Recent advances in the extraction technology and bioactivity of lentinan by Sizhu Ren, Chunyan Xie, Hua Liu, Liyan Li, Xiaoling Han, Wenjie Chen

“…Many advanced technologies have been applied for lentinan extraction, such as ultrasonic-assisted, microwave-assisted, and enzyme-assisted extractions. However, their advantages and disadvantages differ, which directly affect the structural and functional properties of lentinan. …”
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Exploring Echinacea angustifolia for anti-viral compounds against Zika virus RNA-dependent RNA polymerase: a computational study by Mai M. El-Daly, Leena H. Bajrai, Thamir A. Alandijany, Isra M. Alsaady, Hattan S. Gattan, Meshari M. Alhamdan, Vivek Dhar Dwivedi, Esam I. Azhar

“…This study explores the potential of compounds from Echinacea angustifolia (E. angustifolia) to inhibit the activity of ZIKV’s RNA-dependent RNA polymerase (RDRP), a key enzyme in the viral replication process and an ideal candidate for antiviral therapy. …”
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Efficient biosynthesis of β-caryophyllene by engineered Yarrowia lipolytica by Young-Kyoung Park, Lucie Studena, Piotr Hapeta, Ramdane Haddouche, David J. Bell, Pablo Torres-Montero, Jose Luis Martinez, Jean-Marc Nicaud, Adriana Botes, Rodrigo Ledesma-Amaro

“…Through the modulation of key enzyme expression, we successfully demonstrated an improvement in β-caryophyllene production. …”
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Dietary inclusion of African bush mango (Irvingia gabonensis) seed improves sexual performance in sexually inexperienced rats by Olasunkanmi Omojokun, Akindele Famurewa, Ganiyu Oboh, Jemima Enang, Stella Ijeh

“…The experiment lasted for 21 days after which behavioral studies involving sexual and anxiety indices/measurements, lipid peroxidation, and enzyme assays (arginase and phosphodiesterase-5 [PDE-5]) were conducted. …”
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Exploring Quality of Life in Adults Living With Late-onset Pompe Disease: A Combined Quantitative and Qualitative Analysis of Patient Perceptions from Australia, France, Italy, and... by Holly Lumgair, Lisa Bashorum, Alasdair MacCulloch, Elizabeth Minas, George Timmins, Drago Bratkovic, Richard Perry, Medi Stone, Vasileios Blazos, Elisabetta Conti, Raymond Saich

“…Physical functioning scores were generally stable in respondents who had been receiving enzyme replacement therapy (ERT) for 1-15 years, but those who had received ERT for >15 years had lower scores. …”
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NT5E (CD73) as a prognostic biomarker and therapeutic target associated with immune infiltration in lung adenocarcinoma by Leyan Chen, Tuoya Qi, Bishu Zhang, Xuelong Wang, Mingfeng Zheng

“…NT5E, an ecto-5’-nucleotidase enzyme, has been implicated in cancer progression, particularly in efferocytosis. …”
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Pain in pancreatitis: issue and ways out by A. V. Okhlobystin

“…Drug therapy of pancreatic pain is based on digestive enzymes, antisecretory and analgetic agents.…”
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Secretory phospholipase A2 inhibitory activity in Tragia hispida: A novel and promising therapeutic agent for dengue fever by D.V. Dayangi Hemalika, U.G. Chandrika, Ajita M. Abeysekera, Sameera R. Samarakoon, Ananda Wijewickrama, Gathsaurie Neelika Malavige

“…Secretory phospholipase A2 (sPLA2), an inflammatory enzyme, plays a critical role in producing platelet-activating factor (PAF), a key mediator of vascular leak. …”
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Salivary Levels of Interleukin-17 in Iranian Patients With Systemic Sclerosis by Ali Tadayon, Javad Moayedi, Mohamad Ali Nazarinia, Mahyar Malekzadeh, Sara Kazemi, Maryam Mardani

“…The salivary levels of IL-17 in all samples were measured using a commercially available enzyme–linked immunosorbent assay (ELISA) kit. The mean salivary levels of IL-17 in patients with SSc were significantly higher than the control group (199.6±38.9 pg/mL vs. 112.7±39.4 pg/mL, P<0.0001). …”
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Bee Venom—A Potential Complementary Medicine Candidate for SARS-CoV-2 Infections by Keneth Iceland, Kasozi, Simon Peter, Musinguzi

“…SARS-CoV-2 directly interacts with angiotensin-converting enzyme 2 (ACE-2) receptors in the human body. Complementary therapies that impact on expression of IgE and IgG antibodies, including administration of bee venom (BV), have efficacy in the management of arthritis, and Parkinson’s disease. …”
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Extraction of Saponins from Xanthoceras sorbifolium Bunge Leaves Using Deep Eutectic Solvents and Their Inhibitory Activity against Postharvest Pathogenic Fungi by Xue LI, Xinyu CHU, Erzheng SU, Jiahong WANG

“…Among the pathogenic fungi studied, the spore germination of Rhizopus oryzae was inhibited with the best effect, the inhibition rate reached 78.76%±2.56%, and XLs saponin inhibited the growth of the fungus by suppressing the antioxidant enzyme activity. These results suggest promising applications for XLs saponins in the postharvest preservation of fruits and vegetables.…”
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Typical and atypical metabolic characteristics of three iridaceae isoflavone components: in vitro and in silico studies by Jifeng Gu, Jifeng Gu, Huishan Zhang, Mei Wang, Yuyang Zhou, Yuyang Zhou, Zhipeng Deng, Rong Shi

