…Methods: A faecal sample was diluted in Luria-Bertani broth and incubated overnight. Cultures were then spread on MacConkey agar containing meropenem and incubated for 18–24 h to select carbapenem-resistant Enterobacteriaceae. …”
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Integrated high-precision real scene 3D modeling of karst cave landscape based on laser scanning and photogrammetry by Congyuan Zhang, Jianping Chen, Ping Li, Sipeng Han, Jie Xu

“…Abstract In recent years, the application of real scene 3D technology has become widespread in urban planning and cultural heritage protection. However, there has been relatively little attention paid to the construction of real scene 3D models for special natural landscapes such as caves. …”
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Professional experiences on use of the mental health act in ethnically diverse populations: a photovoice study by Roisin Mooney, Paul McCrone, Rose McCabe, Kamaldeep Bhui, Brian Brown, Colin King, Andrew Molodynski, Nusrat Husain, Jacqueline Sin, Karen Newbigging, Michael Larkin, Neil Caton, Raghu Raghavan, Doreen Joseph, Anthony Zigmond, Michelle Yeung, Julie Carr, Phuong Hua, Frank Keating, Tara Morey, Claire Dempsey, Rahul Battacharya, Nuwan Dissanyake, Rochelle Ramkisson, Laura Dixon, Kopal Tandon, Gary Roberts, Paul Illingworth, Jo White, Karen Persaud, Sayeeda Basharat, Tripti Kandia, Sandra Griffiths, Michael Molete

“…We undertook a thematic analysis.Results Ineffective communications between inpatient and community services, insufficient staff capacity, a lack of continuity of care and language and cultural constraints meant MHA assessments were lacking information, leading to elevated perceptions of risk. …”
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AxiWorm: a new tool using YOLOv5 to test antiparasitic drugs against Trichinella spiralis by Javier Sánchez-Montejo, Miguel Marín, María Alejandra Villamizar-Monsalve, María del Carmen Vieira, Belén Vicente, Rafael Peláez, Julio López-Abán, Antonio Muro

“…Material and methods Morphological properties were used to distinguish L1 from debris after pepsin muscle digestion and distinguish healthy (serpentine) or damaged (coiled) L1s after 72 h untreated or treated with albendazole or mebendazole cultures. An AxiDraw robotic arm with a smartphone was used to scan 96 well microplates and store photographs. …”
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The Cytotoxicity Assessment of Grapefruit Peel Essential Oil and Its Effect on Frying Stability of Sunflower Oil by Ghania Kaanin-Boudraa, Fatiha Brahmi, Linda Grimaud, Mohamed Lamine Freidja, Ourdia-Nouara Kernou, Daniel Henrion, Samir Hadjal, Khodir Madani, Lila Boulekbache-Makhlouf

“…Various EO concentrations (0.01-0.5 mg/ml) were added to cultured cells Human Umbilical Vein Endothelial Cells (HUVECs) and Human Stellar Hepatic Cell lines LX-2 (SCC064) and incubated for 24 and 48 h. …”
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Gender disparity in living organ donation: a qualitative analysis of experiences and perceptions of female donors in Pakistan by Muhammad Ishaq, Javeria Saleem, Ruhma Binte Shahzad, Mahrukh Iqbal, Abida Tehreem, Raja Sajjad Asghar, Aleena Touqeer, Gul Mehar Javaid Bukkhari

“…Objectives This study aims to explore and analyse the interplay of sociocultural, psychological and institutional factors, gender roles and power dynamics in shaping the decision-making processes and experiences of living kidney and liver donors in Punjab, specifically focusing on women.Design This study employed a qualitative approach, using in-depth interviews to delve into the narratives and experiences of living kidney and liver donors in Punjab.Setting Punjab Human Organ Transplantation Authority (PHOTA), Lahore, Punjab, Pakistan.Participants 17 participants registered at the PHOTA for kidney or liver donation.Results Interviews with living donors at PHOTA, Lahore, provided qualitative data, which was then systematically coded and analysed to identify recurring themes that explore the underlying social and cultural reasons for the observed gender disparity. …”
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Building Sustainability Strategies in The Creative Culinary Industry Sector To Enhance Tourist Destination Attraction in Semarang Regency by Nurchayati, Bambang Riyadi, Lisa Efri Yunita

“…Backgroud: The creative culinary industry plays a vital role in enhancing tourist destination attraction, serving as a cultural and culinary gateway for travelers. With growing awareness of sustainability and environmental concerns, it becomes imperative to investigate the development of sustainability strategies in this sector. …”
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Autism spectrum disorder: definition, global epidemiology, prevalence in Poland and worldwide, and heredity by Martyna Zakrocka, Marta Gruszka, Paulina Polańska, Maria Kubicka

“…Ongoing research and culturally tailored strategies are essential to improve diagnosis, support, and quality of life for individuals struggling with this condition. …”
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Turkish suicide patterns: Longitudinal analysis of suicide trends in Turkiye (2000 -2022) by Ayhan Tabur, Feruza Turan Sonmez

“…Addressing broader socioeconomic and cultural factors is essential for effective suicide prevention strategies. …”
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Contextual determinates of under-five malnutrition in mining communities: a critical review of nutrition interventions for optimal impact by Herbert Tato Nyirenda, David Mulenga, Hilda Nyambe-Silavwe

“…Understanding these factors is essential for designing interventions that are not only effective but also sustainable and culturally appropriate.…”
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