“…However, the metabolic characteristics of isoflavone aglycones remain unclear.MethodsIn this study, human liver microsomes (HLMs) and Cytochrome P450 (CYP) recombinant enzymes were used to study the metabolic stability, identify the metabolic pathways and enzyme kinetics of these three components, and elucidate their possible binding sites through molecular docking.ResultsWhen tectorigenin, irigenin, and irisflorentin were co-incubated with HLMs and CYP recombinant enzymes, hydroxylation metabolite for tectorigenin, demethylated metabolite for irigenin, and 6,7-dihydroxy-5,3′,4′,5′-tetramethoxy isoflavone originating from irisflorentin were identified. …”
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Proximity extension assay revealed novel inflammatory biomarkers for follicular development and ovarian function: a prospective controlled study combining serum and follicular flui... by Chong Wang, Ying Feng, Yu Chen, Xianhua Lin, Xiangjuan Li

“…Olink proteomic proximity extension assay (PEA) technology was used to compare the differentially expressed proteins (DEPs), and patients were divided into two subgroups—the normal ovarian reserve (NOR) group and the DOR group—for further bioinformatics analysis and verification by enzyme-linked immunosorbent assay (ELISA).ResultsIn total, 16 DEPs were detected between the mature group and the immature group (FF), and 11 DEPs were detected between the ovulation group and the menstruation group (serum). …”
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The RNA M5C methyltransferase NSUN2 promotes progression of hepatocellular carcinoma by enhancing PKM2-mediated glycolysis by Qin Qi, Rui Zhong, Yan Huang, Yong Tang, Xiao-wen Zhang, Chang Liu, Chun-fang Gao, Lin Zhou, Jian Yu, Lu-yi Wu

“…Mechanistically, our data revealed that PKM2, a terminal enzyme in the glycolytic pathway, is a downstream target of NSUN2-mediated m5C modification. …”
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Association of leptin receptor Gln223Arg genetic polymorphism with obesity and non-alcoholic fatty liver disease by A. V. Morozova, N. V. Mal'tseva, Y. A. Gorbatovsky, O. F. Lykova, S. V. Arkhipova

“…Cholesterol and triglycerides serum levels were estimated by enzyme colorimetric method, leptin concentration — by immunoenzyme method. …”
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Anti-motilities and anti-biofilm effects of Ageratum conyzoides L. methanol extract by Eli Compaoré, Moussa Compaoré, Vincent Ouédraogo, Ablassé Rouamba, Alimata Bancé, Mignini R. Dofini, Martin Kiendrebeogo

“…The anti-biofilm effect was conducted in microtiter plates using the crystal violet method. The antioxidant and enzyme inhibition activities were evaluated using 2,2-diphényl-1-picrylhydrazyl; 2,2’-azino-bis (3-éthylbenzothiazoline-6-sulfonic acid), Ferric Reducing Antioxidant Power and conducting lipoxygenase test. …”
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Health-Related Quality-of-Life Utility Values in Adults With Late-Onset Pompe Disease: Analyses of EQ-5D Data From the PROPEL Clinical Trial by Alison Griffiths, Simon Shohet, Neil Johnson, Alasdair MacCulloch

“…Clinical evidence suggests that enzyme replacement therapy improves health outcomes for adults with late-onset Pompe disease (LOPD). …”
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Abnormal methylation of Mill1 gene regulates osteogenic differentiation involved in various phenotypes of skeletal fluorosis in rats and methionine intervention by Niannian Chen, Jing Zhang, Congyu Yin, Yudan Liao, Lei Song, Ting Hu, Xueli Pan

“…Subsequently, we validated the regulation of Mill1 gene methylation using DNMT1 and TET2 enzyme inhibitors. Furthermore, we knockdown and overexpression experiments confirmed its downregulation inhibited osteogenic differentiation, whereas osteogenic differentiation was promoted by its overexpression. …”
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Assessment of Blood Pressure Control Status Among Hypertensive Patients Attending Rwandan District Hospital NCD Clinics: A Retrospective Follow-Up Study by Evariste Ntaganda, Ziad El-Khatib, Regine Mugeni, Boniface Nsengiyumva, Clarisse Musanabaganwa, James Gafirita, Francois Uwinkindi, Richard Kalisa

“…Factors significantly associated with uncontrolled BP included BMI ≥ 30 kg/m2 (p < 0.001), use of Angiotensin-converting enzyme (ACE) inhibitors (p = 0.01), use of four antihypertensive drugs (p = 0.013), and missing an NCD clinic appointment (p < 0.001). …”
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CADD based designing and biological evaluation of novel triazole based thiazolidinedione coumarin hybrids as antidiabetic agent by Anchal Sharma, Anmol Narang, Nitish Kumar, Rupali Rana, Megha, Pooja, Muskan Dhir, Harmandeep Kaur Gulati, Jyoti, Aanchal Khanna, Jatinder Vir Singh, Sukhraj Kaur, Preet Mohinder Singh Bedi

“…In vitro evaluation against the α-glucosidase enzyme yielded an IC 50 value of 1.49 µg/ml. In vivo studies demonstrated that Compound I-1 significantly reduced blood glucose levels, with values of 94.15 mg/dL and 74.60 mg/dL at doses of 10 mg/kg and 20 mg/kg, respectively. …”
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